생명과학회지 (Journal of Life Science)

  • 제14권6호
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  • Pages.931-937
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  • 2004
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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밀양지역 들깨품종별 잎의 아미노산 조성 및 향기성분 비교

Comparative Studies on the Amino Acids and Flavor Compounds Among Some Varieties of Perilla Leaves Cultivated in Miryang Area

  • 발행 : 2004.12.01
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초록

우리나라 들깻잎의 주산지인 밀양지역에서 재배하고 있는 4종의 품질 특성을 조사하고자 아미노산과 휘발성 향 성분을 분석하여 비교하였다. 단백질구성아미노산에서 주요 아미노산은 aspartic acid, glutamic acid, glycine, alanine, arginine, 및 threonine 등 이었고, 그 외에 lysine, leucine, isoleucine 등도 다소 검출되었으나, tyrosine과 함황아미노산인 cysteine과 methionine의 함량이 가장 적 게 나타났으며, 유리 아미노산은 4종 모두에서 약34종이 검출되었다. 그리고 유리아미노산의 조성과 단백질구성아미노산의 조성에서 품종간의 차이는 거의 없는 것으로 나타났다. 들깻잎의 휘발성 향기성분을 GC-MSD로 분석한 결과, 잎들깨1호는 52종, 엽실들깨는 48종, 그리고 밀양9호와 YCPL은 47종이 분리되어 검출되었다. 깻잎의 휘발성 화합물중에서 가장 많은 함량으로 검출된 perilla ketone은 엽실들깨 $551.42\;{\mu}g/g$, 잎들깨1호 $301.59\;{\mu}g/g$, YCPL $187.00\;{\mu}g/g$, 그리고 밀양9호는 $145.75\;{\mu}g/g$이었으며, 그 외 3-hexen-1-ol, trans-2-hexenal, $\beta-caryophyllene$ 그리고 $\alpha-farnesene$등이 검출되었으나 이 화합물들의 함량은 $20\;{\mu}g/g$을 초과하지 않았다.

The present investigation was carried out to determine the qualitative difference between 4 varieties of perilla leaves cultivated Miryang area, particularly focusing on the amino acid composition and flavor compounds. Aspartic acid, glutamic acid, glycine, alanine, arginine, and threonine were the major amino acids, and tyrosine and cysteine and methionine containing sulfur were detected as little amount in protein of all perilla leaves. All perilla leaves contained about 34 kinds of free amino acids, and the major free amino acids were same as the protein bound amino acids. However, no significant difference among 4 varieties of perilla leaves was observed in the profiles of protein bound and free amino acids composition. Flavor compounds analyzed by GC-MSD following to extraction of flavor by SDE (Simultaneous Steam Distillation-Extraction) were detected as 51 kinds from 'Leafy perilla 1', 47 kinds from 'Yupsil perilla', 46 kinds from 'Miryang 9' and 'YCPL. The major volatile compound was perilla ketone, its concentration was $145.75\;{\mu}g/g$ in 'Miryang 9', $187.00\;{\mu}g/g$ in 'YCPL', $301.59\;{\mu}g/g$ in 'Leafy perilla l' and $551.42\;{\mu}g/g$ in 'Yupsil perilla', but the other flavor compounds, 3-hexen-l-ol, trans-2-hexenal, $\beta-caryophyllene,\;\alpha-farnesene$ and etc, were less than $20\;{\mu}g/g$.

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참고문헌

  1. Farrell, K. T. 1985. Spices, condiments, and seasonings. In 'Spices and culinary herbs' AVI Publishing, New York, 25
  2. Hong, Y. P., S. Y. Kim, and W. Y. Choi. 1986. Postharvest changes in quality and biochemical components of perilla leaves. J. of Korean Food Sci. Technol. 18, 255-258
  3. Hyun, K. W., K. C. Koo, J. H. Jang, J. G. Lee, M. R. Kim, and J. S. Lee. 2004. Quality Characteristics and flavor compounds of Geumsan perilla leaves cultivated in greenhouse and field. Korean J. of Food Preservation 11, 28-33
  4. Kang, H. S., S. Y. Lim, C. W. Nam, S. K. Yoon, and S. M. Byun. 1996. Studies on the volatile flavor components in perilla leaves. J. Life Sci. Dongkug Univ. 25, 509-528
  5. Kang, J. M., I. H. Cha, Y. G. Lee, and H. S. Rhu. 1997. Identification of volatile essential oil, and flavor characterization and antibacterial effect of fractions from Houttuynia cordata Thub. Ⅰ. Identification of volatile essential oil compounds from Houttuynia cordata Thunb. J. Korean Soc. Food Sci. Nutr. 26, 209-213
  6. Kang, J. M., I. H. Cha, Y. G. Lee, and H. S. Rhu. 1997. Identification of volatile essential oil, and flavor characterization and antibacterial effect of fractions from Houttuynia cordata Thub. Ⅱ. Flavor characterization and antibacterial effect of fraction from Houttuynia cordata Thunb by Prep-HPLC. J. Korean Soc. Food Sci. Nutr. 26, 214-221
  7. Kim K. H., M. W. Chang, K. Y. Park, S. H. Rhee, T. H. Rhew, and Y. Sunwoo. 1993. Antitumor activity of phytol identified from perilla leaf and its augmentative effect on cellular immune response. J. Korean Soc. Food Nutr. 22, 379-389
  8. Kim, K. S., S. N. Ryu, J. S. Song, J. K. Bang, and B. H. Lee. 1999. Comparision of analytical methods for volatile flavor compounds in leaf of Perilla frutescens. Korean J. Crop Sci. 44, 154-158
  9. Kim, Y. S., J. H. Lee, M. N. Kim, W. G. Lee, and J. O. Kim. 1994. Volatile flavor compounds from raw mugwort leaves and parched mugwort tea. J. Korean Soc. Food Nutr. 23, 261-267
  10. Koezuka, Y., G. Honda, and M. Tabata. 1986. Genetic control of the genetic control composition of volatile oils in Preilla frutescens. Phytochemistry 25, 859-863 https://doi.org/10.1016/0031-9422(86)80017-6
  11. Kwon, Y. J., Y. H. Kim, J. J. Kwag, K. S. Kim, and K. K. Yang. 1990. Volatile components of apricot (Prunus armanica var. ansu Max.) and Japanese apricot (Prunus Mume Sieb. et Zucc.). J. Korean Agric. Chem. Soc. 33, 319-324
  12. Lee, J. W., J. G. Lee, J. H. Do, and H. S. Sung. 1995. Volatile flavor components of Korean Auricularia polytricha (Mont.) Sacc. mushroom. Agric. Chem. Biotechnol. 38, 546-548
  13. Lee, K. I., S. H. Rhee, and K. Y. Park. 1999. Anticancer activity of phytol and eicosatrienoic acid identified from perilla leaves. J. Korean Soc. Food. Nutr. 28, 1107-1112
  14. Lee, K. I., S. K. Rhee, K. Y. Park, and J. O. Kim. 1992. Antimutagenic compounds identified from perilla leaf. J. Korean Soc. Food. Nutr. 21, 302-307
  15. Maneepun, S., B. S. Luh, and R. B. Rucker. 1974. Amino acid composition and biological quality of lima bean protein. J. of Food Science 39, 171-174 https://doi.org/10.1111/j.1365-2621.1974.tb01015.x
  16. Meredith, F. I. and W. O. Caster. 1984. Amino acid content in selected breakfast cereals. J. of Food Science 49, 1624-1625 https://doi.org/10.1111/j.1365-2621.1984.tb12863.x
  17. Nagy, J. C. 1966. Volatile oils and antibiosis of artemisia. ph. D. thesis, Colorado State Univ
  18. National Pural Living Science Institute, R.D.A. 2001. Food composition table(6th Revision). Sangrogsa, Korea, 96-97
  19. Ogawa, T., Y. Oka, and K. Sasaoka. 1987. Amino acid profiles of common cultivated mushrooms including the identification of N-(N-$\Upsilon$-L-glutamyl-3-sulfo-L-alanyl)glycine in Flammulina velutipes. J. of Food Science 52, 135-137 https://doi.org/10.1111/j.1365-2621.1987.tb13989.x
  20. Oka, Y., H. Tsuji, T. Ogawa, and K. Sasaoka. 1981. Quan titative determination of the free amino acids and their derivatives in the common edible mushroom, Agaricus bisporus. J. of Nutr. Sci. Vitaminol. 27, 253 https://doi.org/10.3177/jnsv.27.253
  21. Shin, K. K., C. B. Yang, and H. Park. 1992. Studies on lipid and fatty acid composition of Korean perilla leaves (Perilla frutescens var. japonica HARA). Korean J. Food Sci. Technol. 24, 610-615
  22. Sung, W. S. 1976. Studies on the element of domestic perilla. Korean J. Food Sci. Technol. 5, 69-74
  23. Tada, M., R. Matsumoto, H. Yamaguchi, and K. Chiba. 1996. Novel antioxidants isolated from Perilla frutescens Britton var. crisp (Thunb). Biosci. Biotech. Biochem. 60, 1093-1095 https://doi.org/10.1271/bbb.60.1093