Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Plant Regeneration from Seed-derived Callus in Kentucky Bluegrass(Poa pratensis L.)

켄터키 블루그래스의 종자유래의 캘러스로부터 식물체 재분화

  • Yoon Ho-Sung (Institute of Agricultural Science and Technology, Kyungpook National University) ;
  • Lee Myunghee (Yeongnam Agricultural Research Institute) ;
  • Bae Eunkyung (Department of Animal Science, Kyungpook National University) ;
  • Lee Hyoshin (Biotechnology Division, Korea Forest Research Institute) ;
  • Jo Jinki (Department of Animal Science, Kyungpook National University)
  • 윤호성 (경북대학교 농업과학기술연구소) ;
  • 이명희 (작물과학원 영남농업연구소) ;
  • 배은경 (경북대학교 농업생명과학대학 동물공학과) ;
  • 이효신 (임업연구원 생물공학과) ;
  • 조진기 (경북대학교 농업생명과학대학 동물공학과)
  • Published : 2004.09.01
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Abstract

Plant regeneration from seed-derived callus of Kentucky bluegrass(Poa pratensis L. cv. Kenblue) was investigated. Callus induced on the medium supplemented with 2 mg/L 2,4-D and 0.2 mg/L BAP showed highest frequency of plant regeneration on the regeneration medium supplemented with 1 mg/L NAA and 5 mg/L kinetin. Callus induced in the dark condition showed higher regenerability than that induced in the dim light. MS medium was better than N6 and B5 medium in enhancing plant regeneration. Maltose was superior to sucrose in plant regeneration as carbon source in the medium.

켄터키 블루그래스의 종자배양에서 캘러스 형성 및 식물체 재분화 체계를 확립하기 위하여 배지조성 및 배양조건 등에 따른 배양효율의 차이를 'Kenblue'를 공시품종으로 사용하여 수행한 실험의 결과를 요약하면 다음과 같다. 성숙종자로부터 캘러스 유도를 위한 생장조절제로는 2 mg/L 2,4-D와 0.2 mg/L BAP 조합이 가장 효과적이었으며, 유도된 캘러스로부터 식물체 재분화를 위해서는 1 mg/L NAA와 5mg/L kinetin 조합이 가장 효과적이었다. 캘러스 유도시 암상태에서 배양하는 것이 약한 광 상태에서 배양하는 것보다 효과적이었으며, 캘러스 유도와 식물체 재분화에 있어 기본배지로 N6 배지나 B5 배지보다 MS 배지를 사용하는 것이 식물체 재분화에 효과적이었다. 배지 내에 첨가되는 탄소원으로는 maltose가 sucrose 보다 효과적이었다.

Keywords

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