Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

A study on the biodegradable novel chitosan nanofiber membrane as a possible tool for guided bone regeneration

키토산 나노 차폐막의 골조직 재생유도 능력에 관한 조직학적 연구

  • Shin, Seung-Yun (Department of Periodontology, College of Dentistry, Seoul National University, Intellectual Biointerface Engineering Center, KOSEF, NIBEC Inc.) ;
  • Park, Ho-Nam (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Kim, Kyoung-Hwa (Department of Periodontology, College of Dentistry, Seoul National University, Intellectual Biointerface Engineering Center, KOSEF, NIBEC Inc.) ;
  • Lee, Seung-Jin (Department of Industrial Pharmacy, College of Pharmacy, Ewha Womans University, Intellectual Biointerface Engineering Center, KOSEF, NIBEC Inc.) ;
  • Park, Yoon-Jeong (Craniomaxillofacial Reconstructive Science Major, College of Dentistry, Seoul National University, Intellectual Biointerface Engineering Center, KOSEF, NIBEC Inc.) ;
  • Ku, Young (Department of Periodontology, College of Dentistry, Seoul National University, Intellectual Biointerface Engineering Center, KOSEF, NIBEC Inc.) ;
  • Rhyu, In-Chul (Department of Periodontology, College of Dentistry, Seoul National University, NIBEC Inc.) ;
  • Han, Soo-Boo (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Chung, Chong-Pyoung (Department of Periodontology, College of Dentistry, Seoul National University, Intellectual Biointerface Engineering Center, KOSEF, NIBEC Inc.)
  • 신승윤 (서울대학교 치과대학 치주과학교실, 지능형 생체계면공학연구센터, KOSEF, (주)나이벡) ;
  • 박호남 (서울대학교 치과대학 치주과학교실) ;
  • 김경화 (서울대학교 치과대학 치주과학교실, 지능형 생체계면공학연구센터, KOSEF, (주)나이벡) ;
  • 이승진 (이화여자대학교 약학대학 물리약학교실, 지능형 생체계면공학연구센터, KOSEF, (주)나이벡) ;
  • 박윤정 (서울대학교 치과대학 두개악안면재건과학교실, 지능형 생체계면공학연구센터, KOSEF, (주)나이벡) ;
  • 구영 (서울대학교 치과대학 치주과학교실, 지능형 생체계면공학연구센터, KOSEF, (주)나이벡) ;
  • 류인철 (서울대학교 치과대학 치주과학교실, (주)나이벡) ;
  • 한수부 (서울대학교 치과대학 치주과학교실) ;
  • 정종평 (서울대학교 치과대학 치주과학교실, 지능형 생체계면공학연구센터, KOSEF, (주)나이벡)
  • Published : 2004.09.30
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Abstract

Chitosan has been widely researched as bone substitution materials and membranes in orthopedic/periodontal applications. Chitosan nanofiber membrane was fabricated by chitosan nanofiber using electrospinning technique. The structure of the membrane is nonwoven, three-dimensional, porous, and nanoscale fiber-based matrix. The aim of this study was to evaluate the biocompatibility of chitosan nanofiber membrane and to evaluate its capacity of bone regeneration in rabbit calvarial defect. Ten mm diameter round cranial defects were made and covered by 2 kinds of membranes (Gore-Tex membrane, chitosan nanofiber membrane) in rabbits. Animals were sacrificed at 4 weeks after surgery. Decalcified specimens were prepared and observed by microscope. Chitosan nanofiber membrane maintained its shape and space at 4 weeks. No inflammatory cells were seen on the surface of the membrane. In calvarial defects, new bone bridges were formed at all defect areas and fused to original old bone. No distortion and resorption was observed in the grafted chitosan nanofiber membrane. However bone bridge formation and new bone formation at the center of the defect could not be seen in Gore-Tex membranes. It is concluded that the novel membrane made of chitosan nanofiber by electrospinning technique may be used as a possible tool for guided bone regeneration.

Keywords

References

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