Effect of Antimitotic Agent Colchicine on In Vitro Regeneration of Watermelon

  • Jaskani Muhammad J. (Institute of Horticultural Sciences, University of Agriculture) ;
  • Raza H. (Institute of Horticultural Sciences, University of Agriculture) ;
  • Khan M. M. (Institute of Horticultural Sciences, University of Agriculture) ;
  • Kwon Sung W. (Kochang Watermelon Experiment Station)
  • 발행 : 2004.12.01

초록

In vitro cultures of watermelon were treated with antimitotic agent colchicine to induce ploidy alterations, particularly the induction of tetraploids. Explants cotyledon, embryonic end of seed, transverse sections of epicotyl and hypocotyl were cultured on MS media supplemented with BA ($1{\mu}M$) and colchicine ($0.01\%,\;0.05\%\;and\;0.1\%$). Explants were subcultured on colchicine free media after 4 and 7 days. Colchicine had negative effect on in vitro regeneration but this exhibited explants related response. However, hypocotyl section of seedlings induced maximum callus on $0.01\%$ colchicine. Shoot proliferation was more in cotyledon explants cultured on colchicine ($0.01\%$) for four days. Maximum root induction and root number were recorded in embryonic end explants. Overall, cotyledon and embryonic end explants, and low colchicine concentration ($0.01\%$) was found optimal in watermelon regeneration.

키워드

참고문헌

  1. Andrus CF, Seshadri VS, Grimball PC (1971) Production of Seedless Watermelons. Agricultural Research Service, United States Department of Agriculture Technical Bull No. 1425
  2. Compton ME (1999) Dark pretreatment improves adventitious shoot organogenesis from cotyledons of diploid watermelon. Plant Cell Tiss. Org. Cult. 58: 185-188
  3. Compton ME (2000) Interaction between explant size and cultivar impacts shoot organogenic competence of watermelon cotyledons HortScience 35: 749-750
  4. Compton ME, Gray DJ (1992) Micropropagation as a mean of rapidly propagating triploid and tetraploid watermelon. Proc. Fla. State Hort. Soc. 105: 352-354
  5. Compton ME, Gray OJ (1993) Shoot organogenesis and plant regeneration from cotyledons of diploid, triploid and tetraploids watermelon, J. Amer. Soc. Hort. Sci. 118: 151-157
  6. Compton ME, Gray DJ, Elmstrom GW (1993) A simple protocol for micropropagating diploid and tetraploid watermelon using shoot-tip explants. Plant Cell Tiss. Org. Cult. 33: 211-217
  7. Dong JZ, Jia SR (1991) High efficiency plant regeneration from cotyledons of watermelon (Citrullus vulgaris Schrad.). Plant Cell Rep. 9: 559-562
  8. FAO (2004) FAO agricultural production statistics database (FAOSTAT). http://apps.fao.org/default.htm
  9. Gao SL, Zhu ON, Cai ZH, Xu DR (1996) Autotetraploid plants from colchicine-treated and culture of Salvia mi/tiorrhiza. Plant Cell Tiss. Org Cult. 47:73-77
  10. Ganga M, Chezhiyan N (2002) Influence of the antimitotic agents colchicine and oryzalin on in vitro regeneration and chromosome doubling of diploid bananas (Musa spp.). J. Hort. Sci. Biotechnol. 77: 572-575
  11. Garret JJ, Rhodes BB, Xing-Ping Z (1995) Triploid watermelons resist fruit blotch organism. Cucurbits Genet. Coop. 18: 56-57
  12. Gray DJ, Elmstrom GW (1991) Process for the accelerated production of triploid seeds for seedless watermelon cultivars. United States Patent No. 5,007,198
  13. Kihara H (1951) Triploid watermelons. Proc Amer Soc. Hort. Sci. 58: 217-230
  14. Koh GC (2002) Tetraploid production of moodeungsan watermelon. J. Kor. Soc. Hort. Sci. 43: 671-676
  15. Lower RL, Johnson KW (1969) Observations on sterility of induced autotetraploid watermelons. J. Amer. Soc. Hort. Sci. 94: 367-369
  16. Marr C, Gast KLB (1991) Reaction of consumers in a farmers market to prices for seedless watermelon and ratings of eating quality. HortTechnology 1: 105-106
  17. Maynard ON (1989) Triploid watermelon seed orientation affects seed coat adherence on emerged cotyledons. HortScience 24: 603-604
  18. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497
  19. Srivastava DR, Andrianov VM, Piruzian ES (1989) Tissue culture and plant regeneration of watermelon (Citrullus vulgaris Schrad. cv. Melitopolski). Plant Cell Rep. 8: 300-302
  20. Steel RGO, Torrie HH (1980) Principles and Procedures of Statistics. A Biometrical Approach McGraw Hill Book Company, New York
  21. SuYing T, XiuQiang H, JiWei L, WenGe L (1993) Raising the frequency of inducing tetraploid watermelon by treating of colchicine. Acta Hortic. 402: 18-22
  22. Vainola A (2000) Polyploidization and early screening of Rhododendron hybrids. Euphytica 112: 239-244