Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Degradation of Phenanthrene by Bacterial Strains Isolated from Soil in Oil Refinery Fields in Korea

  • KIM JEONG DONG (Institute of Industrial and Biotechnology, Department of Biological Engineering, Inha University) ;
  • SHIM SU HYEUN (Institute of Industrial and Biotechnology, Department of Biological Engineering, Inha University) ;
  • LEE CHOUL GYUN (Institute of Industrial and Biotechnology, Department of Biological Engineering, Inha University)
  • Published : 2005.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

The degradation of phenanthrene, a model PAH compound, by microorganisms either in the mixed culture or individual strain, isolated from oil-contaminated soil in oil refmery vicinity sites, was examined. The effects of pH, temperature, initial concentration of phenanthrene, and the addition of carbon sources on biodegradation potential were also investigated. Results showed that soil samples collected from four oil refinery sites in Korea had different degrees of PAH contamination and different indigenous phenanthrene-degrading microorganisms. The optimal conditions for phenanthrene biodegradation were determined to be 30$^{circ}C$ and pH 7.0. A significantly positive relationship was observed between the microbial growth and the rate of phenanthrene degradation. However, the phenanthrene biodegradation capability of the mixed culture was not related to the degree of PAH contamination in soil. In low phenanthrene concentration, the growth and biodegradation rates of the mixed cultures did not increase over those of the individual strain, especially IC10. High concentration of phenanthrene inhibited the growth of microbial strains and biodegradation of phenanthrene, but was less inhibitory on the mixed culture. Finally, when non-ionic surfactants such as Brij 30 and Brij 35 were present at the level above critical micelle concentrations (CMCs), phenanthrene degradation was completely inhibited and delayed by the addition of Triton X100 and Triton N101.

Keywords

References

  1. Atlas, R. 1981. Microbiol degradation of petroleum hydrocarbons: An environmental perspective. Microb. Rev. 45: 180- 209
  2. Baek, K.-H., H.-S. Kim, S.-H. Moon, I-S. Lee, H.-M Oh, and B.-D. Yoon. 2004. Effects of soil types on the biodegradation of crude oil by Nocardia sp. H17-1. J. Microbiol. Biotechnol. 14: 901- 905
  3. Bauer, J. E. and D, G. Capone. 1988. Effects of co-occurring aromatic hydrocarbons on degradation of individual polycyclic aromatic hydrocarbons in marine sediments slurries. Appl. Environ. Microbiol. 54: 1649- 1655
  4. Boldrin, B., A. Tiehm, and C. Friasche. 1993. Degradation of phenanthrene, fluorine, fluoranthene, and pyrene by a Mycobacterium sp. Appl. Environ. Microbiol. 59: 1927-1930
  5. Catallo, W. J. and R. J. Portier. 1992. Use of indigenous and adapted Microbial assemblages in the removal of organic chemicals from soils and sediments. Wat. Sci. Technol. 25: 229-237
  6. Cerniglia, C. 1992. Biodegradation of polycyclic aromatic hydrocarbons. Biodegradation 3: 351 - 368 https://doi.org/10.1007/BF00129093
  7. Chaineau, C. H., J. Moreal, J. Dupont, E. Bury, and J. Oudot. 1999. Comparison of the fuel oil biodegradation potential of hydrocarbon-assimilating microorganisms isolated from a temperate agricultural soil. Sci. Tot. Environ. 227: 237-247 https://doi.org/10.1016/S0048-9697(99)00033-9
  8. Cowell, R. R. and J. D. Walker. 1977. Ecological aspects of microbial degradation of petroleum in the marine environment. Crit. Rev. Microbiol. 4: 423-445
  9. Dagher, F., E. Deziel, P. Lirette, G. Paquette, J. Bisaillon, and R. ViIlemur. 1996. Comparative study of five polycyclic aromatic hydrocarbon degrading bacterial strains isolated from contaminated soil. Can. J. Microbiol. 43: 368- 377 https://doi.org/10.1139/m97-051
  10. Dyksterhouse, S., J. Gray, R. Herwig, J. Lara, and J. Staley. 1995. Cycloclasticus pigetti gen. nov., and aromatic hydrocarbon-degradation bacterium from marine sediments. Appl. Environ. Microbiol. 45: 116-123
  11. Ellis, B. P., P. Harold, and H. Kornberg. 1991. Biodegradation of creosote contaminated site. Environ. Technol. 12: 447-459 https://doi.org/10.1080/09593339109385029
  12. Guha, S. and P. R. Jaffe. 1996. Biodegradation kinetics of phenanthrene partitioned into the micellar phase of nonionic surfactants. Environ. Sci. Technol. 30: 605- 611 https://doi.org/10.1021/es950385z
  13. Han, K., Y.-T. Jung, and S.-Y. Son. 2003. Phylogenetic analysis of phenanthrene-degrading Sphingomonas. J. Microbiol. Biotechnol. 13: 942- 948
  14. Hiraishi, A. and Y. Ueda. 1994. Intrageneric structure of the genus Rhodobacter: Transfer of Rhodobacter sulfidophllus and related marine species to the genus Rhodovulum gen. nov. Int. J. Syst. Bacteriol. 44: 15- 23 https://doi.org/10.1099/00207713-44-1-15
  15. Kastner, M., M. Breuer-Jamrnali, and B. Mahro. 1994. Enumeration and characterization of the soil microflora from hydrocarbon-contaminated soil site able to mineralized polycyclic aromatic hydrocarbons (PAH). Appl. Environ. Biotechnol. 41: 267 - 273 https://doi.org/10.1007/BF00186971
  16. Kobayashi, H. and B. E. Ritmann. 1982. Microbiol removal of hazardous organic compounds. Environ. Sci. Technol. 16: 170- 183 https://doi.org/10.1021/es00097a724
  17. Lethomaki, M. and S. Niemela. 1975. Improving Microbiol degradation of oil in soil. Ambio 4: 126- 129
  18. Lin, C. B. Flesher, W. C. Capman, and R. I. Amann. 1994. Taxon specific hybridization probes for tiber-digesting bacteria suggest novel gut-associated Fibrobacter. Syst. Appl. Microbiol. 17: 218- 227
  19. Lloyd-Jones, G. and D. W. Hunter. 1997. Characterization of fluoranthene- and pyrene-degrading Mycobacterium-like strains by RAPD and SSU sequencing. FEMS Microbiol. Lett. 153: 51-56 https://doi.org/10.1111/j.1574-6968.1997.tb10462.x
  20. Mackey, D., W. Y. Shiu, and K. C. Ma. 1992. Polynuclear aromatic hydrocarbons, polychlorinated dioxins, dibenzofurans. In: Illustrated Handbook of Physical Chemical Properties and Environment Fate for Organic Chemicals. vol II. CRC Press LLC, Boca Raton, FL, U.S.A
  21. Mueller, J., C. Cerniglia, and P. Pritchard. 1997. Bioremediation of environments contaminated by polycyclic aromatic hydrocarbons, pp. 125- 194. In R. Crawford and D. Crawford (eds.), Bioremediation: Principles and Practices. Cambridge University Press, New York, U.S.A
  22. Mueller. J., P. Chapman, and P. Pritchard. 1989. Creosotecontaminated sites: Their potential for biodegradation. Environ. Sci. Technol. 23: 1197- 1201 https://doi.org/10.1021/es00068a003
  23. Nigam. P., G. Armour, I. M. Banat, D. Singh, and R. Marchant. 2000. Physical removal of textile dyes and solidstate fermentation of dye-adsorbed agricultural residues. Bioresour. Technol. 72: 219- 226 https://doi.org/10.1016/S0960-8524(99)00123-6
  24. Oh, Y.-S., D.-D. Kim, and S.-J. Kim. 2003. Effectiveness of bioremediation on oil-contaminated sand in intertidal zone. J. Microbiol. Biotechnol. 13: 437- 443
  25. Phalhmann, R. and O. Pelkonen. 1987. Mutagenicity studies of different polycyclic aromatic hydrocarbons: The significance of enzymatic factors and molecular structures. Carcinogenesis 8: 773-778 https://doi.org/10.1093/carcin/8.6.773
  26. Ramsay, M. A., R. P. J. Swannells, W. A. Shipton. N. C. Duke, and R. T. Hill. 2000. Effect of bioremediation on the Microbiol community in oiled mangrove sediments. Mar. Pollut. Bull. 41: 413-419 https://doi.org/10.1016/S0025-326X(00)00137-5
  27. Saitou, N. and M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406- 425
  28. Schocken, M. and D. Gibson. 1984. Bacteria oxidation of polycyclic aromatic hydrocarbons accnaphthene and acenaphthylene. Appl. Environ. Microbiol. 48: 10- 16
  29. Stackebrandt, E. and W. Liesack 1993. Nucleic acids and classification. In M. Goodfellow and A. G. O. Donnell (eds.). Handbook of New Bacterial Systematics. Academic Press, London, U.K
  30. Tam, N. F. Y., L. Ke, X. H. Wang, and Y. S. Wang. 2001. Contamination of polycyclic aromatic hydrocarbons in surface sediments of mangrove swamps. Environ. Pollut. 114: 255- 263 https://doi.org/10.1016/S0269-7491(00)00212-8
  31. Trzesicka-Mlynartz, D. and O. P. Ward. 1995. Degradation of polycyclic aromatic hydrocarbons (PAHs) by a mixed culture and its component pure cultures, obtained from PAHcontaminated soil. Can. J. Microbiol. 41: 470- 476 https://doi.org/10.1139/m95-063
  32. Verschueren, K. 1983. Handbook of Environmental Data on Organic Chemicals. Van Nostrand of Reinhold, New York, U.S.A
  33. Volkering, F., A. M. Breure, J. G. V. Andel, and W. H. Rulkens. 1995. Influence of nonionic surfactants on bioavai lability and biodegradation of polycyclic aromatic hydrocarbons. Appl. Environ. Microbiol. 61: 1699- 1705
  34. Wayne, L. G., R. C. Good, A. Tsang, and R. Butler. 1993. Serovar determination and molecular taxonomic correlation in Mycohacterlum avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum: A cooperative study of the international working group on mycobacterial taxonomy. Int. J. Syst. Bacteriol. 43: 482- 493 https://doi.org/10.1099/00207713-43-3-482
  35. White, K. L. 1986. An overview of immunotoxicology and carcinogenic polycyclic aromatic hydrocarbons. Environ. Carcinogenesis Rev. C4: 163- 202
  36. Wild, S. R. and K. C. Jones. 1986. Biological and abiotic losses of polynuclear hydrocarbons from soils freshly amended with sewage sludge. Environ. Toxicol. Chem. 12: 5- 12 https://doi.org/10.1897/1552-8618(1993)12[5:BAALOP]2.0.CO;2
  37. Wilson, S. C. and K. C. Jones. 1993. Remediation of soil contaminated with polynuclear aromatic hydrocarbons (PAHs): A review. Environ. Pollut. 35: 229- 249
  38. Woo, S. H. and J. M. Park. 2004. Biodegradation of aromatic compounds from soil by drum bioreactor system. J. Microbiol. Biotechnol. 14: 435 - 441
  39. Yuan, S. Y., S. H. Wei. and B. V. Chang. 2000. Biodegradation of polycyclic aromatic hydrocarbons by a mixed culture. Chemosphere 41: 1463- 1468 https://doi.org/10.1016/S0045-6535(99)00522-6
  40. Yuan, S. Y., S. H. Wei, and B. V Chang. 2001. Biodegradation of phenanthrene in river sediment. Chemosphere 43: 273-278 https://doi.org/10.1016/S0045-6535(00)00139-9
  41. Yoon, J.-H., S.-T. Lee, S.-B. Kim, W.-Y. Kim, M. Goodfellow, and Y.-H. Park. 1997. Restriction fragment length polymorphisms analysis of PCR-amplified 16S ribosomal DNA for rapid identification of Saccharomonospora strains. Int. J. Syet. Bacteriol. 47: 111- 114 https://doi.org/10.1099/00207713-47-1-111
  42. Zaidi, B. R. and S. H. Imam. 1999. Factors affecting Microbiol degradation of polycyclic aromatic hydrocarbon phenanthrene in the Caribbean coastal water. Mar. Pollut. Bull. 38: 732-742