한국토양비료학회지 (Korean Journal of Soil Science and Fertilizer)

  • 제37권1호
  • /
  • Pages.31-35
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  • 2004
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  • 0367-6315(pISSN)
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  • 2288-2162(eISSN)
한국토양비료학회 (Korean Society of Soil Science and Fertilizer)
권호 표지

자외선에 의한 벼 엽 세포 소기관의 형태적 변화

Morphological Alteration of Cell Organelles Affected by UV-B Radiation in Rice Leaf Tissues

  • Sung, Jwa-Kyung (Department of Agronomy, Chungbuk National University) ;
  • Song, Beom-Heon (Department of Agronomy, Chungbuk National University) ;
  • Kim, Hong-Sig (Department of Agronomy, Chungbuk National University) ;
  • Lee, Chul-Won (Department of Agronomy, Chungbuk National University) ;
  • Kim, Tae-Wan (Department of Plant Resources Science, Hankyong National University)
  • 투고 : 2003.11.12
  • 심사 : 2004.01.26
  • 발행 : 2004.02.29
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초록

자외선 처리에 의한 세포 내 소기관의 구조적 변화를 관찰하기 위하여 수행하였다. 광학현미경을 통한 인산기를 가진 광합성산물은 자외선 처리시간의 증가와 함께 급격히 감소하였다. 주사전자현미경을 통한 엽 표면의 큐티클층은 자외선 처리 6시간 이후부터 파괴되기 시작하였다. 자외선에 의한 세포 수분압의 변화는 엽록체의 틸라코이드막을 파괴하였으며, 표피세포의 두께를 증가시켰는데 특히 1차 세포벽은 파괴되었으며 2차 세포벽은 리그닌의 합성 및 축적으로 인해 무처리에 비해 약 2배정도 두꺼워졌다.

This experiment was performed to observe morphological changes in rice leaf tissue caused by a successive UV-B radiation. Effect of UV-B radiation on the structural alteration of tissue was not visually found, however, Photosynthate containing phosphate was sharply reduced in proportion with an increase of UV-B radiation. Fundamental components of cuticle layer were being degraded after 6 h of UV-B radiation compared to the control. UV-B-induced mesophyll cell appeared altered because of water stress, the shape of chloroplast appeared to be considerably shrunk and chloroplast thylakoid membranes were severely destructed. Primary cell wall of UV-B-stressed tissue was entirely scattered or disappeared, and the secondary cell wall due to lignin synthesis and deposition resulted in being thickened, almost 2-times, compared with the control.

키워드

참고문헌

  1. Barnes, P. W., S. D. Flint, and M. M. Caldwell. 1990. Morphological responses of crop and weed species of different growth forms to ultmviolet-B radiation. Am. J. Bot. 77:1354-1360 https://doi.org/10.2307/2444596
  2. Bornman, J. F., R. F. Evert, and R. J. Mierzwa. 1983. The effect of UV-B and UV-C radiation on sugar beet leaves. Protoplasma 117:7-16 https://doi.org/10.1007/BF01281779
  3. Brandle, J. R., W. F. Campbell, W. B. Sisson, and M. M. Caldwell, 1977. Net photosynthesis, electron transport capacity, and ultrastructure of Pisum sativum L. exposed to ultraviolet-B radiation. Plant Physiol. 60:165-169 https://doi.org/10.1104/pp.60.1.165
  4. Cen, Y. P., and J. F. Bornman. 1993. The effect of exposure to enhanced UV-B radiation on the penetration of monochromatic and polychromatic UV-B radiation in leaves of Brassica napus. Physiol. Plantarum 87:249-255 https://doi.org/10.1111/j.1399-3054.1993.tb01727.x
  5. Lesser, M. P., and J. M. Shich. 1990. Effects of visible and ultraviolet radiation on the ultrastructure of zooxanthellae (Symbiodinium sp.) in cultures and in situ. Cell Tissue Res. 261:501-508 https://doi.org/10.1007/BF00313529
  6. Mirecki, R. M., and A. H. Teranmura. 1984. Effects of ultraviolet-B irradiance on soybean V. The dependence of plant sensitivity on the photosynthetic photon flux density during and after leaf expansion. Plant Physiol. 74:475-480 https://doi.org/10.1104/pp.74.3.475
  7. Murali, N. S., A. H. Teramura, and S. K. Randall. 1988. Response differences between two soybean cultivars with contrasting UV-B radiation sensitivities. Photochem, Photobiol. 48:653-657 https://doi.org/10.1111/j.1751-1097.1988.tb02877.x
  8. Santos, L., J. M. Almeida, and R. Salema. 1993. Plants of Zea mays L. developed under enhanced UV-B radiation. I . Some ultrastructural and biochemical aspects. J. Plant Physiol. 141:450-456 https://doi.org/10.1016/S0176-1617(11)80193-8
  9. Spurr, A. R. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastructural Res. 26:31-43 https://doi.org/10.1016/S0022-5320(69)90033-1
  10. Staxen, I., and J.F. Bornman. 1994. A morphological and cytological study of Petunia hybrida exposed to UV-B radiation. Physiol. Plantarum 91:735-740 https://doi.org/10.1111/j.1399-3054.1994.tb03013.x
  11. Teramura, A. H., M. Tevini, and W. Iwanzik. 1983. Effects of ultraviolet-B radiation on plants during mild water stress, I. Effects on diurnal stomatal resistance. Physiol. Plantarum 57:175-180 https://doi.org/10.1111/j.1399-3054.1983.tb00896.x
  12. Tevini, M. 1993. Effects of enhanced UV-B radiation on terrestrial plants, p. 125-153. In M. Tevini (ed.) UV-B radiation and ozone depletion. Effects on humans, animals, plants, microorganisms, and materials. Lewis Publishers, Boca Raton, FL, USA