Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

Protective effect of Juglans sinensis Dode extract (JS) on oxidant-induced apoptosis in renal epithelial cells

신세뇨관(腎細尿管) 상피세포(上皮細胞)에서 산화(酸化)로 유발(誘發)된 apoptosis에 대한 호도약침액(胡桃藥鍼液)의 방어효과(防禦效果)

  • Park, In-bum (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Ahn, Chang-beohm (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Jang, Kyung-jeon (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Song, Choon-ho (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Yoon, Hyoun-min (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Kim, Cheol-hong (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University)
  • 박인범 (동의대학교 한의과대학 침구경혈학교실) ;
  • 안창범 (동의대학교 한의과대학 침구경혈학교실) ;
  • 장경전 (동의대학교 한의과대학 침구경혈학교실) ;
  • 송춘호 (동의대학교 한의과대학 침구경혈학교실) ;
  • 윤현민 (동의대학교 한의과대학 침구경혈학교실) ;
  • 김철홍 (동의대학교 한의과대학 침구경혈학교실)
  • Received : 2004.03.11
  • Accepted : 2004.05.15
  • Published : 2004.06.20
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Abstract

Objective: This study was undertaken to evaluate the role of lipid peroxidation in oxidant-induced apoptosis and effect of JS on the apoptosis in opossum kidney (OK) cells, an established renal proximal tubular cells. Methods : Exposure of cells to 0.1mM tBHP for 2hr did not induce apoptosis, but subsequent incubation in normal culture medium for 18hr after tBHP treatment induced apoptotic cell death which is dependent of tBHP concentration. Results : JS decreased tBHP-induced apoptotic cell death in a dose-dependent fashion and at concentrations higher than 0.01 mg/ml completely prevented the apoptosis. tBHP-induced apoptosis was prevented by the lipid soluble antioxidant N,N'-diphenyl-p-phenylenediamine (DPPD) and water-soluble antioxidant Trolox. tBHP increased lipid peroxidation, which was inhibited by JS and DPPD. tBHP-induced DNA damage was prevented by JS and DPPD. Conclusion : These results indicate that tBHP induces apoptosis through a lipid peroxidation-dependent mechanism and JS exerts the protective effect against the apoptosis by preventing peroxidation of membrane lipids.

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