Purification and Characterization of Chitinase from Paenibacillus illinoisensis KJA-424

  • JUNG WOO JIN (Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology Institute of Agricultural Science and Technology, Chonnam National University) ;
  • KUK JU HEE (Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology Institute of Agricultural Science and Technology, Chonnam National University) ;
  • KIM KIL YONG (Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology Institute of Agricultural Science and Technology, Chonnam National University) ;
  • KIM TAE HWAN (Department of Animal Science, College of Agriculture and Life Science, Chonnam National University) ;
  • PARK RO DONG (Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology Institute of Agricultural Science and Technology, Chonnam National University)
  • Published : 2005.04.01

Abstract

A chitinase was purified from the culture supernatant of Paenibacillus illinoisensis KJA-424 by protein precipitation, DEAE-Sephadex anion-exchange chromatography, and Sephadex G-150 gel filtration. The molecular weight of the purified chitinase was 54 kDa on SDS-PAGE and activity staining. Optimal pH and temperature were pH 5.0 and 60$^{circ}$C, the presence of 10 ruM Ag$^{+}$ and Hg$^{2+}$ inhibited the activity by $92.1/%$ and $97.7/%$, and the K$_{m}$ and V$_{max}$ values were 1.12 mg chitin mrl and 1.48$\mu$mol GlcNAc min$^{-1}$, respectively. The enzyme hydrolyzed tetramer to dimer, pentamer to dimer and trimer, and hexamer to dimer, trimer and tetramer, indicating an endo-splitting mechanism. The chitinase had no hydrolytic activity toward dimer and trimer. The chitinase inhibited the mycelial growth of Rhizoctonia solani, suggesting an antifungal property.

Keywords

References

  1. Arlorio, M., A. Ludwig, T. Boller, and P. Bonfante. 1992. Inhibition of fungal growth by plant chitinase and 1,3- glucanase. Protoplasma 171: 34- 43 https://doi.org/10.1007/BF01379278
  2. Bagnasco, P., L. De La Fuente, G. Gualtieri, F. Noya, and A. Arias. 1998. Fluoresent Pseudomonas spp. as biocontrol agents against forage legume root pathogenic fungi. Soil Biol. Biochem. 30: 1317- 1322 https://doi.org/10.1016/S0038-0717(98)00003-0
  3. Bradford, M. M. 1976. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal. Biochem. 72: 248- 254 https://doi.org/10.1016/0003-2697(76)90527-3
  4. Chet, I. 1987. Trichoderma - Application, mode of action, and potentials biological agent of soil-borne plant pathogenic fungi, pp. 137- 160. In I. Chet (ed.), Innovative Approaches to Plant Disease Control. John Wiley & Sons. New York, U.S.A
  5. Deane, E. E., J. M. Whipps, J. M. Lynch, and J. F. Peberdy. 1998. The purification and characterization of a Trichoderma harzianum exochitinase. Biochem. Biophys. Acta 1383: 101-110 https://doi.org/10.1016/S0167-4838(97)00183-0
  6. Godoy. G, R. Rodriguez-Kabana, and G. Morgan-Jones. 1982. Parasitism of eggs of Heterodera glycines and Meloidogyne arenaria by fungi isolated from cysts of H. glycines. Nematropica 12: 111- 119
  7. Han, O. K., E. T. Lee, and S. D. Kim. 2001. Chitinase of multifunctional antagonistic bacterium Bacillus amyloliquefaciens 7079 against phytopathogenic fungi. Kor. J. Appl. Microbiol. Biotechnol. 29: 142- 148
  8. Han, O. K., E. T. Lee, Y. S. Lee, and S. D. Kim. 2003. Purification of chitinase from an antagonist bacterium Bacillus sp. 7079 and pro-inflammatory cytokine gene expression by PCTC. J. Microbiol. Biotechnol. 13: 77- 84
  9. Harman, G. E., C. K. Hayes, M. Lorito, R. M. Broadway, A. Di Pietro, C. Peterbauer, and A. Tronsmo. 1993. Chitinolytic enzymes of Trichoderma harzianum: Purification of chitobiosidase and endochitinase. Phytopathology 83: 313- 318 https://doi.org/10.1094/Phyto-83-313
  10. Hong, B. S., H. G. Yoon, D. H. Shin, and H. Y. Cho. 1996. Purification and characterization of thermostable chitinase from Bacillus licheniforms KFB-C14. Kor. J. Appl. Microbiol. Biotechnol. 24: 567 - 573
  11. Huang, J. H., C. J. Chen, and Y. C. Su. 1996. Production of chitinolytic enzymes from a novel species of Aeromonas. J. Ind. Microbiol. 17: 89- 95 https://doi.org/10.1007/BF01570049
  12. Jo, Y. Y., K. J. Jo, Y. L. Jin, W. J. Jung, J. H. Kuk, K. Y. Kim, T. H. Kim, and R. D. Park. 2003. Characterization of endochitosanase-producing Bacillus cereus P16. J. Microbiol. Biotechnol. 13: 960- 968
  13. Jung, W. J., S. J. Jung, K. N. An, Y. L. Jin, R. D. Park, K. Y. Kim, B. K. Shon, and T. H. Kim. 2002. Effect of chitinase-producing Paenibacillus illinoisensis KJA-424 on egg hatching of root-knot nematode (Meloidogyne incognita). J. Microbiol. Biotechnol. 12: 865- 871
  14. Jung, W. J., K. N. An, Y. L. Jin, R. D. Park, K. T. Lim, K. Y. Kim, and T. H. Kim. 2003. Biological control of dampingoff caused by Rhizoctonia solani using chitinase-producing Paenibacillus illinoisensis KJA-424. Soil Biol. Biochem. 35: 1261- 1264 https://doi.org/10.1016/S0038-0717(03)00187-1
  15. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680- 683 https://doi.org/10.1038/227680a0
  16. Lee, H. S., H. J. Lee, S. W. Choi, S. Her, and D. H. Oh. 1997. Purification and characterization of antifungal chitinase from Pseudomonas sp. YHS-A2. J. Microbiol, Biotechnol. 7: 107-113
  17. Lee, J. S., D. S. Joo, S. Y. Cho, J. H. Ha, and E. H. Lee. 2000. Purification and characterization of extracelluar chitinase produced by marine bacterium, Bacillus sp. LJ-25. J. Microbiol. Biotechnol. 10: 307 - 311
  18. Lee, K. P., C. N. Kim, J. H. Yu, and D. H. Oh. 1990. The production and purification of chitinase from Aeromonas salmonicida YA7-625. Kor. J. Appl. Microbiol. Biotechnol. 18: 599- 606
  19. Lee, E. T. and S. D. Kim. 1999. Purification and charaterization of antifungal chitinase from indigenous antagonistic microorganism Serratia sp, 3095. Agric. Chem. Biotechnol. 42: 7 -11
  20. Lingappa, Y. and J. L. Lockwood. 1962. Chitin media for selective isolation and culture of actinomycetes. Phytopathology 52: 317-323
  21. Moder, W., A. Bunk, A. Albrecht, H. Doostdar, R. P. Niedz, R. E. McDonald, R. T. Mayer, and W. F. Osswald. 1999. Characterization of acidic chitinase from culture medium of sweet orange callus tissue. J. Plant Physiol. 154: 296- 301 https://doi.org/10.1016/S0176-1617(99)80171-0
  22. Park, S. K., H. Y. Lee, and J. W. Huh. 1995. Producton and some properties of chitinolytic enzymes by antagonistic bacteria. Kor. J. Plant Pathol. 11: 258- 264
  23. Punja, Z. K. and Y. Y. Zhang. 1993. Plant chitinase and their role in resistance to fungal disease. J. Nematol 25: 526-540
  24. Sakai, K., A. Yokota, H. Kurokawa, M, Wakayama, and M. Moriguchi. 1998. Purification and characterization of three thermostable endochitinases of a noble Bacillus strain, MH-1, isolated from chitin-containing compost. Appl. Environ. Microbiol. 64: 3397- 3402
  25. Singh, P. P., Y. C. Shin, C. S. Park, and Y. R. Chung. 1999. Biological control of fusarium wilt of cucumber by chitinoiytic bacteria. Phytopathology 89: 92- 99 https://doi.org/10.1094/PHYTO.1999.89.1.92
  26. Spiegel, Y., I. Chet, and E. Cohn. 1987. Use of chitin for controlling plant-parasitic nematodes II. Mode of action. Plant Soil 98: 337-345 https://doi.org/10.1007/BF02378355
  27. Tantimavanich, S., S. Pantuwatana, A. Bhumiratana, and W. Pannbangred. 1998. Multiple chitinase enzymes from a single gene of Bacillus licheniformis TP-1. J. Ferment. Bioeng. 85: 259- 265 https://doi.org/10.1016/S0922-338X(97)85672-3
  28. Trudel, J. and A. Asselin. 1989. Detection of chitinase activity after polyacrylamide gel electrophoresis. Anal. Biochem. 178: 362- 366 https://doi.org/10.1016/0003-2697(89)90653-2
  29. Ulhoa, C. J. and J. F. Peberdy. 1992. Purification and some property of the excellular chitinase produced by Trichoderma harzianium. Enzyme Microb. Technol. 14: 236- 240 https://doi.org/10.1016/0141-0229(92)90072-V
  30. Wang, S. L., T. C. Yieh, and I. L. Shih. 1999. Production of antifungal compounds by Pseudomonas aeruginosa K-187 using shrimp and crab shell powder as a carbon source. Enzyme Microb. Technol. 25: 142- 148 https://doi.org/10.1016/S0141-0229(99)00024-1
  31. Wiwat, C., S. Patcharaporn, and B. Amarat. 1999. Purification and characterization of chitinase from Bacillus circulans No. 4.1. Curr. Microbiol. 39: 134- 140 https://doi.org/10.1007/s002849900434
  32. Yabuki, M., K. Mizushina, T. Amatatsu, A. Ando, T. Fujii, M. Shimada, and M. Yamashida. 1986. Purification and characterization of chitinase and chitobiose produced by Aeromonas hydrophila subsp. Anaerogenes A52. J. Gen. Appl. Microbiol. 32: 25- 38 https://doi.org/10.2323/jgam.32.25