산업용 단백분해효소로 처리한 분리대두단백과 혼합균주의 종류가 frozen soy yogurt의 품질특성에 미치는 영향

The quality characteristics of frozen soy yogurt prepared with soy protein isolate, industrial proteases and commercial mixed cultures

  • 이숙영 (중앙대학교 식품영양학과) ;
  • 박미정 (중앙대학교 식품영양학과)
  • Lee Sook-Young (Department of Food and Nutrition, Chung-Ang University) ;
  • Park Mi-Jung (Department of Food and Nutrition, Chung-Ang University)
  • 발행 : 2004.12.01

초록

In order to develop and commercialize high quality frozen soy yogurt, the effects of industrial proteases and commercial mixed cultures were examined on the functional properties and the sensory attributes of frozen soy yogurt. For quality improvement, soy protein isolates were primarily hydrolyzed by either Flavourzyme or Neurtrase, industrial Proteases, to reduce the beany flavor and increase the functional properties of the protein. The viable cell count of lactic acid bacteria was higher in the soy protein hydrolysates than whenuntreated. ABT-5 (L. acidophilus, Bifidobacterium lactis, and S. thermophilus) resulted in higher acid tolerance, bile acid tolerance and melt-down percent values than those with YC-X11 (Lactobacillus bulgaricus and Streptococci thermophilus). The overrun of frozen soy yogurt was improved by both Flavourzyme $(193.3\%)$ and Neurtrase $(156.7\%)$ treatments. With regard to thesensory characteristics, Flavourzyme improved the beany flavor, astringency taste, mouth feel and overall quality of frozen soy yogurts fermented with ABT-5. Further studies onproduct formulation will be needed to commercialize the frozen soy yogurt for the market.

키워드

참고문헌

  1. Tamine, AY, Mashall, VM and Robinson, RK : Microbiologieal and technological aspects of milk fermented by bifidobacteria. J. Dairy Res,, 62:151, 1995 https://doi.org/10.1017/S002202990003377X
  2. Back, YJ : 발효유산업의 연구개발 현황과 전망. Kor. J. Appl. Microbiol. Bioeng. 8(2):2026, 1995b
  3. Kwon, YS : Effects of Bifidobacteria and oligosaccharideson the quality attributes of frozen soy yogurts containing enzyme treated soy protein isolate. M. S. thesis, ChungAng Univ. Seoul, Korea, 1998
  4. Lee, JE and Lee, SY : Quality characteristics of frozen soy yogurt prepared with different proteolytic enzymes and starter culture. Korean J. Food Sci. Technol.,33(6):676, 2001
  5. Rasic, JL and Kurmarm, JA : Yoghurt. Technical dairy publising house, Copenhagen, Denmark, p.85, 1978
  6. Kim(Lee), SY, Park, SW and Rhee, KC : Textural properties of cheese analogs containing proteolytic enzyme modified soy protein isolate. J. Am. Oil Chem. Soc.,6:755, 1992
  7. Tuitemwong, P, Erickson, LE, Fung, DYC, Setser, CS and Pemg, SK : Sensory analysis of soy yogurt and frozen soy yogurt produced from rapid hydration hydrothermal cooked soy milk. J. Food Quality, 16:223, 1993 https://doi.org/10.1111/j.1745-4557.1993.tb00108.x
  8. loanna, S, Martinou, V and Gregory, KZ : Effect of some stabilizer on textural and sensory characteristics of yogurt ice cream from sheep milk. J. Food Sci., 55(3):703, 1990 https://doi.org/10.1111/j.1365-2621.1990.tb05211.x
  9. Jayne, EH and Joseph, PF : Viability of Lactobacilus acidophitus and Bifidobacterium bifidum in soft-serve frozen yogurt. Cult. Dairy Prod. J., August, p.4, 1991
  10. Berrada, N, Lemeland, JF, Laroche, PT and Piaia, M : Bifidobacterium from fermented milks, Survival during gastric transit. J. Dairy Sci., 74(2):409, 1991 https://doi.org/10.3168/jds.S0022-0302(91)78183-6
  11. Lee, CH, Kim, CS and Lee, SP : Studies on the enzymatic partial hydrolysis of soybean protein isolates. Korean J. of Food Sci. Technol. 16(2):228, 1984
  12. Heo, KC and Yoon, YH : Associative growth of Bifidobacterium sp. and Lactobacillus acidophilus in skim milk Korean J. Dairy Sci. 18(3):185, 1996
  13. Kim, HJ and Ko, YT : Study on preparation of yogurt from milk an soy protein. Korean J. of Food Sci. Technol. 22(6):700, 1990
  14. Hoch, MH, Henry, MS and Binder, MD : The effect of bile acids on intestinal microflora. Am. J. Clin. Nutri., 25:1418, 1972
  15. Gilliland, SE, Staley, TE and Bush, U : Importance of bile tolerance of Lactobacittus acidophilus used as adietary adjunct. J. Dairy Sci., 67:3045, 1984
  16. Shan, N and Jelen, P : Survival of lactic acid bacteria and their lactases under acidic conditions. J. Food Sci., 55(2):506, 1990 https://doi.org/10.1111/j.1365-2621.1990.tb06797.x
  17. Shin, YS, Sung, HJ, Kim, DH and Lee, KS : Survival rate of lactic acid bacteria and the change of $\beta$-galactosidase acticity of commercial yogurts under the acidic condition. Agricultural Chemistry and Biotechnology.37(3): 143, 1994b
  18. Hood, SK and Zottola, EA : Effect of low pH on the ability of Lactobacittus acidophiIus to survive and adhereto human intestinal cells. J. Food Sci., 53(5): 1514, 1988 https://doi.org/10.1111/j.1365-2621.1988.tb09312.x
  19. Shin, WS and Yoon, S : Effect of stabilizers on the texture of frozen yogurt. Korean J. Soc. Food Sci., 12:20, 1996
  20. Mitten, HL : Hard freezing yogurt mixes. Am. DairyRev., 39(6):23, 1977
  21. Rajor, RB and Gupta, SK : Soft-serve ice cream from soybean and butter milk I. Method of manufacture. Indian J. Dairy Sci., 35(4):454, 1982
  22. Mahdi, SR and Bradley, RL : Fat destabilization in frozen yogurt dessert containing low dextrose equivalent corn sweetner. J. Dairy Sci., 52(11): 1738, 1969 https://doi.org/10.3168/jds.S0022-0302(69)86836-0
  23. Lee, CH, Kim, CS and Lee, SP : Studies on the enzymatic partial hydrolysis of soybean protein isolates. Korean J. of Food Sci. Technol. 16(2)228, 1984