Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Gene Expression Profile of Rat Hypothalamus Treated with Electroacupuncture at ST36 Acupoint

족삼리 전침자극에 의한 흰쥐 hypothalamus의 유전자 발현 profile 분석

  • Rho Sam Woong (Department of Physiology, College of Oriental Medicine, KyungHee University) ;
  • Lee Gi Seog (Department of East-West Medicine, Graduate School, Kyunghee University) ;
  • Choi Gi Soon (Department of East-West Medicine, Graduate School, Kyunghee University) ;
  • Na Young In (Purimed R&D Institute) ;
  • Hong Moo Chang (Department of Physiology, College of Oriental Medicine, KyungHee University) ;
  • Shin Min Kyu (Department of Physiology, College of Oriental Medicine, KyungHee University) ;
  • Min Byung il (Department of East-West Medicine, Graduate School, Kyunghee University) ;
  • Bae Hyun Su (Department of Physiology, College of Oriental Medicine, KyungHee University, Purimed R&D Institute)
  • 노삼웅 (경희대학교 한의과대학 생리학교실) ;
  • 이기석 (경희대학교 대학원 동서의학과) ;
  • 최기순 (경희대학교 대학원 동서의학과) ;
  • 나영인 (퓨리메드㈜ 기업부설연구소) ;
  • 홍무창 (경희대학교 한의과대학 생리학교실) ;
  • 신민규 (경희대학교 한의과대학 생리학교실) ;
  • 민병일 (경희대학교 대학원 동서의학과) ;
  • 배현수 (경희대학교 한의과대학 생리학교실, 퓨리메드㈜ 기업부설연구소)
  • Published : 2004.08.01
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Abstract

Electroacupuncture (EA) has been reported to increase pain threshold, and to enhance the NK cell activity by up-regulation of IFN-γ and endogenous β-endolphin. For the purpose of understanding the molecular mechanism of EA stimulation, we analyzed the gene expression profile of rat hypothalamus, treated on Zusanli (ST36) with EA, in comparison with control group by oligonucleotide chip microarray (Affymetrix GeneChip Rat Neurobiology U34 Array) and real-time RT-PCR. Sprague-Dawley (S-D) male rats were stimulated at the Zusanli (ST36) acupoint in restriction holder. Simultaneously the control group was given only holder stress without EA stimulation. In order to prove the appropriateness of EA treatment, we measured spleen NK cell activity with standard 51Cr release assay. NK cell activity of EA group was significantly increased comparing to control group. The microarray and PCR results show that EA treatment up-regulates expression of genes associated with 1) nerve growth such as NGF induced factor A and VGF, 2) signal transduction such as 5HT3 receptor subunit, AMPA receptor binding protein and Na-dependent neurotransmitter transporter, and 3) anti-oxidation such as superoxide dismutase and glutathione S-transferase. In addition, the activity of the anti-oxidative enzyme, SOD of hypothalamus, liver and RBC was enhanced compared to that of control. The list of differentially expressed genes may implicate further insight on the mechanism of acupuncture effects.

Keywords

References

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