Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Anticonvulsant, Antioxidant Effect of Gungchihwadam-jeon

궁치화담전 투여가 PTZ 경련 유발시 뇌조직 중 경련 및 산화 관련물질에 미치는 영향

  • Kim Lak hyung (Department of Oriental Medicine, Woosuk University) ;
  • Kwon Bo Hyung (Department of Oriental Medicine, Woosuk University) ;
  • Lee Kwang Gyu (Department of Oriental Medicine, Woosuk University) ;
  • Oh Chan Ho (Division of Biotechnology, Woosuk University)
  • Published : 2004.02.01
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Abstract

This study was performed to evaluate the anticonvulsant, antioxidant effect of modified formulas Korean traditional medicine Gungchihwadam-Jeon(GCHDJ). The extract of GCHDJ was administered (p.o.) to mice for 14 days in anticonvulsant and antioxidant tests. The pretreatment of GCHDJ extract prohibited the pentylenetrazol(PTZ)-induced convulsion in PTZ-induced convulsion, lowered level of brain r-aminobutyric acid(GABA) was restored by the pretreatment of GCHDJ. Increased level of brain glutamic acid was lowered to normal state by GCHDJ, and increased activity of brain r-aminobutyric acid transaminase(GABA-T) was reduced by GCHDJ. In PTZ-induced convulsion, increased level of brain lipid peroxide was lowered to normal state by the pretreatment of GCHDJ. Increased activity of brain xanthine oxidase(XOD) was lowered to normal state by GCHDJ, and increased activity of brain aldehyde oxidase lowered to normal state by GCHDJ. In PTZ-induced convulsion, increased activities of superoxide dismutase(SOD) and catalase in brain were lowered by the pretreatment of GCHDJ, whereas increased level of glutathione and increased activity of gluthathione peroxidase in brain were not changed significantly. Above results suggest that GCHDJ have anticonvulsant. antioxidant effect. That seems to be strongly related with the levels of GABA, glutamate, lipid peroxide and the activities of GABA-T, XOD, aldehyde oxidase, SOD, catalase in brain tissue. From these results, GCHDJ could be applied to various convulsive disorders.

Keywords

References

  1. Current Medical Diagnosis & Treatment Lawrence M. Tieney, Jr.;Stephen J. McPhee;Maxine A. Papadakis
  2. 최신정신의학 민성길
  3. 東醫精神醫學 黃義完;金知赫(編著)
  4. 신경정신과학 대한신경정신의학회(편)
  5. 약리학 서울대학교 의과대학 약리학 교실
  6. Epilepsia v.41 no.s6 Seizure-Induced Neuronal Necrosis: Implications for Programmed Cell Death Mechanism Denson G. Fujikawa;Steve S. Shinmaei;Baiyaun Cai https://doi.org/10.1111/j.1528-1157.2000.tb01549.x
  7. J. Neurochemistry v.45 Cacium enhances in vitro free radical-induced damage to brain synaptosomes, mitochondria, and cultured spinal cord neurons Braughler J. M.;Duncan L. A.;Goodman T.
  8. Am. J. Physiol. v.235 no.5 Effect of oxygen at high pressure on sontaneous transmitter release Colton J. S.;Colton C. A. https://doi.org/10.1152/ajpcell.1978.235.5.C233
  9. J. Neurochemictry v.47 Superoxide radical-mediated alteration of synaptosome membrane structure and high affinity r-[14C] aminobutylic acid uptake Debler, E.A.;Sershen H.;Lajtha, A.;Gennaro Jr. J. F. https://doi.org/10.1111/j.1471-4159.1986.tb13092.x
  10. J. Neurochemistry v.50 Regulation of r-aminobutylic acid/Barbiturate receptor-gated chloride ion flux in brain vesicles by phospholipase A2 : Possible role of oxygen radicals Schwarz R. D.;Skolnick, P.;Paul, S. M. https://doi.org/10.1111/j.1471-4159.1988.tb02948.x
  11. 病理學 I. 대한병리학회
  12. 프리라디칼 김영곤;김영표
  13. Freeradicals in biology and medicine Barry Halliwell;John M. C. Gutteridge
  14. 黃帝內經素問校注語釋 郭靄春(編著)
  15. 編註醫學入門 李천
  16. 靑崗醫鑑 김영훈
  17. 대한한의학회지 v.20 no.1 芎梔化痰煎의 항스트레스 효과에 대한 실험적 연구 박두병
  18. Arch. Int. Pharmacodyn Anticonvulsant properties of diphenylthiohydantoin Sohn Y.J.;Levitt B.;Raines A.
  19. J. Chromatography Reversed~phase high performance liquid chromatographic method for determination of brain glutamate decarboxylase suitable for use in kinetic studies Allen I.C.;Griffthis R.
  20. Anal. Biochem. Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction Ohkawa H.;Ohishi N.;Yaki K.
  21. Blood v.55 no.5 Changes in the levels of glutathione in phagocytosing human neutrophils Alwin A.V.;Johanners A.L.;Dirk R.
  22. Method of enzymatic analysis 3eds. v.2 Bergmeyer H.U.
  23. Pediatrics v.98 no.1 Role of Xanthine oxidase and its inhibitor in hypoxia : reoxygenation injury Saugstad O.D.
  24. Biochemical pharmacology v.48 no.1 Comparion of levels of aldehyde oxidase with cytochrome P450 activies in human liver in vitro Rodriguese A.D.
  25. J. Biologocal chemistry v.244 no.22 Superoxide dismutase; an enzymic function for erythrocuprein McCord J.M;Fridovich I.
  26. Methods of enzymetic analysis Aebei H. Catalase.
  27. J. Biochemistry. v.93 Effect of bleomycin on lipid peroxides, lutathine reductase and collagenase in culture lung fibroblasts Hiraiwa K.;Oka T.;Yagi K. https://doi.org/10.1093/oxfordjournals.jbchem.a134246
  28. J. Biol. Chem. Protein measurement with the folin phenol reagent Lowry O.H.;Rodebrough N.J.;Farr A.L.;Randall R.J.
  29. 내과진단학 吉利和
  30. Mayo Clin Proc. v.76 Acute and Chronic Seizures in Patients Older Than 60 Years Joseph I. Sirven https://doi.org/10.4065/76.2.175
  31. 臨床神經診察法 田崎義昭;제藤佳雄
  32. Biochemical Pharmacology v.28 Inhibitor of GABA metabolism Brian W. Metcalf https://doi.org/10.1016/0006-2952(79)90529-X
  33. The American Journal of Chinese Medicine v.30 no.2;3 Antioxidant and Anticonvulsant Effect of a Modified Formula of Chaihu-Longu-Muli-Tang Hung-Ming Wu;Chin-San Liu;Jing-Jane Tsai;Lie-Yon Ko;Yau-Huei Wei https://doi.org/10.1142/S0192415X02000235
  34. European Journal of Neuroscience v.12 no.4 Free radical production correlates with cell death in an in vitro model of epilepsy Marina V. Frantseva;Jose L. Perez Velazquez;Paul A. Hwang;Peter L. Carlen https://doi.org/10.1046/j.1460-9568.2000.00016.x
  35. 동의신경정신과학회지 v.10 no.1 복합향(丁香, 辛夷)이 항경련효과 기전에 관한 실험실적 연구 구병수
  36. 동의신경정신과학회지 v.10 no.2 辛夷香이 mouse의 誘導痙攣에 미치는 影響 辛容玹;具炳壽
  37. 中醫症狀鑑別診斷學 中醫硏究院(主編)
  38. 實用中醫腦病學 中國中醫硏究院廣安門醫院(主編)
  39. 中醫臨床大全 楊思澍;張樹生;傳景華(主編)
  40. 實用中西醫結合診斷治療學 陳貴廷;楊思澍(主編)
  41. 中醫內科學 張伯臾
  42. 임상약리학 Bertram G. Katzung
  43. 東國大學校 博士學位論文 加減蘇合香元 香氣 및 癎寶丹이 中樞神經抑制 및 腦神經受容體 活性에 미치는 影響 辛容玹
  44. 東國大學校 博士學位論文 四物安神湯의 抗痙攣效果 및 作用機轉에 關한 實驗的 硏究 權保亨