International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Identification of a pr 1-like Gene of Entomopathogenic Fungus, Beauveria bassiana F-101 Isolated from Thecodiplosis japonensis

  • Shin Sang Chul (Division of Forest Insect Pests and Diseases, Korea Forest Research Institute) ;
  • Roh Jong Yul (School of Agricultural Biotechnology, Seoul National University) ;
  • Shim Hee Jin (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim Soon Kee (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim Chul Su (Division of Forest Insect Pests and Diseases, Korea Forest Research Institute) ;
  • Park Il Kwon (Division of Forest Insect Pests and Diseases, Korea Forest Research Institute) ;
  • Jeon Mun Jang (Department of Life & Environment, College of Natural Resources, Daegu University) ;
  • Je Yeon Ho (School of Agricultural Biotechnology, Seoul National University)
  • Published : 2005.06.01
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Abstract

Beauveria bassiana F-101, which has high toxicity toward Acantholyda parki as well as Thecodiplosis japonensis, was an isolate to develop an alternative control system against the major forest pests. Up to now, in B. bassiana, only one pr1 gene has been isolated and characterized. Therefore, we here reported the identification of a pr1-like gene, which would be a factor of toxicity from B. bassiana F-101. The oligonucleotides for the amplification of the pr1-like gene, were chosen based on the conserved regions of the subtilisin family enzymes, pr1 genes of B. bassiana and Metarhizium anisopliae, and proteinase K of Tritirachium album. The cloned PCR fragment had 1111 bp including 52 bp intron. The deduced Pr1-like peptide showed a low identity with Pr1s of entomopathogenic fungi such as B. bassiana Pr1 (BbPr1) and M. anisopliae Pr1 (MaPr1) as well as the proteinase K of T. album (TaPrK). Instead, the deduced peptide had a substantially high amino acid sequence identity $(>65\%)$ with the serine proteases of Magnaporthe grisea (MgSPM1) and Podospora anserina (PaPspA). These results, therefore, appear to suggest that the putative Pr1-like peptide of B. bassiana F-101 belongs to the subtilisin-like serine protease family and may be a novel gene.

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References

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