KSBB Journal

  • 제19권5호
  • /
  • Pages.394-398
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  • 2004
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  • 1225-7117(pISSN)
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  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

질량 분석기를 이용한 트렌스아미나제 반응 분석을 위한 케토산 화학 수식법 개발

Derivatization of a-ketoacid for the Analysis of Aminotransferase Reaction Using Mass Spectrometry

  • 이창수 (충남대학교 공과대학 신소재 공학부) ;
  • 김은곤 (서울대학교 공과대학 응용화학부) ;
  • 김은미 (서울대학교 공과대학 응용화학부) ;
  • 김병기 (서울대학교 공과대학 응용화학부)
  • Lee Chang Soo (Department of Chemical Engineering, Chungnam National University) ;
  • Kim Yun Gon (School of Chemical Engineering and Institute of Molecular Biology and Genetics, Seoul National University) ;
  • Kim Eun Mi (School of Chemical Engineering and Institute of Molecular Biology and Genetics, Seoul National University) ;
  • Kim Byung Gee (School of Chemical Engineering and Institute of Molecular Biology and Genetics, Seoul National University)
  • 발행 : 2004.10.01
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초록

A derivatization method in mass spectrometry for small molecular analysis was developed to solve the problems of volatility of many analytes, difficult ionization of analytes, and undiscriminating isobaric analytes. This derivatization method, oximation of $\alpha$-ketoacid, in the transaminase reaction leads to change of mass difference between amine reactants and ketoacid products. In addition, regardless of the kinds of ketoacid, the linear relationship between the peak intensity and its concentration (from 1 mM to 10 mM) shows that quantitative analysis of the conversion of the reaction can be executed by the analysis of peak intensity of the corresponding oximated ketoacids. Furthermore, this method can be used for identifying transaminase as well as determining its substrate specificity

키워드

참고문헌

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