Journal of Korean society of Dental Hygiene (한국치위생학회지)
- Volume 4 Issue 2
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- Pages.153-163
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- 2004
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- 2287-1705(pISSN)
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- 2288-2294(eISSN)
Isolation and identification of Lactobacillus inhibiting the production of halitosis by anaerobic bacteria
구취를 유발하는 혐기성 세균의 증식을 억제하는 유산 간균의 분리 및 동정
- Kim, Mi-Hyung (Department of Dental Hygiene, Gwang-Ju Health College) ;
- Kim, Seon-Mi (College of Dentistry, Chonnam National University)
- Published : 2004.12.30
Abstract
There are normal inhabitants doing medically useful functions in the body. There are many kinds of bacteria performing specific functions in the oral cavity. Two strains of lactic acid bacteria were isolated from normal inhabitants of children 's oral cavity, which inhibited the the production of halitosis by anaerobic bacteria. The authors identified the isolates by the lest using API 50 CHL medium kit. 1. Two isolates were Gram-positive bacilli and produced hydrogen peroxide. 2. The optical density was 1.286 in the supernatant of Fusobacterium nucleatum after vortexing for 30 minutes, whereas in the supernatant of combined Fusobacterium nucleatum and each isolate, they were reduced to 0.628 and 0.497, which the percentages of coaggregation between them were 29.4% and 57.8%, respectively. 3. The optical density of Fusobacterium nucleatum precipitate was 1.794 in the culture media containing cysteine and