Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Comparison of Immunomodualtory Effects of Water-extracted Ginseng Radix, Pilose Asia-bell, Astragali Radix, Astractylodes Rhizoma alba and Dioscoreae Rhizoma

대표적 보기약인 인삼, 당삼, 황기, 백출, 산약 물추출액의 면역조절효과 비교

  • Shin Sang Woo (Department of Pathology, College of Oriental Medicine, Daegu Haany University) ;
  • Lee Young Sun (Department of Pathology, College of Oriental Medicine, Daegu Haany University) ;
  • Park Jong Hyun (Department of Pathology, College of Oriental Medicine, Daegu Haany University) ;
  • Kwon Taeg Kyu (School of Medicine, Keimyung University) ;
  • Suh Seong Il (School of Medicine, Keimyung University) ;
  • Kwon Young Kyu (Department of Physiology, College of Oriental Medicine, Daegu Haany University)
  • 신상우 (대구한의대학교 한의과대학 병리학교실) ;
  • 이영선 (대구한의대학교 한의과대학 병리학교실) ;
  • 박종현 (대구한의대학교 한의과대학 병리학교실) ;
  • 권택규 (계명대학교 의과대학) ;
  • 서성일 (계명대학교 의과대학) ;
  • 권영규 (대구한의대학교 한의과대학 생리학교실)
  • Published : 2004.08.01
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Abstract

This study was carried out to investigate the comparison of immunomodualtory effects of water-extracted Ginseng Radix(GR), Pilose Asia-bell(PA), Astragali Radix(AR), Astractylodes Rhizoma alba(AA) and Dioscoreae Rhizoma (DR). The parameter examined to assess apparent immunomodulatory effect of the water-extracted GR, PA, AR, AA and DR included the regulation of Nitric oxide (NO), the expression of Th1/Th2 type cytokine, the change of B cell phenotype. The water-extracted GR, PA, AR, AA and DR inhibited NO production and iNOS protein expression in LPS stimulated RAW 264.7 macrophage cells. In the Th1 and Th2 cytokine expression, the water-extracted GR, PA, AR, AA and DR induced IL-2 and IFNr mRNA gene expression. Therefore, it seems that the water-extracted GR, PA, AR, AA and DR have a inducing effect of Th1 type cytokines. In the Flow cytometry analysis, the water-extracted GR, PA, AR, AA and DR did not change B cell phenotype (CD45R/B220). The water-extracted GR, PA, AR, AA and DR have a reducing effect of immune suppression cause by Methotrexate (MTX), an agent of immune suppression. These results suggest that the immunomodulatory effects of the water-extracted GR, PA, AR, AA and DR may be, in part, associated with the inducing IL-2 and IFNr mRNA gene expression in and regulation of NO production in macrophage cells.

Keywords

References

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