한국군사과학기술학회지 (Journal of the Korea Institute of Military Science and Technology)

  • 제7권3호
  • /
  • Pages.110-121
  • /
  • 2004
  • /
  • 1598-9127(pISSN)
  • /
  • 2636-0640(eISSN)
한국군사과학기술학회 (The Korea Institute of Military Science and Technology)
권호 표지

열분해 질량분석법을 이용한 생물학 물질의 특성 연구

Characteristics study of biological materials using pyrolysis-mass spectrometry

  • 발행 : 2004.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Pyrolysis-mass spectrometry, incorporating an in situ thermal hydrolysis and methylation(THM) step, has been used to study biological materials for bacteria, toxin and virus. Newly developed pyrolyzer was used to decompose biological materials, and tetramethylammonium hydroxide(TMAH) was used as a methylation reagent. Chemical ionization(CI) using ethanol and ion trap mass spectrometer(ITMS) were used to ionize and analyze of pyrolysis components, respectively. Analytical characteristics of bacteria (including spore), virus and toxin were analyzed. Also acquisition and interpretation of mass spectra as biomarkers for classification/identification of biological material s were explained.

키워드

참고문헌

  1. W. H. Griest, M. B. Wise, K. J. Hart, S. A. Lammert and C. V. Thompson, 'Biological agent detection and identification by the Block II chemical biological mass spectrometer', Field Anal Chern Tech, vol. 5(4), 2001, pp.177-184
  2. S. Luo, J. Mohr, D. Sickenberger and A. Hryncewich, 'Study of purified bacteria and viruses by pyrolysis mass spectrometry', Field Anal Chern Tech, vol. 3(6), 1999, pp.357-374
  3. M. Xu and K. J. Voorhees, 'Differentiation and classification of user-specified bacterial groups by in situ thermal hydrolysis and methylation of whole bacterial cells with tert-butyl bromide chemical ionization ion trap mass spectrometry', Analytica Chimica Acta, vol. 418, 2000, pp.119-128
  4. A. J. Madonna, K. J. Voorhees and T. L. Hadfield, 'Rapid detection of taxonomically important fatty acid methyl ester and steroid biomarkers using in situ thermal hydrolysis /rnethylation mass spectrometry (THM-MS): implications for bioaerosol detection', J. Anal. Appl, Pyrolysis, vol. 61, 2001, pp.65-89
  5. M. Xu, K. J. Voorhees and T. L. Hadfield, 'Repeatability and pattern recognition of bacterial fatty acid profiles generated by direct mass spectrometric analysis of in situ thermal hydrolysis/methylation of whole cells', Talanta, vol. 59, 2003, pp.577- 589
  6. J. D. J. van den Berg and J. J. Boon, 'Unwanted alkylation during direct methylation of fatty (di)acids using tetramethyl ammonium hydroxide reagent in a Curie-point pyrolysis unit', J. Anal. Appl. Pyrolysis, vol. 61, 2001, pp.45-63
  7. S. A. Barshick, D. A. Wolf and A. A. Vass, 'Differentiation of microorganisms based on pyrolysis-ion trap mass spectrometry using chemical ionization', Anal Chern, vol. 71, 1999, pp.633~641
  8. K. J. Hart, M. B. Wise, W. H. Griest and S. A. Lammert, 'Design, development, and performance of a fieldable chemical and biological agent detector', Field Anal Chern Tech, vol. 4(2-3), 2000, pp.93-110
  9. C. D. Havey, F. Basile, C. Mowry and K. J. Voorhees, 'Evaluation of a micro-fabricated pyrolyzer for the detection of Bacillus anthracis spores', ]. Anal. Appl. Pyrolysis, vol. 72, 2004, pp.55-61
  10. M. B. Beverly, K. ]. Voorhees and T. L. Hadfield, 'Direct mass spectrometric analysis of Bacillus spores', Rapid Comm. Mass Spectrom., vol. 13, 1999, pp.2320 - 2326
  11. F. Basile, M. B. Beverly and Kent J. Voorhees, 'Pathogenic bacteria: their detection and differentiation by rapid lipid profiling with pyrolysis mass spectrometry', trends in Analytical Chemistry, vol. 17(2), 1998, pp.95-109
  12. A. D. Hendricker and Kent J. Voorhees, 'Amino acid and oligopeptide analysis using Curie-point pyrolysis mass spectrometry with in-situ thermal hydrolysis and methylation: mechanistic considerations', J. Anal. Appl, Pyrolysis vol. 48, 1998, pp.I7~33
  13. A. J. Madonna, K. J. Voorhees, T. L. Hadfield and E. J. Hilyard, 'Investigation of cell culture media infected with viruses by pyrolysis mass spectrometry: implications for bioaerosol detection', J Am Soc Mass Spectrom, vol. 10, 1999, pp.502-511
  14. F. Basile, M. B. Beverly, C. Abbas-Hawks, C. D. Mowry, K. J. Voorhees and T. L. Hadfield, 'Direct mass spectrometric analysis of in situ thermally hydrolyzed and methylated lipids from whole bacterial cells', Anal Chern, vol. 70, 1998, pp.1555-1562