IMMUNE NETWORK
- 제4권4호
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- Pages.229-236
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- 2004
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- 1598-2629(pISSN)
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- 2092-6685(eISSN)
GD2 유사 항이디오타입 항체의 세포면역 유발 잠재성
Potentiality of Anti-idiotypic Antibodies Mimicking GD2 to Induce Cellular Immunity
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Park, Yoon-Sun
(Department of Microbiology, Kwandong University College of Medicine) ;
- Shin, Woon-Seob (Department of Microbiology, Kwandong University College of Medicine)
- 발행 : 2004.12.30
초록
Background: Disialoganglioside GD2 is a tumor-associated antigen that is overexpressed on tumor cells of neuroectodermal origin, such as melanoma, small cell lung carcinoma and neuroblastoma. Immunity against GD2 has anti-tumor activities, but GD2 is poorly immunogenic. Anti-idiotypic antibodies that mimic GD2 may induce more effective immune responses than GD2 antigen itself, because they are protein antigens and are known to be able to break immune tolerance. In our previous study, we produced anti-idiotypic antibodies mimicking GD2 (3A4 and 3H9), which induced humoral immunity. However, cellular immunity is essential to eradicate tumor cells in vivo as well as humoral immunity. In the present study, we investigated whether these anti-idiotypic antibodies 3A4 and 3H9 could induce cellular immunes responses. Methods: BALB/C mice were immunized with anti-idiotypic antibody 3A4 or 3H9, or normal mouse IgG as a negative control. Lymphoproliferative responses, cytokine production responses, and delayed-type hypersensitivity reactions were measured in mice immunized with the anti-idiotypic antibodies. Results: Both the anti-idiotypic antibody 3A4 and 3H9 induced GD2-specific lymphoproliferative responses and