Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Anti-oxidant Effect of the Ethyl Acetate Soluble Fraction of Sophorae Radix in H9c2 Cells

심근세포에서 고삼 에틸 아세테이트 분획의 항산화 효과

  • Kwon Kang Beom (Department of Physiology, Wonkwang University) ;
  • Kim Eun Kyung (Department of Physiology, Wonkwang University) ;
  • Lim Yang Eui (Department of Orienml Rehabilimtion Medicine, Wonkwang University) ;
  • Song Yung Sun (Department of Orienml Rehabilimtion Medicine, Wonkwang University) ;
  • Park Jong Ha (Department of Orienml Rehabilimtion Medicine, Wonkwang University) ;
  • Moon Hyung Cheal (Department of Acupuncture & Moxibustion, School of Oriental Medicine, Wonkwang University) ;
  • Ryu Do Gon (Department of Physiology, Wonkwang University)
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 김은경 (원광대학교 한의과대학 생리학교실) ;
  • 임양의 (원광대학교 한의과대학 재활의학교실) ;
  • 송용선 (원광대학교 한의과대학 재활의학교실) ;
  • 박종하 (원광대학교 한의과대학 재활의학교실) ;
  • 문형철 (원광대학교 한의과대학 침구학교실) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Published : 2004.06.01
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Abstract

To test the cytoprotective effect of sophorae radix (SR) against hydrogen peroxide (H₂O₂)-induced cytotoxicity, we investigated the cell viability using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay in the presence of ethyl acetate subfractions of SR water extracts In H9c2 cells. And to clarify the cytoprotective mechanism of SR extracts, we evaluated the cellular glutathione (GSH) contents in the presence of subfraction 1, 2, 3, and 4 of SR ethyl acetate soluble fractions. Among 1 -12 subfractions of SR ethyl acetate soluble fractions, 1, 2, 3 and 4 subfractions have an efficacy inhibiting the cytotoxicity induced by H₂O₂ in H9c2 cells. Also, the protective effects of 1, 2, 3 and 4 subfractions of SR ethyl acetate soluble fractions resulted from the anti-oxidant effects. These results suggest that ethyl acetate soluble fractions of SR water extracts is effective in the prevention of H₂O₂-induced cytotoxicity and 1, 2, 3 and 4 subfraction of ethyl acetate soluble fractions possess the anti-oxidant component.

Keywords

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