Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Characterization of Vibrio vulnificus Isolated from Domestic Coastal Area

국내 연안에서 분리한 Vibrio vulnificus의 특성

  • Park Geun-Tae (Faculty of Marine Science, Cheju National University) ;
  • Park Min Jung (Laboratory of Enteric Infections, Department of Microbiology, NIH) ;
  • Jung Cho-Rok (Lab. of Cellular Biology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Song Choon-Bok (Faculty of Marine Science, Cheju National University) ;
  • Lee Jehee (Faculty of Marine Science, Cheju National University) ;
  • Yeo In-Kyu (Faculty of Marine Science, Cheju National University) ;
  • Jeon You Jin (Faculty of Marine Science, Cheju National University) ;
  • Heo Moon-Soo (Faculty of Marine Science, Cheju National University)
  • Published : 2004.12.01
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Abstract

Five strains of Vibrio vulnificus, which cause serious septicemia in human worldwidely, was isolated from marine environments of Korea costal area from May to July of 2002. The isolated strains were identified by API 20E kit and partial 16S rDNA sequence analysis. 16S rDNA sequence of the isolates showed $99\%$ similarity with V. vulnificus ATCC 27562. The proteins of V. vulnificus isolates were examined by analyzing patterns of the cell lysates and outer membrane proteins (OMP). The OMP separated from cell lysates showed the common protein band. Therefore OMP profiles might be useful for the identification of V. vulnificus sp.

2002년 5월에서 7월사이 한국 근해로부터 총 가검물 50례 중에서 V. vulnificus은 5균주가 분리되었고, API 20E kit의동정률이 $89.4\~93.7\%$로 나타났다. V. vulnificus 16S rDNA를 증폭하여 얻은 산물을 cloning하여 염기서열을 결정하고 분석한 결과 V. vulnificus로 확인되었다. 동정된 분리균주들의 cell lysates을 SDS-PAGE로 분석하였고 표준균주로 사용된 V. vulnificus ATCC 27562와 분리 균주들을 비교해 본 결과 단백밴드pattern이 많은 차이를 보였으나 외막단백질은 V. vulnificus간에는 공통의 밴드가 확인되었으나 V. parahaemolyticus 와는 차이를 보였다.

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References

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