한국환경성돌연변이발암원학회지 (Environmental Mutagens and Carcinogens)
- 제25권1호
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- Pages.6-12
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- 2005
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- 1225-6307(pISSN)
Photo-Ames Assay를 이용한 광발암성 예측
Prediction of Photo-Carcinogenicity from Photo-Ames Assay
- Hong Mi Young (Korea Institute of Toxicology, Korea Research Institute of Chemical Technology) ;
- Kim Ji Young (Korea Institute of Toxicology, Korea Research Institute of Chemical Technology) ;
- Chung Moon Koo (Korea Institute of Toxicology, Korea Research Institute of Chemical Technology) ;
- Lee Michael (Korea Institute of Toxicology, Korea Research Institute of Chemical Technology)
- 발행 : 2005.03.01
초록
Many compounds might become activated after absorption of UV light energy. In some cases, the resulting molecule may undergo further biological reaction of toxicological relevance related especially to the photo-carcinogenicity resulting from photo-genotoxicity. However, no regulatory requirements have been issued with the exception of guideline issued by the Scientific Committee of Cosmetology, Commission of the European Communities (SCC/EEC) on the testing of sunscreens for their photo-genotoxicity. Thus, the objectives of this study are to investigate the utility of photo-Ames assay for detecting photo-mutagens, and to evaluate its ability to predict rodent photo-carcinogenicity. Photo-Ames assay was performed on five test substances that demonstrated positive results in photo-carcinogenicity tests: 8-methoxypsoralen (photoactive substance that forms DNA adducts in the presence of ultraviolet A irradiation), chlorpromazine (an aliphatic phenothiazine an a-adr-energic blocking agent), lomefloxacin (an antibiotic in a class of drugs called fluoroquinolones), anthracene (a tricyclic aromatic hydrocarbon a basic substance for production of anthraquinone, dyes, pigments, insecticides, wood preservatives and coating materials) and retinoic acid (a retinoid compound closely related to vitamin A). Out of 5 test substances, 3 showed a positive outcome in photo-Ames assay. With this limited data set, an investigation into the predictive value of this photo-Ames test for determining the photo-carcinogenicity showed that photo-Ames assay has relatively low sensitivity (the ability of a test to predict carcinogenicity). Thus, to determine the use of in vitro genotoxicity tests for prediction of carcinogenicity,' several standard photo-genotoxicity assays should be compared for their suitability in detecting photo-genotoxic compounds.
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