Effect of Cadium Ions on the Activity of Fungal Laccase and Its Decolorization of Dye, RBBR

  • Jarosz-Wilkolazka, A. (Department of Biochemistry, Maria Curie-Sklodowska University) ;
  • Malarczyk, E. (Department of Biochemistry, Maria Curie-Sklodowska University) ;
  • Leonowicz, A. (Department of Biochemistry, Maria Curie-Sklodowska University) ;
  • Cho, Nam-Seok (Department of Wood and Paper Science, Chungbuk National University)
  • 투고 : 2004.04.23
  • 심사 : 2004.05.26
  • 발행 : 2004.11.25

초록

The effect of cadmium ions on ligninolytic and decolourizing activities in cultures of two white-rot fungi, Cerrena unicolor and Trametes versicolor, were examined. Cadmium was added to the shallow stationary cultures growing on a liquid mineral medium. Both examined strains sorbed Cd ions in the first 24 hr of incubation. An appreciable stimulation of the activity of extracellular laccase (LAC) and inhibition of the extracellular manganese-dependent peroxidase (MnP) were simultaneously observed when 25 mgL-1 and 50 mgL-1 of cadmium ions were added to the cultures. On the other hand, the addition of cadmium ions also resulted in stimulating the decolorization activity of C. unicolor to decolorize Remazol Brilliant Blue R (RBBR) in the cultures, but decreasing it in the culture of T. versicolor, which is compared to the inhibition of MnP activity in this fungus. Our data indicate that the presence of Cd(II) ions can affect the ligninolytic activity of white-rot fungi. It was found that C. unicolor is a strain resistant to the presence of Cd ions in the liquid culture media, and has a potential to use this strain for bioremediation of sites contaminated with both heavy metals and aromatic pollutants.

키워드

과제정보

Some part of this work was supported by RTD5 EC grant No ICA2-CT-2000-10050 and by KBN grant No. 139/E-SPUB-M-5 PR-UE/DZ 280/2000, and KOFST and KOSEF Brain Pool Program (032S-4-6).