Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Identification of Aspergillus Strain with Antifungal Activity Against Phytophthora Species

  • KANG SUNG WOO (Department of Chemical and Biological Engineering, Korea University) ;
  • HONG SUK IN (Graduate School of Biotechnology, Korea University) ;
  • KIM SEUNG WOOK (Applied Rheology Center (ARC), Korea University)
  • Published : 2005.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

Fungal strain CGF was isolated from the soil of ChungNam Province, South Korea. Based on the 28S rDNA sequence analysis and the sequence of the internal transcribed spacer (ITS) region of ribosomal DNA, together with morphological and cultural characteristics, this strain was identified as Aspergillus sclerotiorum and renamed Aspergillus sclerotiorum CGF. This is the first strain of Aspergillus sclerotiorum identified in Korea. When the antifungal activity of A. sclerotiorum CGF was evaluated, among the phytopathogenic fungi, mycelial growth of only Phytophthora species was inhibited. Oermination of P. capsid zoospore was also inhibited. The bioactive compound of A. sclerotiorum CGF was highly thermo- and pH-stable.

Keywords

References

  1. Agrios, G. N. 1997. Plant Pathology, pp. 635. 4th Ed. Academic Press, 525 B St. Suite 1900, San Diego, U.S.A
  2. Christensen, M. 1982. The Aspergillus ochraceus group: Two new species from western soils and synoptic key. Mycologia 74: 210- 225 https://doi.org/10.2307/3792887
  3. De Waard, M. A., S. G. Georgopoulos, D. W. Hollomon, H. Ishii, P. Leroux, N. N. Ragsdale, and F. J. Schwinn. 1993. Chemical control of plant diseases: Problems and prospects. Annu. Rev. Phytopathol. 31: 403- 421 https://doi.org/10.1146/annurev.py.31.090193.002155
  4. Erwin, D. C. and O. K. Ribeiro. 1996. Phytophthora Diseases Worldwide, pp. 562. APS Press, St. Paul, Minnesota, U.S.A
  5. Gullino, M. L., P. Leroux, and C. M. Smith. 2000. Uses and challenges of novel compounds for plant disease control. Crop Protect. 19: 1- 11 https://doi.org/10.1016/S0261-2194(99)00095-2
  6. Jee, H. J., W. G. Kim, J. Y. Kim, and S. E. Lim. 1998. Unrecorded Phytophthora diseases of flowering plants caused by Phytophthora nicotianae in Korea. Kor. J. Plant Parhol. 14: 452-457
  7. Jee, H. J., W. D. Cho, and C. H. Kim. 2000. Phytophthora Diseuses in Korea. National Institute of Agricultural Science and Technology (NIAST), RDA, Suwon, Korea
  8. Kim, B. J., G. J. Choi, K. Y. Cho, H. J. Yang, C. S. Shin, C. H. Lee, and Y. G. Lim. 2002. Antifungal activity against Plasmodiophora brassicae causing club root. J. Microbiol. Biotechnol. 12: 1022- 1025
  9. Kim, C. H. 2002. Review of disease incidence of major crops in 2001. Res. Plant Dis. 8: 1- 10 https://doi.org/10.5423/RPD.2002.8.1.001
  10. Knight, S. C., V. M. Anthony, A. M. Brady, A. J. Greenland, S. P. Heaney, D. C. Murray, K. A. Powell, M. A. Schulz, C. A. Spinks, P. A. Worthington, and D. Youle. 1997. Rationale and perspectives on the development of fungicides. Annu. Rev. Phytopathol. 35: 349- 372 https://doi.org/10.1146/annurev.phyto.35.1.349
  11. Kwon, S. B., H. J. Jee, S. B. Bang, K. K. Lee, and C. K. Hong. 1999. Phytophthora root rot of Ligularia fishcheri caused by P. drechsleri. Plant Dis. Agricul. 5: 58- 60
  12. Lange, L., J. Breinholt, F. W. Rasmussen, and R. I. Nielsen. 1993. Microbial fungicides - the natural choice. Pest. Sci. 39: 155- 160 https://doi.org/10.1002/ps.2780390209
  13. Lee, C. H., B. J. Kim, G. J. Choi, K. Y. Cho, H. J. Yang, C. S. Shin, S. Y. Min, and Y. G. Lim. 2002. Streptomyces with antifungal activity against rice blast causing fungus, Magnaporthe grisea. J. Microbiol. Biotechnol. 12: 1026- 1028
  14. Lim, Y. S., K. C. Jung, S. H. Kim, and S. D. Bark. 1998. Crown rot of strawberry caused by Phytophthora cactorum. Kor. J. Plant Pathol. 14: 735-737
  15. Okuda, T., M. A. Klich, K. A. Seifert, and K. Ando. 2000. Media and incubation effects on morphological characteristics of Penicillium and Aspergillus, pp. 83- 99. In R. A. Samson, and J. I. Pitt (eds.), Integration of Modern Taxonomic Methods for Penicillium and Aspergillus Classification. Harwood Academic Publishers
  16. Peterson, S. W. 2000. Phylogenetic relationships in Aspergillus based on rDNA sequence analysis, pp. 323- 355. In R. A. Samson, and J. I. Pitt (eds.), Integration of Modern Taxonomic Methods for Penicillium and Aspergillus Classification. Harwood Academic Publishers
  17. Raper, K. B. and D. I. Fennell. 1973. The Genus Aspergillus, pp. 272- 273. Robert E. Krieger Publishing Company, NY, U.S.A
  18. Rhee, K. H. 2003. Purification and identification of an antifungal agent from Streptomyces sp. KH-614 antagonistic to rice blast fungus, Pyricularia oryzae, J. Microbiol. Biotechnol. 13: 984- 988
  19. Ryu, J. S., S. D. Lee, Y. H. Lee, S. T. Lee, D. K. Kim, S. J. Cho, S. R. Park, D. W. Bae, and K. H. Park. 2000. Screening and identification of an antifungal Pseudomonas sp. that suppresses balloon flower root rot caused by Rhizoctonia solani. J. Microbiol. Biotechnol. 10: 435- 440
  20. Shephard, M. C. 1987. Screening for fungicides. Annu. Rev. Phytopathol. 25: 189- 206 https://doi.org/10.1146/annurev.py.25.090187.001201
  21. Saitou, N. and M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecul. Biol. Evol. 4: 406- 425
  22. Varga, J., B. Toth, K. Rigo, J. Tererr, R. F. Hoekstra, and Z. Kozakiewicz. 2000. Phylogenetic analysis of Aspergillus section Circumdati based on sequences of the internal transcribed spacer regions and the 5.8S rRNA gene. Fungal Genet. Biol. 30: 71- 80 https://doi.org/10.1006/fgbi.2000.1204