Effects of Methanol Extract of Radix Achyranthis Bidentatae on Cadmium Inhalation Toxicity in Rat

우슬 메탄올 추출물이 흰쥐의 카드뮴 흡입독성완화에 미치는 영향

  • Kang Hong Gu (Department of Oriental Medicine, Graduate School, Wonkwang University) ;
  • Hong Ji Woo (Department of Oriental Medicine, Graduate School, Wonkwang University) ;
  • Han Hyun Jung (Department of Oriental Medicine, Graduate School, Wonkwang University) ;
  • Hwang Yoo Yeon (Department of Oriental Medicine, Graduate School, Wonkwang University) ;
  • Jeong Jae Yeal (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Ki Nam (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 강홍구 (원광대학교 대학원 한의학과) ;
  • 홍지우 (원광대학교 대학원 한의학과) ;
  • 한현정 (원광대학교 대학원 한의학과) ;
  • 황유연 (원광대학교 대학원 한의학과) ;
  • 정재열 (원광대학교 한의학전문대학원 제3의학과) ;
  • 이기남 (원광대학교 한의학전문대학원 제3의학과)
  • Published : 2004.12.01

Abstract

To study the effects between Cd inhalation toxicity and methanol extract of Radix Achyranthis Bidentatae, 4 rat groups were exposed to Cd aerosol by whole-body inhalation exposure for 6 hours/day, 5 days/week, and 4 weeks. Cd concentration in air was 0.98㎎/㎥ and mass median diameter(MMD) was 1.78㎛. 3 different dose intraperitoneal injections of methanol extract of Radix Achyranthis Bidentatae to 3 inhalation exposure groups applied for 4 weeks and the results were as follows: The highest body weight gain for 4 weeks and food intake per day were from inhalation exposure group Ⅲ(p<0.05). The highest lung weight was from inhalation exposure group Ⅲ and the highest liver and kidney weight were from inhalation exposure group Ⅱ(p<0.05). The lowest Cd content in lung was 22.77㎍/g from inhalation exposure group Ⅲ(p<0.05). The highest Cd concentration in blood was 11.71㎍/㎗ from inhalation exposure group Ⅰ(p<0.05). Cd concentrations of 14.87㎍/g in liver and 17.91㎍/g in kidney were the highest from inhalation exposure group Ⅰ(p<0.05). The lowest Cd concentration in liver and kidney were 5.71㎍/g and 3.17㎍/g from the control(p<0.05). For weekly Cd concentration in urine, the highest value was 0.48㎍/㎖ from inhalation exposure group Ⅲ of the 3rd week and inhalation exposure group Ⅰ, Ⅱ of the 4th week. For weekly Cd concentration in feces, the highest value was 0.32㎍/g from inhalation exposure group Ⅰ, Ⅱ, Ⅲ. The highest metallothionein concentration in lung was 89.02㎍/g from inhalation exposure group Ⅲ(p<0.05). The highest metallothionein concentrations in liver and kidney were 265.47㎍/g and 214.21㎍/g from inhalation exposure group Ⅲ, respectively(p<0.05). The highest Hct, Hb, and WBC values were from inhalation exposure group Ⅱ and the highest RBC value was from inhalation exposure group Ⅲ(p<0.05). Mostly damaged part in liver tissue was hepatic lobule and the degrees of damage were lessened by the intraperitoneal injection of methanol extract of Radix Achyranthis Bidentatae. Proximal, distal convoluted tubules and glomerulus in kidney tissue were mostly damaged part. Degeneration and swelling were partially observed but the degrees of kidney tissue damage were lessened more or less by the intraperitoneal injection of methanol extract of Radix Achyranthis Bidentatae.

Keywords

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