Factors Influencing Airborne Concentration of Fungi, Bacteria and Gram Negative Bacteria in Kindergarten Classroom

유치원 교실에서 공기 중 박테리아와 곰팡이 발생에 영향을 미치는 요인

  • Published : 2004.12.01

Abstract

Airborne bacteria, gram negative bacteria (GNB) and fungi were measured in 70 class of 17 kindergartens. The objective of this study is to identify the factors influencing airborne concentrations of bacteria, GNB and fungi using multiple regression analysis. The average concentrations of bacteria and fungi exceeded $1,000\;CFU/m^3$. The average of GNB was $3.7{\times}10^2\;CFU/m^3$. This results indicated that air of kindergartens was contaminated with microbes such as bacteria and fungi. ANOVA test found that the concentrations of bacteria, GNB and fungi were significantly different by the characteristics of weather (rain, after rain, sunny) sampling date (July, August, September and October), the location of sampling site (ground level and basement) and the location of toilet (inside class, nearby class and away class). Multiple regression tests concluded that sampling date, the scale of city where kindergartens are located, the location of sampling site and ventilation efficiency can significantly affect the airborne concentration of bacteria, GNB and fungi. Most of these factors could be related moisture. Environmental factors that can cause the increment of moisture should be controlled in order to reduce airborne concentration of bacteria, GNB and fungi. Legal actions concerning prohibition on the presence of toilet inside class and ventilation criteria should be taken.

Keywords

References

  1. 교육인적자원부. 유아교육진흥법시행령. 일부개정 2001. 1, 29 대통령령 제 17115호, 2001
  2. 보건복지부. 영유아보육법. 보육시설의 시설기준 등[제 8조] 일부개정 2004. 3. 11 법률 제 07186호, 2004
  3. 보건복지부. 영유아보육법시행규칙. 보육시설의 시설기준[제 3조, 7조, 12조 관련], 별표 1, 2, 5. 일부개정 2000. 8. 18 제 169호, 2000
  4. 교육인적자원부. 유아교육법. 유치원의 설립 등 [제 8조]. 제정 2004. 1 29 법률 제 07120호, 2004
  5. 천식 및 알레르기 예방본부. 질병정보 및 언론보도.2004. Available from URL:http://www.wadpo.or.kr
  6. American Conference of Governmental Industrial Hygienists (ACGIH) : Bioaerosols assessment and control. ACGIH, 1999
  7. American Industrial Hygiene Association (AIHA) : Field guide for the determination of biological contaminants in environmental samples. AIHA PRESS, 1996
  8. Arturo, B., Gwen, W., Alan, M. D., Catherine, L. G. and Cristina, L. H. et al. : School-based identification of asthma in a low-income population. Pediatric Pulmon, 30(4), 297-301, 2000 https://doi.org/10.1002/1099-0496(200010)30:4<297::AID-PPUL4>3.0.CO;2-#
  9. Chapman, M. D., Aalberse, R. C., Brown, M. J. and Platts-Mills, T. A. : Monoclonal antibodies to feline allergen Fel d I, N terminal sequence analysis, and development of a sensitive two-site immunoassay to assess Fel d I exposure. J. Immunol., 140, 812-818, 1998
  10. Dorneals, D. A. A., Charpin, D. and Birnbaum, J. et al. : Indoor allergen levels in day nurseries. J. Allergy Clin Immunol., 95, 1158-1163, 1995 https://doi.org/10.1016/S0091-6749(95)70071-4
  11. Dybendal, T. and Elsayed, S. : Dust from carpeted and smooth floors. V. Cat (Fel d f) and mite (Der p I and Der f I) allergen levels in school dust. Demonstration of the basophil histamine release induced by dust from classrooms. Clin. Exp. Allergy, 22, 1100-1106, 1992. https://doi.org/10.1111/j.1365-2222.1992.tb00136.x
  12. Dybendal, T and Elsayed, S. : Dust from carpeted and smooth floors. Allergy in homes compared with those in schools in Norway. Allergy, 49, 210-216, 1994. https://doi.org/10.1111/j.1398-9995.1994.tb02651.x
  13. Ehnert, B., Lau-Schadendorf, S., Weber, A., Buettner, P. and Schou, C. et al. : Reducing domestic exposure to dust mite allergen rdeuces bronchial hyperreactivity in sensitivity chileren with asthma. J. Allergy Clin. Immunol., 90, 135-138, 1992 https://doi.org/10.1016/S0091-6749(06)80024-2
  14. Hyvarinen, A., Meklin, T., Vepsalainen, A., Nevalainen : Fungi and actinobacteria in moisture-damaged building materials-concentrations and diversity. International Biode and Biode., 49, 27-37, 2002 https://doi.org/10.1016/S0964-8305(01)00103-2
  15. Kampen V. van, Merget R. and Baur X. : Occupational airway sensitizers: an overview on the respective literature. American J. of Industrial Med., 38(2), 164-218, 2000 https://doi.org/10.1002/1097-0274(200008)38:2<164::AID-AJIM7>3.0.CO;2-2
  16. Kramer, D., Heinrich, J., Wjst, M. and Wichmann, H.E. : Age of entry to day nursery and allergy in alter childhood. Lancert, 353, 450-454, 1999 https://doi.org/10.1016/S0140-6736(98)06329-6
  17. Manuel, E. S., Edwin, K. S., Lars, A, H., Scott, T. W. and Juan, C. C. : Material history, sensitization to allergens, and current wheezing, rhinitis, and eczema among children in Costa Rica. Pediatric Pulmon., 33(4), 237-243, 2002 https://doi.org/10.1002/ppul.10070
  18. Miller, J. D., Hausley, P. D. and Reinhardt, J. H. : Air sampling results in relation to extent of fungal colonization of building materials in some water-damaged buildings. Indoor Air, 10, 146-151,2000. https://doi.org/10.1034/j.1600-0668.2000.010003146.x
  19. Munir, A. K., Einarsson, R. and Dreborg, S. : Allergen avoidance in a daycare center. Allergy, 51, 36-41, 1996. https://doi.org/10.1111/j.1398-9995.1996.tb04547.x
  20. Munir, A. K. M., Einarsson, R., Schou, C. and Dreborg, S. K. G. : Allergens in school dust I. The amount of the major cat (FeI d I) and dog (Can f I) allergens in dust from Swedish schools is high enough to probably cause perennials symptoms in most children with asthma who are sensitized to cat and dog. J. Allergy Clin. Immunol., 91, 1067-1074, 1993 https://doi.org/10.1016/0091-6749(93)90221-Z
  21. Nelson, R. P., DiNicolo, R. and FemandezCaldas E. et al. : Allergen-specific IgE levels and mite allergen exposure in children with acute asthma first seen in an emergency department and in nonasthmatic control subjects. J. Allergy Clin. Immunol., 98, 258-263, 1996 https://doi.org/10.1016/S0091-6749(96)70148-3
  22. Occupational Safety and Health Administration. OSHA Technical manual, section III: Chapter 2, Indoor air quality, Available from DRL:http://www.osha-slc.gov/ dts/otm
  23. Persky, V. W., Slezak, J. and Contreras, A. et al. : Relationships of race and socioeconomic status with prevalence, severity, and symptoms of asthma in Chicago school children. Ann. Allergy Asthma Immunol., 81, 266-271, 1998 https://doi.org/10.1016/S1081-1206(10)62824-4
  24. Perzanowski, M. S., Ronmark, E. and Nold, B. et al. : Relevance of allergens from cat and dogs to asthma in the northernmost province of Sweden. schools as a major site of exposure. J. Allergy Clin. Immunol., 103, 1018-1024, 1999 https://doi.org/10.1016/S0091-6749(99)70173-9
  25. Peter, J. : Helms. Issues and unmet needs in pediatric asthma. Pediatric Pulmon., 30(2), 159-165, 2000 https://doi.org/10.1002/1099-0496(200008)30:2<159::AID-PPUL14>3.0.CO;2-G
  26. Rautiala, S., Reponen, T., Hyvarinen, A., Nevalainen, A. and Husman, T. et ai. : Exposure to airborne microbes during the repair of moldy buildings. Ame. Ind. Hyg. Associ., 57, 279-284, 1996 https://doi.org/10.1080/15428119691015016
  27. Reponen, T. ; Bioaerosol and particle mass levels and ventilation in Finish homes. Environ. Int., 15, 203-208, 1989 https://doi.org/10.1016/0160-4120(89)90028-7
  28. Reponen, T., Hyvarinen, A., Ruuskanen, J., Raunemaa, T. and Nevalainen, A. : Composition of concentrations and size distributions of fungal spores in buildings with and without mould problems. J. Aerosol. Sci., 25(8), 595-1603, 1994 https://doi.org/10.1016/0021-8502(94)90074-4
  29. Sarpong, S. B., Wood, R. A., Karrison, T. and Eggleston, P. A. : Cockroach allergen(Bla g I)in school dust. Js. Allergy Clin. Immunol., 99, 486-492, 1997 https://doi.org/10.1016/S0091-6749(97)70074-5
  30. Shapiro, G. G. and Stout, J. W. : Childhood asthma in the United States: Urban issues. Pediatric Pulmon., 33(1), 47-55, 2002 https://doi.org/10.1002/ppul.10029
  31. Stephen, H. L. H., Ting-Fan, L., Gladys, H., Eric, W. and Albert, M. L. et al. : Evaluation of an asthma management program for Chinese children with mild-to-moderate asthma in Hong Kong. Pediatric Pulmon, 33(1), 22-29, 2002 https://doi.org/10.1002/ppul.10024
  32. The Burden of ASTHMA 2004 May 4. WAD Activity Organizer. 2004. Available from : http://207.159. 65.33/ wadsetup/
  33. Teeuw, K. B., Vandenbroucke-Grauls, C. M. J. E. and Verhoef, J. : Airborne gram negative bacteria and endotoxin in sick building syndrome: a study in Dutch government office buildings. Arch. Intern. Med., 154, 2339-2345, 1994 https://doi.org/10.1001/archinte.154.20.2339
  34. Warner, A. M., Bjorksten, B. and Munir, A. K. et al. : Childhood asthma and exposure to indoor allergens: low mite levels are associated with sensitivity. Pediatr Allergy Imm., 7, 61-67, 1996 https://doi.org/10.1111/j.1399-3038.1996.tb00108.x
  35. Yang, C. Y., Lin, M. C. and Hwang, K. C. : Childhood asthma and the indoor environment in a subtropical area. Chest., 114, 393-397, 1998 https://doi.org/10.1378/chest.114.2.393
  36. Zock, J. P. and Brunekreef, B. : House dust mite allergen levels in dust from schools with smooth and carpeted classroom floors. Clin. Exp. Allergy, 25, 549-553, 1995 https://doi.org/10.1111/j.1365-2222.1995.tb01093.x