Journal of the Korea Organic Resources Recycling Association (유기물자원화)
- Volume 12 Issue 1
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- Pages.67-74
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- 2004
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- 1225-6498(pISSN)
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- 2508-3015(eISSN)
Isolation and identification of a phenol-degrading bacterium from the sewage sludge
하수슬러지로부터 페놀분해세균의 분리 및 동정에 관한 연구
- Kim, Young-Jun (Division of Biotechnology, The Catholic University of Korea) ;
- Lee, Suk-Won (Division of Biotechnology, The Catholic University of Korea) ;
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Han, Gee-Bong
(Division of Biotechnology, The Catholic University of Korea)
- Received : 2004.02.09
- Accepted : 2004.03.15
- Published : 2004.03.30
Abstract
A bacterium which grow on phenol as an only carbon and energy source was isolated from the sewage sludge at Nangi municipal wastewater treatment plant in Seoul. This bacterium was found to be a Gram negative rod with high motility, and well grew on 0.05%, 0.1%, and 0.15% of phenol. No matching strain was found from the result of the BBL test. Phylogenetic analysis of the strain by comparison of the 16s-rDNA has revealed that this bacterium has 99% of similarity with Stenotrophomonas maltophilia strain of Xanthomonas group, which belongs t the Gamma (
난지도 하수처리장내 슬러지로부터 페놀을 유일한 탄소 및 에너지원으로 이용하는 세균을 분리하여 그 특성을 조사하고 동정하였다. 본 세균은 그람 음성 구균으로 운동성을 가지며 페놀의 농도가 0.05%, 0.10%, 0.15%인 배지에서 성장을 보였으며, BBL Test를 실시한 결과 유사성을 가진 세균이 나타나지 않았다. 세균의 계통분류학적 분석을 위하여 세균의 염색체로부터 16s rDNA를 클로닝한 후 DNA 염기서열을 파악하고 이를 비교 및 분석한 결과 본 세균은 Proteobacteria의 Gamma (
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