KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
권호 표지

Improvement of Regeneration Efficiency from Mature Embryo and Leaf Base Segment in Korean Oat Genotypes

  • Kim Kyung-Hee (Department of Plant Resources, Dongguk University) ;
  • Moon Jung-Hun (Department of Plant Resources, Dongguk University) ;
  • Lee Sang-Kyu (Department of Plant Resources, Dongguk University) ;
  • Lee Byung-Moo (Department of Plant Resources, Dongguk University)
  • Published : 2004.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Mature embryo and leaf base segment of Korean oat were used as materials in an experiment to check plant regeneration efficiency. MS media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin, and picloram were used for callus induction from mature embryos and leaf base segments. Three mg/l of 2,4­D and 3 mg/l of picloram in callus induction medium showed high frequency for plant regeneration from mature embryos. Leaf base segments were transferred to callus induction medium and incubated at $25^{\circ}C$ in 16/8 hr light/dark cycle for 3 weeks. Callus induction from leaf base segments of Malgwiri showed high efficiency in medium containing 3 mg/l of 2,4-D and 1 mg/l of kinetin $(91.8\%)$. In case of Samhangwiri, the combinations of phytohormones did not show significant difference. Regeneration from leaf base segments showed high frequency in shoot medium containing 1 mg/l of antiauxin, tri-iodobenzoic acid (TIBA) and 1 mg/l of 6-benzyladenine (BA). Calli induced from leaf base segments of Samhangwiri and Malgwiri in media containing 3 mg/l of 2,4-D and 3 mg/l of picloram showed high regeneration frequency. It appears that the callus initiation medium may be an important factor for subsequent plant regeneration.

Keywords

References

  1. Barro, F, M E Cannell, P A Lazzeri, and P Barcelo 1998 The influence of auxin on transformation of wheat and tritordeum and analysis of transgene integration patterns in transformants Theor Appl Genet. 97 : 684-695 https://doi.org/10.1007/s001220050944
  2. Birsin, M A, S. Onde, and M Ozgen 2001 Callus induction and plant regeneratIon from mature embryos of oat (Avena sativa). Turk. Biol. 25.427-434
  3. Bregitzer, P, D. A. Somers, and H. W. Rines, 1989. Development and characterization of friable, embryogenic oat callus Crop Sci 29. 798-803 https://doi.org/10.2135/cropsci1989.0011183X002900030052x
  4. Bregitzer, P, W R. Bushnell, H. W Rines, and D A Somers 1991. Callus formation and plant regeneration from somatic embryos of oat (Avena sativa) Plant cell Rep 10 243-246
  5. Buter, B. 1997 In vitro haploid production in maize In S. M. Jam, S K Sopory, and R E Veilleux (eds) In vitro haploid production in higher plants, vol 4 Kluwer, Dordreckt, The Netherlands, pp 37-71
  6. Carter, O., Y. Yamada, and E Takahashi. 1967. Tissue culture of oats Nature 214 1029-1030 https://doi.org/10.1038/2141029a0
  7. Chen, H., G Xu, D. C Loschke, L Tomaska, and B G Rolfe 1995 Efficient callus formation and plant regeneratioon from leaves of oats (Avena sativa) Plant Cell Rep. 14: 393-397
  8. Chen, Z , Q Zhuge, and C. Sundqvist 1995. Oat leaf base: tissue with an efficient regeneration capacity Plant Cell Rep 14 354-358
  9. Choi, Y E., H S KIm, W Y Sho, and D. C Yang. 1997. Development and structural aspects of somatic embryos formed on medium containing 2,3,5-triiodobenzoic acid Plant Cell Rep. 16.738-744 https://doi.org/10.1007/s002990050312
  10. Chugh, A. and P Khurana 2003 Regeneration via somatic embryogenesis from leaf basal segments and genetic transformation of bread and emmer wheat by particle bombardment Plant Cell Tissue Organ Cult 74 151-161 https://doi.org/10.1023/A:1023945610740
  11. Cummings, D P, C E Green, and D D Stuthman 1976 Callus induction and plant regeneration in oats Crop Sci 16 465-470 https://doi.org/10.2135/cropsci1976.0011183X001600040006x
  12. Dudits, D , L Bogre, and J Gyorgyey 1991 Molecular and cellular approaches to the analysis of plant embryo development from somatic cells in vitro J. Cell Sci 99 475-484
  13. Gless, C., H Lorz, and A Jahne-Gartner 1998a. Establishment of a highly efficient regeneratIon in oats Crop Sci 16 465-470 https://doi.org/10.2135/cropsci1976.0011183X001600040006x
  14. Gless, C., H. Lorz, and A. Jahne-Gartner 1998b Transgenic oat plants obtained at high efficiency by microprojectile bombardment of leaf base segments J Plant Physiol 152 151-157 https://doi.org/10.1016/S0176-1617(98)80126-0
  15. Gless, C, H. Lorz, and A. Jahne-Gartner 1998c. Establishment of a highly efficient regeneration system from leaf base segments of oat (Avena sativa L ). Plant Cell Rep 17: 441-445 https://doi.org/10.1007/s002990050422
  16. Heyser, J W and M W Nabors 1982 Long term plant regeneration, somatic embryogenesis, and green spot formation in secondary oat (Avena sativa) callus Z Pflanzenphysiol 107. 153-160 https://doi.org/10.1016/S0044-328X(82)80101-3
  17. Jahne-Gartner, A., D Becker, and H Lorz 1995. Genetic engineering of cereal crop plants a review Euphytica 85 . 35-44 https://doi.org/10.1007/BF00023928
  18. Jahne-Gartner, A. and H Lorz 1996. In vitro regeneration systems of barley (Hordeum vulgare L.). Plant Tissue Cult. Biotechol 2 11-23
  19. Kim, K H. and B M. Lee. 2002. Callus induction and plant regeneration from mature embryos in oat Korean J. Crop Sci.47(5): 352-355
  20. Kiviharju, E , M Puolimatka, M. Saastamonien, S. Hovinen, and E. Pehu 1998 The effect of genotype on anther culture response of cultivated and wild oats Agri Food Sci. Finl 7 409-422
  21. Kiviharju, E., M. Puolimatka, M Saastamonien.and E Pehu 2000 Extension of anther culture to several genotypes of cultivated oats Plant Cell Rep. 19 674-679 https://doi.org/10.1007/s002999900165
  22. Kiviharju, E and A Tauriainen 1999 2,4-dichlorophenoxyacetic acid and kinetin in anther culture of cultivated and wild oats and their interspecific crosses; plant regeneration from Avena sativa L. Plant Cell Rep 18 582-588 https://doi.org/10.1007/s002990050626
  23. Liu, C. M., Z. H. Xu, and N H Chua 1993. Auxin polar transport is essential for the establishment of bilateral symmetry during early plant embryogenesis Plant Cell 5 . 621-630 https://doi.org/10.1105/tpc.5.6.621
  24. Lorz, H, C T. Harms, and I Potrykus 1976. Regeneration of plants from callus in Avena sativa. L Z Pflanzenzuecht 77 257-259
  25. Murashige, T. and Skoog, F 1962 A revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol Plant 15 .473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  26. Nabors, M W, C. S Kroskey, and D W Mchugh 1982 Green spots are predictors of high callus growth rates and shoot formation in normal and salt stressed tissue cultures of oat (Avena sativa L) Z Pflanzenzuecht 105. 341-349
  27. Nuutila, A. M., C Villiger, and K -M Okasman-Caldentey 2002 Embryogenesis and regeneration of green plantlets from oat(Avena sativa L) leaf-base segments: influence of nitrogen balance, sugar and auxin. Plant Cell Rep 20 1156-1161 https://doi.org/10.1007/s00299-002-0467-7
  28. Rines, H. W 1983 Oat anther culture: genotype effects or callus initiation and production of a haploid plant. Crop Sci 23. 268-272
  29. Rines, H W and H H Luke. 1985. Selection and regeneration of toxin-in-sensitive plants from tissue cultures of oats (Avena sativa) susceptible to Helminthosporium victoriae Theor. Appl. Genet. 71 . 16-21 https://doi.org/10.1007/BF00278247
  30. Rines, H W and T J. McCoy. 1981 Tissue culture initiation and plant regeneration in hexaploid species of oats Crop Sci 21 . 837-842 https://doi.org/10.2135/cropsci1981.0011183X002100060010x
  31. Somers, D A, H W Rines, W. Gu, H F Kaeppler, and W R Bushnell. 1992. Fertile, transgenic oat plants Biotechology 10 . 1589-1594
  32. Tobert, K A, H. W. Rines, and D A Somers. 1998a Transformation of oat using mature embryo-derived tissue cultures Crop Sci. 38 : 226-231 https://doi.org/10.2135/cropsci1998.0011183X003800010038x
  33. Tobert, K. A , H W. Rines, H F Kaeppler, G K. Menon, and D A Somers 1998b. Genetically engineering elite oat cultivars Crop Sci 38. 1685-1687 https://doi.org/10.2135/cropsci1998.0011183X003800060044x
  34. Villemont, E , F Dubois,R S Sangwan, G Vasseur, Y Bourgeois, and B S Sangwan-Norreel 1997 Role of the host cell cycle in the Agrobacterium-mediated genetic transformation of Petnia : evidence of an S-phase control mechanism for T-DNA transfer Planta 201 : 160-172 https://doi.org/10.1007/BF01007700
  35. Wernicke, W and L. Milkowitz. 1987 Effect of auxin on the mitotic cell cycle in cultured leaf segments at different stages of development in wheat. Physiol Plant 69. 16-22 https://doi.org/10.1111/j.1399-3054.1987.tb01940.x
  36. Zhang, S , H. Zhong, and M. B Sticklen. 1996 Production of multiple shoots from shoot apical meristem of oat (Avena sattva L.) J Plant physiol 148 667-671 https://doi.org/10.1016/S0176-1617(96)80365-8