Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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Effects of Acupuncture and Electroacupuncture on the Doublecortin, PSA-NCAM and pCREB Expression in the Brain of Spontaneously Hypertensive Rats

침(鍼) 및 전침(電鍼)이 SHR 대뇌(大腦)에서 Doublecortin, PSA-NCAM, pCREB 양성 신경세포에 미치는 영향

  • Park, Jung-hwan (Department of Acupuncture & Moxibustion, Oriental Medical Hospital, Kyung Hee University) ;
  • Lee, Jae-dong (Department of Acupuncture & Moxibustion, Oriental Medical Hospital, Kyung Hee University) ;
  • Kim, Chang-hwan (Department of Acupuncture & Moxibustion, Oriental Medical Hospital, Kyung Hee University)
  • 박정환 (경희대학교 한의과대학 침구학교실) ;
  • 이재동 (경희대학교 한의과대학 침구학교실) ;
  • 김창환 (경희대학교 한의과대학 침구학교실)
  • Received : 2004.03.24
  • Accepted : 2004.05.15
  • Published : 2004.06.20
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Abstract

Background and Objective : The aim of this study was to investigate the effects of acupuncture and electroacupuncture on the DCX, PSA-NCAM, and pCREB expression in the brain of spontaneously hypertensive rats(SHR). Materials and Methods : SHR were divided into five groups: control group, acupuncture group, 2Hz electroacupuncture(EA) group and 100Hz EA group. We evaluated the changes of the DCX, PSA-NCAM, and pCREB positive cells using immunohistochemical method. In the olfactory bulb, we investigate the optical densities of the immunoactive cells. In the dentate gyrus and the piriform cortex, we count the immunoactive cells under the $100{\times}$ visual field optical microscope. Results : 1. The optical densities of DCX-positive cells in the subependymal zone were significantly decreased in all groups, compared to the control group. 2. The counts of DCX-positive cells in the dentate gyrus were significantly increased in all groups, compared to the control group. The counts of DCX-positive cells in the piriform cortex were significantly increased in the acupuncture and 100Hz EA group, compared to the control group. 3. The optical densities of PSA-NCAM-positive cells in the subependymal zone were significantly decreased in the acupuncture and 2Hz EA group, compared to the control group. 4. The counts of PSA-NCAM-positive cells in the dentate gyrus and the piriform cortex were significantly increased in all group, compared to the control group. 5. The counts of pCREB-positive cells in the dentate gyrus were significantly increased in all groups, compared to the control group. The counts of pCREB-positive cells in the piriform cortex were significantly increased in the acupuncture and 100Hz EA group, compared to the control group. Conclusion : We conclude that acupuncture and EA may affect neuronal cell proliferation, differentiation and plasticity in the brain.

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