Conversion of Citron (Citrus junos) Peel Oil by Enterobacter agglomerans

  • PARK , YEON-JIN (Department of Food and Nutrition, Chosun University) ;
  • KIM, IN-CHEOL (Department of Food Engineering, Mokpo National University) ;
  • BAEK, HYUNG-HEE (Department of Food Engineering, Dankook University) ;
  • BANG, OK-KYUN (Department of Food and Medicine, Chosun University) ;
  • CHANG, HAE-CHOON (Department of Food and Nutrition, Chosun University, Department of Food and Medicine, Chosun University)
  • Published : 2004.12.01

Abstract

Citron peel oil was extracted from citron (Citrus funas) fruit by steam distillation, and was used as starting material for microbial conversion to synthesize attractive flavor compounds by using Enterobacter agglomerans 6L. E. agglomerans was isolated from citron peel and was able to metabolize the citron peel oil and grew well ($A_{600}:\;3.0$) on the citron peel oil as the sole carbon source. Multiple terpene metabolites were produced by E. agglomerans 6L on M9 salt media with citron oil vapor. The identified bioconversion products from the citron peel oil included trans-2-decenal, octanol, $\delta$­valerolactone, $\gamma$-valerolactone, cryptone, hydroxycitronellol, cuminol, and $\gamma$-dodecalactone.

Keywords

References

  1. Cadwallader, K. R., R. J. Braddock, and M. E. Parish. 1992. Isolation of $\alpha$-terpineol dehydratase from Pseudomonas gladioli. J. Food Sci. 57: 241-248
  2. Cadwallader, K. R., R. J. Braddock, M. E. Parish, and D. P. Higgings. 1989 Bioconversion of (+)-limonene by Pseudomonas gladioli. J. Food Sci. 54: 1241-1245
  3. Chang, H. C., D. A. Gage, and P. J. Oriel. 1995. Cloning and expression of a limonene degradation pathway from Bacillus stearothermophilus in Escherichia coli. J. Food Sci. 60: 551-553
  4. Chang, H. C. and P. Oriel. 1994. Bioproduction of perillyl alcohol and related monoterpenes by isolates of Bacillus stearothermophillus. J. Food Sci. 59: 660-662.+686
  5. Chung, J. and Y. Lee. 1998. Manufacture condition of oleoresin using citron peel. Korean J. Food Sci. Technol. 30: 139-145
  6. Feranoli, G. 1975. Handbook of Flavor Ingredients. 2nd Ed. CRC Press, Boca Raton, FL, U.S.A
  7. Hwang, Y. H. and H. S. Lee. 2002. Antibacterial activity of Pinus densiflora leaf-derived components toward human intestinal bacteria. J. Microbiol. Biotechnol. 12: 610-616
  8. Krasnobajew, V. 1984. In K. Lieslich (ed.). Biotechnology- Biotransformations. Weinheim 6a: 98-125
  9. Mo, E. K., H. J. Kang, C. T. Lee, B. J. Xu, J. H. Kim, Q. J. Wang, J. C. Kim, and C. K. Sung. 2003. Identification of phenylethyl alcohol and other volatile flavor compounds from yeast, Pichia farinose SKM-1, Pichia anomala SKMT, and Galactomyces geotrichum SJM-59. J. Microbiol. Biotechnol. 13: 800-808
  10. Park, Y. J., I. C. Kim, and H. C. Chang. 2003. Microbial conversion of (+)-limonene by an Enterobacter agglomerans isolate. J. Microbiol. Biotechnol. 13: 636-639
  11. Rama Devi, J. and P. K. Bhattacharyya. 1977. Microbiological transformations of terpenes: Part XXIV. Pathways of degradation of linalool, geraniol, nerol and limonene by Pseudomonas incognita. Indian J. Biochem. Biophy. 14: 359-363
  12. Ryu, K.-G., Y.-R. Chae, and O-Y. Kwon. 2003. Oxidation of dibenzothiophene catalyzed by surfactant-hemoprotein complexes in anhydrous nonpolar organic solvents. J. Microbiol. Biotechnol. 13: 647-650
  13. Sadtler Research Laboratories. 1986. The Sadtler Standard Gas Chromatography Retention Index Library. Sadtler, U.S.A
  14. Song, H. S., M. Sawamura, T. Ito, K. Kawashimo, and H. Ukeda. 2000. Quantitative determination and characteristic flavour of Citrus junos (yuzu) peel oil. Flavour Fragrance J. 15: 245-250
  15. van der Werf, M., P. M. Keijzer, and P. H. van der Schaft. 2000. Xanthobacter sp. C20 contains a novel bioconversion pathway for limonene. J. Biotechnol. 84: 133-143