Archives of Pharmacal Research

  • 제28권3호
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  • Pages.305-310
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  • 2005
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  • 0253-6269(pISSN)
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  • 1976-3786(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

Agastache rugosa Leaf Extract Inhibits the iNOS Expression in ROS 17/2.8 Cells Activated with TNF-$\alpha$ and IL-$\beta$

  • Oh Hwa Min (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University) ;
  • Kang Young Jin (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University) ;
  • Kim Sun Hee (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University) ;
  • Lee Young Soo (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University) ;
  • Park Min Kyu (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University) ;
  • Heo Ja Myung (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University) ;
  • Sun Jin Ji (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University) ;
  • Kim Hyo Jung (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University) ;
  • Kang Eun Sil (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University) ;
  • Kim Hye Jung (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University) ;
  • Sea Han Geuk (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University) ;
  • Lee Jae Heun (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University) ;
  • YunChoi Hye Sook (Natural Products Research Institute, Seoul National University)
  • 발행 : 2005.03.01
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초록

It has been suggested that nitric oxide (NO) derived from inducible nitric oxide synthase (iNOS) may act as a mediator of cytokine-induced effects on bone turn-over. NO is also recognized as an important factor in bone remodeling, i.e., participating in osteoblast apoptosis in an arthritic joint. The components of Agastache rugosa are known to have many pharmacological activities. In the present study, we investigated the effects of Agastache rugosa leaf extract (ELAR) on NO production and the iNOS expression in ROS 17/2.8 cells activated by a mixture of inflammatory cytokines including TNF-$alpha$ and IL-1$\beta$. A preincubation with ELAR significantly and concentration-dependently reduced the expression of iNOS protein in ROS 17/2.8 cells activated with the cytokine mixture. Consequently, the NO production was also significantly reduced by ELAR with an IC$_{50}$ of 0.75 mg/mL. The inhibitory mechanism of iNOS induction by ELAR prevented the activation and translocation of NF-$\kappa$B (p65) to the nucleus from the cytosol fraction. Furthermore, ELAR concentration-dependently reduced the cellular toxicity induced by sodium nitroprusside, an NO-donor. These results suggest that ELAR may be beneficial in NO-mediated inflammatory conditions such as osteoporosis.

키워드

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