Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Rockfish (Sebastes schlegeli) Vitellogenin: Purification, Characterization and Development of Sandwich ELISA System

  • Published : 2004.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Vitellogenin (VTG) was purified from serum of $estradiol-l7{\beta}-treated rockfish$(Sebastes schlegeli) by precipitation with $EDTA-Mg^{2+}$ and ammonium sulfate and two step chromatography (anion exchange chromatography and gel permeation chromatography) was performed on FPLC system. Rockfish VTG (rfVTG) was characterized and its properties were determined. The monomers have apparent, molecular mass of about 188 kDa as indicated by SDS-PAGE. Amino acid composition analysis of rfVTG was similar to VTG from other oviparous teleosts. Cysteine and lysine were present at relatively high level. Leucine was present at relatively lower level than in other species. The N-terminal amino acid sequence was evaluated to identify rfVTG. Western blot analysis using an antibody against the purified VTG showed that the antibody reacted with both plasma of $estradiol-l7{\beta}-treated rockfish$ treated male and purified VTG, whereas there was no reaction with male serum of the control. An ELISA was developed using monoclonal and polyclonal antibodies against rfVTG. The assay range was 3.2 ng/mL and 1,000 ng/mL and the value of the intra and inter assay variations were within $9.7{\%}$ and $11.2{\%}$, respectively. Recovery rate was $96.8{\%}$. The sandwich ELISA could be useful for the detection of VTG and could be good for screening of estrogenic compounds.

Keywords

References

  1. Andrew, E.G., M.G. John, T.B. James and H.E. Barbara. 1992. Monoclonal antibody based immunoassay of vitellof,enin in the blood of male channel catfish (lctalurus punctatus). Comp, Biochem. Physiol., 101B, 441-446
  2. Babin, P,J. 1992, Binding of thyroxine and 3,5,3'-triodothyroxine to trout plasma lipoproteins. Am. J. Physio., 262E, 712-720
  3. Boehlert, Y. 1991. Rockfishes of the Genus Sebastes: Their reprodlction and early life history. Klower Academic Publisters. Developments in environmental biology of fishes II. Boston, London, pp. 49~56
  4. Bon, E., L. Barbe and J.L Rodriguez. 1997. Plasma vitellogenin levels during the annual reproductive cycle of the female rainbow trout (Oncorhynchus mykiss ): establishment and validation of an ELISA. Compo Biochem. Physiol., 117B, 75-84
  5. Danie, P.L. T.R. William, D.M. James and L.J. Lyndal. 1998. An enzyme-linked immunosorbent assay (ELISA) for measuring vitellogenin in English sole (Pleuronectes vetulus): Development, validation and cross-reactivity with other pleuronectids. Comp Biochem. Physiol., 116B, 425-436
  6. De Vlaming, L., H.S. Wiley, G. Delahunty and R.A. Wallace. 1980. Goldfish (Carassius auratus) VTG: inducti'm, isolation, properties and relationship to yolk proteirs. Compo Biochem. Physiol., 67B, 613-623
  7. De Vlaming, L., D. Baltz, S. Aderson, R. Fitzgerald, G. Delahunty and M. Barkley. 1983. Aspects of embryo nutrition and excretion among viviparous embiotocid teleost Potential endocrine involvements. Compo Biochem. Physiol. 76A, 189-198
  8. Ding, J.L., P.L. Hee and T.J. Lam. 1989. Tow forms of vitello senin in the plasma and gonads of male Oreocnromis aureus. Comp Biochem. Physiol, 93B, 363-370
  9. Fenske, M., R. van Aerie, S. Brack, C.R. Tyler and H. Segner. 2001. Development and validation of a homologous zebrafish (Danio rerio Hamilton Buchanan) vitellogenin enzyme-linked immunosorbent assay (ELISA) and its application for studies on estrogenic chemicals. Compo Biochem. Physiol., 129, 217-232
  10. Folmar, L.C., N.D. Denslow, R.A. Wallace, G. LaFlueur, T.S. Gross, S. Bonomelli and C.V. Sullivan. 1995. A hig'lly conserved N-terminal sequence for teleost VTG with potential value to the biochemistry, molecJlar biology, and pathology of vitellogenesis. J. Fish BioI., 46, 255-263 https://doi.org/10.1111/j.1095-8649.1995.tb05966.x
  11. Finn, R.N., L. Ronnestad and H,J. Fyhn. 1995. Respiration, nitrogen and energy metabolism of developing yolksac larvae of Atlantic halibut (Hippglossus hippoglossu.: L.). Compo Biochem. Physiol., 111A, 647-671
  12. Fyhn, H.J. and B. Serigstad. 1987. Free amino acids as energy substrate in developing eggs and larvae of the cod Gadus morhua. Mar. BioI. 96, 335-341 https://doi.org/10.1007/BF00412514
  13. Hara, A., K. Yamauchi and H. Hirai. 1980. Studies on female-specific serum protein (VTG) and egg yolk protein in Japanese eel (Anguilla japonica). Comp. Biochem. Physiol. 65B, 315-320
  14. Harlow, E. and D. Lane, 1988. A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
  15. Mark, H., M. Wiesmann, B. Allner and H. Sauerwein. 2003. Vitellogenin in carp (Cyprinus carpio) and perch (Perca fluviatilis): Purification, characterization and development of an ELISA for the detection of estrogenic effects. Sci. Total Environ., 20, 93-103
  16. Johnsen, H.K., H. Tveiten, N.P. Villassen and A.M. Arnesen. 1999. Arctic charr (Salvelinus alinus) VTG: Development and validation of an enzyme-linked immunosorbent assay. Compo Biochem. Physiol., 124B, 355-362
  17. Kanungo, J., T.R. Petrino and P.A. Wallace. 1990. Oogenesis in Fundulus heteroclitus. VI. Establishment and verification of condition for vitellogenin incorporation by oocytes in vitro. J. Exp. Zool., 25, 313-321
  18. Kishida, M. and J.L. Specker. 1993. VTG in tilapia (Oreochromis mossambicus): Induction of two forms by estradiol, quantification in plasma and characterization in oocyte extact. Fish Physiol. Biochem., 12, 171-82 https://doi.org/10.1007/BF00004365
  19. Korsgaard, B. and I.M. Petersen. 1979. Vitellogenin, lipid and carbohydrate metabolism during vitellogenesis and pregnancy in the blenny (Zoarces viviparous L.). Comp. Biochem. Physiol., 63B, 245-251
  20. Korsgaard, B. and K.L. Petersen. 1998. Vitellogenin in Zoarces viviparous: Purification quantification by ELISA and induction by estradiol 17$\beta$ and 4-non-ylphenol. Comp. Biochem. Physiol., 120C, 159-166
  21. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 https://doi.org/10.1038/227680a0
  22. Lee, K.B.H., E.H. Lim, T.J. Lam and J.L. Ding. 1992. Vitellogenin diversity in the perciformes. J. Exp. Zool., 264, 100-106 https://doi.org/10.1002/jez.1402640115
  23. Lomax, D.P., W.T. Roubal, J.D. Morre and L.L. Johnson. 1998. An enzyme-linked immunosorbent assay (ELISA) for measuring vitellogenin in English sole (Pleruonectes vetulus): Development, validation and cross-reactivity with other pleuronectids. Compo Biochern. Physiol., 121, 425-436 https://doi.org/10.1016/S0305-0491(98)10125-6
  24. Mananose, E., S. Zanuy, F. Le Menn, M. Carrillo and J. Nunez. 1994. Sea bass (Dicentrarchus labrax L.) vitellogenin. I. Induction, purification and partial characterization. Comp. Biochem. Physiol, 107B, 205-216
  25. Matsubara, T., N. Ohkubo, T. Andoh, C.V. Sullivan and A. Hara. 1999. Two forms of vitellogenin, yielding two distinct lipovitellins, play different roles during oocyte maturation and early development of barfin flounder, Verasper moseri, a marine teleost that spawns pelagic eggs. Dev. Biol., 213, 18-32 https://doi.org/10.1006/dbio.1999.9365
  26. Moscoconi, G.O., R.M. Camevali, A.M. Nabissi and M. Polzonetti. 1998. Gilthead seabream (Sparus aurata) vitellogenin: Purification partial characterization and validation of on enzyme-linked immunosorbent assay (ELISA). Gen. Comp. Endocrinol., 110, 252-261 https://doi.org/10.1006/gcen.1998.7075
  27. Nish, K., M. Chikae, Y. Hatano, Y. Mizukami, H.M. Yamashita and R. Sakakibara. 2002. Development and application of monoclonal antibody-based sandwich for quantification of Japanese medaka (Oryzias latipes) vitelIogenin. Compo Biochem, Physiol., 132, 161-169 https://doi.org/10.1016/S1095-6433(01)00543-8
  28. Ng, T.B. and D.R. Idler. 1983. Yolk formation and differentiation in teleost fishes. In Fish. Physiol. Academic. Press. New Yolk, 4, 373-404
  29. Okumura, H., T. Todo, S. Adachi and K. Yamauahi. 2002. Changes in hepatic VTG mRNA levels during oocyte development in the Japanese eel, Anguilla japonica. Gen. Compo Endocrinol., 125, 9-16 https://doi.org/10.1006/gcen.2001.7716
  30. Parks, L.G., A.O. Cheek, N.D. Denslow, S.A. HeppelI, J.A. McLachlan, G.A LeBlanc and C.V. SulIivan. 1999. Fathead minnow (Pimephales promelas) VTG: Purification, characterization and quantitative immunoassay for the detection of estrogenic compounds. Compo Biochem. Physiol. 123C, 113-125
  31. Rodriguez, J.L., M. Kah, O. Geffard and F. Le Menn. 1989. Enzyme-linked immuno-sorbent assay (ELISA) for sole (Solea vulgaris) VitelIogenin. Comp. Biochem. Physiol., 92, 741-746 https://doi.org/10.1016/0305-0491(89)90260-5
  32. R$\phi$nnestad, I. and H.J. Fyhn. 1993. Metabolic aspect of free amino acids in developing marine fish eggs and larvae. Rev. Fish. Sci., II, 239-259
  33. Rnnestad, I., E.P. Groot and H.J. Fyhn. 1993. Compartmental disruption of free amino acids and protein in developing yolk-sac larvae of Atlantic halibut (Hippglossus hippoglossus). Mar. Biol., 116, 349-354 https://doi.org/10.1007/BF00350051
  34. Selman, K. and R.A. Wallace. 1983. Oogenesis in Fundulus heteroclitus, III. VitelIogenesis. J. Exp. Zool. 226, 441-457 https://doi.org/10.1002/jez.1402260315
  35. Selman, K. and R.A.Wallace. 1989. Cellular aspects of oocyte growth in teleosts. Zool. Sci. 6, 211-31
  36. Sherry, J, M. Fielden, P. Hodson, B. Bumison and K. Solomon. 1999. An ELISA for brown trout (Salmo trutta) VTG and its use in bioassays for environmental estrogens. Sci. Total. Environ., 225, 13-31 https://doi.org/10.1016/S0048-9697(99)80014-X
  37. So, Y.P., D.R. Idler and S.J. Hwang. 1985. Plasma vitellogenin the landlocked Atlantic salmon, Salmo salar: Isolation, homologous radioimmunoassay and immunological crossreactivity with vitellogenin from other teleosts. Comp. Biochem. Physiol., 81B, 63-71
  38. Sumpter, J.P. and S. Jobling. 1996. Vitellogenesis as a biomarker for estrogenic contamination of the aquatic environment. Environ. Health Perspect., 103, 173-178
  39. Takemura, A., A. Hara and K. Takano. 1991. Immunochemical identification and partial characterization of female-specific serum proteins in white-edged rockfish, Sebastes taczanowskii. Environ. Biol. Fish., 30, 49-56 https://doi.org/10.1007/BF02296876
  40. Tao, Y., A. Hara, R. Hodson, L.C. Woods and C.V. Sullivan. 1993. Purification, characterization and immunoassay of striped bass (Morone saxatilis) VTG. Fish Physiol. Biochem., 12, 31-46 https://doi.org/10.1007/BF00004320
  41. Tyler, C.R. and Sumpter. 1990. The purification and partial characterization of carp Cyprinus carpio, VTG. Fish Physiol. Biochem., 8, 111-120 https://doi.org/10.1007/BF00004438
  42. Utarabhand, P. and P. Bunlipatanon. 1996. Plasma VTG of grouper (Epinephelus malabaricus): Isolation end properties. Compo Biochem. Physiol., 115C, 101- 10
  43. Wallace, R.A. 1985. Vitellogenesis and oocyte growth in non-mammalian vertebrates. In: Developmental Biology, Pergamon Press, Elmsford, New York, pp. 1127-1177
  44. Wallace, R.A. and K. Selman. 1985, Major protein changes during vitellogenesis and maturation of Fundulus. Dev. Biol. 110, 492-498 https://doi.org/10.1016/0012-1606(85)90106-X
  45. Wiegan, M.D. 1982. VitelIogenesis in fishes. In Reproductive Physiology of Fish Pudoc Wageningen, The Nerherlands, pp. 136-146
  46. Zuxu, Y. and W.C. Laurence. 1996. A biochemical characterization of vitellogenins isolated from the marine fish ocean pout (Macrozoarces americanus L.), lumpfish (Cyclopterus lumpus) and Atlantic cod (Gadus morhua). Compo Biochem. Physiol., 113B, 247-253

Cited by

  1. Purification, Partial Characterization, and Immunoassay of Vitellogenin from Marbled Sole (Limanda yokohamae) vol.8, pp.4, 2004, https://doi.org/10.5657/fas.2005.8.4.213