Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Effect of mouthrinse containing Lipomyces starkeyi KSM 22 glucanhydrolase on plaque formation during a 4-day period

Lipomyces starkeyi KSM 22 glucanhydrolase의 추가가 구강세정액의 치태 억제 효과에 미치는 영향

  • Seo, Eun-Ju (Dept. of Periodontology, College of Dentistry and Dental Science Research Institute, Chonnam National University) ;
  • Chung, Hyun-Ju (Dept. of Periodontology, College of Dentistry and Dental Science Research Institute, Chonnam National University) ;
  • Kim, Ok-Su (Dept. of Periodontology, College of Dentistry and Dental Science Research Institute, Chonnam National University) ;
  • Kim, Young-Jun (Dept. of Periodontology, College of Dentistry and Dental Science Research Institute, Chonnam National University) ;
  • Kim, Sang-Heuk (Lifeenza Co. Ltd)
  • 서은주 (전남대학교 치과대학 치주과학교실 및 치의학연구소) ;
  • 정현주 (전남대학교 치과대학 치주과학교실 및 치의학연구소) ;
  • 김옥수 (전남대학교 치과대학 치주과학교실 및 치의학연구소) ;
  • 김영준 (전남대학교 치과대학 치주과학교실 및 치의학연구소) ;
  • 김상혁 (주식회사 라이프엔자)
  • Published : 2004.03.30
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Abstract

A novel glucanhydrolase from Lipomyces starkeyi KSM 22 has been suggested as a promising anti-plaque agent because it has been shown to have additional amylase activity and mutanase activity besides dextranase activity and to strongly bind to hydroxyapatite. Mouthrinsing with Lipomyces starkeyi KSM 22 glucanhydrolase solution was comparable to 0.12% chlorhexidine mouthwash in inhibition of plaque accumulation and gingival inflammation and local side effects were less frequent and less intense in human experimental gingivitis. In this study, Lipomyces starkeyi KSM 22 glucanhydrolase mouthrinses (1 and 2 unit/ml) were compared with a control mouthrinse (commercial 0.01% benzethonium chloride mouthrinse, $Caregargle^{(R)}$, Hanmi Pharmaceuticals) in the ability to inhibit plaque formation. A 3-replicate clinical trial using 4-day plaque regrowth model was used. Fifteen volunteers were rendered plaque-free on the 1st day of each study period, ceased toothcleansing, and rinsed 2X daily with allocated mouthrinse thereafter. On day 5, plaque accumulation was scored and the washout periods was 9 days for the next trial. Lipomyces starkeyi KSM22 glucanhydrolase(1 unit and 2 unit)- containing mouthrinse resulted in Significantly lower plaque formation in plaque area and thickness, compared to the control mouthrinse. There was no significant difference in plaque inhibition between enzyme-mouthrinses at 2 different concentrations used. This glucanhydrolase- containing mouthwash resulted in significantly lower plaque area severity index score and tended to have lower plaque thickness severity index score than those of control mouthrinse. But there was no significant difference according to the enzyme concentration. From these results, Lipomyces starkeyi KSM 22 glucanhydrolase-containing benzethonium chloride mouthrinse has greater anti-plaque effect than the commercial mouthrinse alone. Therefore this glucanhydrolase preparation is a promising agent for new mouthwash formulation in the near future.

Keywords

References

  1. Lobene RR, Clinical studies of plaque control agents: an overview. J Dent Res 1979;58:2381-2388
  2. Johnson RH, Rozanis J. A review of chemotherapeutic plaque control. Oral Surg Oral Med Oral Pathol 1979;47:136-141 https://doi.org/10.1016/0030-4220(79)90168-3
  3. Hull PS. Chemical inhibition of plaque. J Clin Periodontol 1980;7:431-442 https://doi.org/10.1111/j.1600-051X.1980.tb02150.x
  4. Segreto VA, Collins EM, Beiswanger BB, De la Rosa MR, Issacs RL, Lang NP, Mallatt ME, Meckel AH, A Comparison of mouthwashes containing two concentrations of chlorhexidine. J Periodont Res 1986;21(Suppl 16):26-32
  5. 류인철. 수종의 상용 세치제들의 항균 및 항염효과 비교 연구. 대한치주과학회지 1996;26:557-566
  6. Banting D, Bosma M, Bollmer B. Clinical effectiveness of 0.12% chlorhexidine mouth rinse over two years. J Dent Res 1989;68:1716-1718
  7. Gjermo P. Chlorhexidine and related substances. J Dent Res 1981;68:1602-1608
  8. Walker GJ, Pulkownik A, Morrey-Jones JG. Metabolism of the polysacchrides of human dental plaque: release of dextranse in batch cultures of Streptococcus mutans. J Gen Microbiol 1981;127:201-208
  9. Hamada S, Mizuno J, Murayama Y, Ooshima Y, Masuda N. Effect of dextranase on the extracellular polysaccharide synthesis of Streptococcus mutans: chemical and scanning electron microscopy studies. Infect Immun 1975;12:1415-1425
  10. Wenham DG, Davis RM, Cole JA. Insoluble glucan synthesis by mutansucrase as a determinant of the cariogenicity of Streptococcus mutans. J Gen Microbiol 1981;127:407-415
  11. Goldstein-Lifschitz B, Bauer S. Comparison of dextranases of their possible use in eliminating dental plaque. J Dent Res 1976;55:886-892 https://doi.org/10.1177/00220345760550052901
  12. Schachtele CF, Staat RH, Harlander SK. Dextranase from oral bacteria: inhibition of water-insoluble glucan production and adherence to smooth surfaces by Streptococcus mutans. Infect Immun 1975;12:309-317
  13. Caldwell RC, Sandham HJ, Mann WV Jr, Finn SB, Formicola AJ. The effect of a dextranase mouth-wash on dental plaque in young adults and children. J Am Dent Assoc 1971;82:124-131 https://doi.org/10.14219/jada.archive.1971.0023
  14. Kim D, Ryu SJ, Hoe SJ, Kim DW, Kim HS. Characterization of a novel carbohydrase from Lipomyces starkeyi KSM 22 for dental application. J Microbiol Biotechnol 1999;9:260-264
  15. Ryu SJ, Kim D, Ryu HJ. Purification and partial characterization of a novel glucanhydrolase from Lipomyces starkeyi KSM 22 and its use for inhibition of insoluble glucan formation. Biosci Biotechnol Biochem 2000;64:223-228 https://doi.org/10.1271/bbb.64.223
  16. 손은주, 김영준, 김도만, 정현주. 실험적 치은염에서 dextranase 함유 구강 세정액의 양치효과. 대한치주과학회지 2001;31:401-420
  17. 송우성, 손은주, 김도만, 정현주. Dextranase 함유 구강 세정액의 치태 억제 및 치은염 예방 효과에 관한 임상적 연구. 대한치주과학회지 2001;31:371-388
  18. Addy M, Willis L, Moran J. Effect of toothpaste rinses compared with chlorhexidine on plaque formation during a 4-day period. J Clin Periodontol 1983;10:89-99 https://doi.org/10.1111/j.1600-051X.1983.tb01270.x
  19. Turesky S, Gilmore ND, Glickman I. Reduced plaque formation by the chloromethyl analogue of victamine C. J Periodontol 1970;41:41-43 https://doi.org/10.1902/jop.1970.41.41.41
  20. Ramfjord SP. Indices for prevalence and incidence of periodontal disease. J Periodontol 1959;30:51
  21. Newman MG, Takei HH, Carranza FA. Carranza's clinical periodontology, 9th ed. W. B. Saunders, 2002:84
  22. Loe H, Silness J. Periodontal disease in pregnancy. 1. Prevalence and severity. Acta Odontol Scand 1963;21:533-551 https://doi.org/10.3109/00016356309011240
  23. Allen DR, Davies R, Bradshaw B. Efficacy of a mouthrinse containing 0.05% cetylpyridinium chloride for the control of plaque and gingivitis: a 6-month clinical study in adults. Compend Contin Educ Dent 1998;19(2 Suppl):20-26
  24. Binney A, Addy M, McKeown S, Everatt L. The effect of a commercially available triclosan-containing toothpaste compared to a sodium-fluoride- containing toothpaste and a chlorhexidine rinse on 4-day plaque regrowth. J Clin Periodontol 1995;22:830-834 https://doi.org/10.1111/j.1600-051X.1995.tb01780.x
  25. Rosin M, Welk A, Bernhardt O, Ruhnau M, Pitten FA, Kocher T, Kramer A. Effect of a polyhexamethylene biguanide mouthrinse on bacterial counts and plaque. J Clin Periodontol 2001;28:1121-1126 https://doi.org/10.1034/j.1600-051X.2001.281206.x
  26. Lobene RR. 2. A clinical study of the effect of dextranase on human dental plaque. J Am Dent Assoc 1971;82:132-135 https://doi.org/10.14219/jada.archive.1971.0014
  27. Jeffcoat M. What is clinical significance? J Clin Periodontol 2002;29(Suppl 2):30-32
  28. Killoy WJ. The clinical significance of local chemotherapies. J Clin Periodontol 2002;29(Suppl 2):22-29
  29. 윤현정, 정현주, 김옥수, 김도만. Lipomyces starkeyi KSM 22 Glucanhydrolase 용액의 치은 섬유아세포에 대한 영향. 대한치주과학회지 2002;32:665-683