Environmental Mutagens and Carcinogens (한국환경성돌연변이발암원학회지)

Korean Environmental Mutagen Society (한국환경성돌연변이발암원학회)
권호 표지

Effects of 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD) on Gene Expression in Mouse Skin Carcinogenesis

마우스 피부암 발생과정에 있어서 2,3,7,8-Tetrachlorodibenzo-p­Dioxin (TCDD) 처리에 의한 유전자발현 변화 연구

  • Ryeom Tai Kyung (Division of Genetic Toxicology, National Institute of Toxicological Research) ;
  • Kim Ok Hee (Division of Genetic Toxicology, National Institute of Toxicological Research) ;
  • Kong Mi Kyung (Division of Genetic Toxicology, National Institute of Toxicological Research) ;
  • Park Mi Sun (Division of Genetic Toxicology, National Institute of Toxicological Research) ;
  • Jee Seung Wan (Division of Genetic Toxicology, National Institute of Toxicological Research) ;
  • Eom Mi Ok (Division of Genetic Toxicology, National Institute of Toxicological Research) ;
  • Kang Ho Il (Division of Genetic Toxicology, National Institute of Toxicological Research)
  • Published : 2005.03.01
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Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) displays high toxicity in animals and has been implicated in human carcinogenesis. Although the mechanism of carcinogenesis by TCDD is unclear, it is considered to be a non-genotoxic compound and tumor promoter. In our experiment, we investigated the effects of TCDD on gene expression in mouse skin carcinogenesis. We used cDNA microarray to detect the differential gene expression in tumors induced in hairless mouse skin by MNNG plus TCDD protocol. We found that erb-2, c-ets2 and p27$^{kip1}$ were significantly up-regulated, but TNFR2, AKT-l, integrin $\beta$l, maspin, IGF-l, c-raf-l, Rb were significantly down-regulated, in tumor region, respectively. We also found that the expression of 53 genes involved in cen cycle, signal transduction, apoptosis, adhesion molecule, angiogenesis, and invasion, were changed two fold more, in tumor surrounding region. These data suggest that TCDD alters the expression of a large array of genes involved in apoptosis, cytokine production and angiogenesis in mouse skin carcinogenesis.

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