생명과학회지 (Journal of Life Science)

  • 제14권6호
  • /
  • Pages.938-950
  • /
  • 2004
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

미토콘드리아 12S 리보종 RNA 유전자배열에 의한 한국해역 멸치 개체군의 유전자 구조

Population Genetic Structure of Japanese Anchovy (Engraulis japonicus) in Korean waters Based on Mitochondrial 12S Ribosomal RNA Gene Sequences

  • 김진영 (국립수산과학원 남해수산연구소) ;
  • 조은섭 (국립수산과학원 남해수산연구소) ;
  • 김우진 (국립수산과학원 생명공학연구단)
  • 발행 : 2004.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

한국해역에 분포하는 멸치의 유전학적 특성을 연구하기 위하여 미토콘드리아 12S 리보좀 RNA 유전자배열 (339 bp)을 분석하였다. 황해남부, 제주연안, 남해동부 등 3지역의 시료로 부터 총 35 mtDNA의 haplotype을 구하였다. 황해남부에서 채집된 멸치의 AN8T103은 PAUP 분석에서 $0.2-4.1\%$로 분리되는 독립적인 계통을 보이므로서 다른 연구해역으로부터 유입된 개체군인 것으로 보이나 금후 추가연구가 필요하다. AN8T103을 제외한 유전자 다양성은 $0.3-3.8\%$로서 개체군 내 염기다양성은 0.015(황해), 0.013(제주도), 0.015(남해)로 나타났다. 암컷유전자이동은 상당히 높았으며(Nm=25.5-36.44), 지역간 유전자거리(FST)는 유의한 차를 보이지 않았다$(P>0.01)$. 이러한 결과는 한국해역에 서식하는 멸치가 지리적으로 무작위 분산된 개체군임을 암시한다.

We used portions of mitochondrial 125 ribosomal RNA gene sequences (339 bp) to investigate the phylogenetic and population genetic characteristics of the Japanese anchovy, Engraulis japonicus, in Korean waters. A total of 35 mtDNA haplotypes were obtained from the samples collected in 3 locations (the southern area of the Yellow Sea, the western coast of Jejudo, and the eastern area of the South Sea) in Korean waters. One haplotype, AN8T103, obtained from the southern area of the Yellow Sea, was formed according to an independent phylogenetic individual in the PAUP analysis, which was separated from the others by a $0.2-4.1\%$ sequence divergence. This distinct haplotype appeared to be one that was carried by immigrants from another study area, but further study is necessary. Genetic divergence, except for AN8T103, was moderate to substantial $(0.2-3.8\%)$ and nucleotide diversity within populations was 0.015 for Yellow Sea, 0.013 for Jejudo, and 0.D15 for South Sea, respectively. The female gene flow was substantial or high (Nm=25.5-36.4), and the genetic distances between regions were not statistically significant $(P>0.01)$. These results indicated that the Japanese anchovy populations occurring in Korean waters were consisted of individuals randomly dispersed over geographic areas.

키워드

참고문헌

  1. Asahida, T., T. Kobayashi, K. Saitoh and I. Nakayama. 1996. Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea. Fish. Sci. 62, 727-730 https://doi.org/10.2331/suisan.62.727
  2. Avise, J. C. 1994. Molecular Markers, Natural History and Evolution. New York, 511 pp
  3. Bembo, D. G., G. R. Carvalho, M. Snow, N. Cingolani and T. J. Pitcher. 1995. Stock discrimination among European anchovies, Engraulis encrasicolus, by means of PCR- amplified mitochondria DNA analysis. Fish. Bull. 75, 31-40
  4. Bembo, D. G., G. R. Carvalho, N. Cingolani and T. J. Pitcher. 1996. Electrophoretic analysis of stock structure in Northern Mediterranean anchovies, Engraulis encrasicolus. ICES J. Mar. Sci. 53, 115-128 https://doi.org/10.1006/jmsc.1996.0011
  5. Borsa, P. 2002. Allozyme, mitochondria-DNA, and morphometric variability indicate cryptic species of anchovy (Engraulis encrasicolus). Biol. J. Linn. Soc. 75, 261-270
  6. Chiu, T. S., Y. J. Lee, S. W. Huang and H. T. Yu. 2002. Polymorphic microsatellite markers for stock identification in Japanese anchovy (Engraulis japonica). Mol. Ecol. Notes 2, 49-50 https://doi.org/10.1046/j.1471-8286.2002.00142.x
  7. Grant, W. S. 1985. Biochemical population genetics of the southern African anchovy, Engraulis capensis. J. Fish Bio. 27, 23-29 https://doi.org/10.1111/j.1095-8649.1985.tb04006.x
  8. Hellberg, M. E., R. S. Burton, J. E. Neigel and S. R. Palumbi. 2002. Genetic assessment of connectivity among marine populations. Bull. Mar. Sci. 70, 273-290
  9. Inoue, J. G., M. Miya, K. Tsukamoto and M. Nishida. 2001. Complete mitochondrial DNA sequence of the Japanese anchovy, Engraulis japonicus. Fish. Sci. 67, 828-835 https://doi.org/10.1046/j.1444-2906.2001.00329.x
  10. Kim, J. Y. 1983. Distribution of anchovy eggs and larvae off the western and southern coasts of Korea. J. Kor. Fish. Soc. 16, 401-409. (in Korean)
  11. Kim, J. Y. and Y. M. Choi. 1988. Vertical distribution of anchovy, Engraulis japonica eggs and larvae. J. Kor. Fish. Soc. 21, 139-144. (in Korean)
  12. Kimura, M.1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. J. Mol. Evol. 116, 111-120
  13. Lee, S. W. 1996. An outline of oceanophysics. Jipmundang Press, Seoul. 225pp
  14. Magoulas, A., N. Tsimenides and E. Zouros. 1996. Mitochondria DNA phylogeny and the reconstruction of population history of a species: the case of European anchovy (Engraulis encrasicolus). Mol. Biol. Evol. 13, 178-190 https://doi.org/10.1093/oxfordjournals.molbev.a025554
  15. Palumbi, S. R. 1994. Genetic divergence, reproductive isolation, and marine speciation. Ann. Rev. Ecol. Syst. 25, 547-572 https://doi.org/10.1146/annurev.es.25.110194.002555
  16. Schneider, S., D. Roessli and L. Exocofier. 2000. Arlequin: A software for Population Genetics Data Analysis, ver. 2.0, Genetics of Biometry Laboratory, Department of Anthropology, University of Geneva, Switzerland
  17. Sebastio, P., P. Zanelli and T. M. Neri. 2001. Identification of anchovy (Engraulis encrasicholus L.) and sardine (Sardinella aurita) by polymerase chain reaction sequence of their mitochondrial cytochrom b gene, and restriction analysis of polymerase chain reaction produce semipreserves. J. Agric. Food Chem. 49, 1194-1199 https://doi.org/10.1021/jf000875x
  18. Spanakis, E., N. Tsimenides and E. Zouros. 1989. Genetic differences between populations of sardine, Sardina pilchardus, and anchovy, Engraulis encrasicolus, in the Aegean and Ionina seas. J. Fish Biol. 35, 417-437 https://doi.org/10.1111/j.1095-8649.1989.tb02993.x
  19. Swofford, D. L. 2000. Phylogenetic Analysis Using Parsimony ($^*and$ other Methods). version 4.0. Sinauer Associates, Sunderland, Massachusetts
  20. Thomson, J. D., D. G. Higgins and T. J. Gibson. 1994. Clustal W: improving the sensitivity of progressive multiple sequences alignment through sequence weighting, position specific fap penalties and weight matrix choice. Nucl. Acid. Res. 22, 4673-4680 https://doi.org/10.1093/nar/22.22.4673
  21. Tudela, S., J. L. Garcia-Marin and C. Pla. 1999. Genetic structure of the European anchovy, Engraulis encrasicolus 1., in the north-west Mediterranean. J. Exp. Mar. Ecol. 234, 95-109 https://doi.org/10.1016/S0022-0981(98)00142-7
  22. Zhu, D., B. G. M. Jamieson, A. Hugall and C. Moritz. 1994. Sequence evolution and phylogenetic signal in control region and cytochrome b sequences of rainbowfishes (Melanotaeniidae). Mol. Biol. Evol. 11, 672-683

피인용 문헌

  1. DNA Analysis of mtDNA COI Gene in the Sharp-toothed Eel (Muraenesox cinereus Forskal) from Yeosu, Jinhae, Jeju, Goseoung, Jangheung and Haenam Populations in Korea Using PCR-aided RFLP vol.20, pp.4, 2011, https://doi.org/10.5322/JES.2011.20.4.551
  2. The Pulation Structure of the Pacific Cod (Gadus macrocephalus Tilesius) Based on Mitochondrial DNA Sequences vol.20, pp.3, 2010, https://doi.org/10.5352/JLS.2010.20.3.336
  3. Development of gene-associated single nucleotide polymorphisms for Japanese anchovy Engraulis japonicus through cross-species amplification vol.84, pp.1, 2018, https://doi.org/10.1007/s12562-017-1134-9