Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Methanol Extract of Radix Achyranthis Bidentatae on Cadmium Inhalation Toxicity in Rat

우슬 메탄올 추출물이 흰쥐의 카드뮴 흡입독성완화에 미치는 영향

  • Kang Hong Gu (Department of Oriental Medicine, Graduate School, Wonkwang University) ;
  • Hong Ji Woo (Department of Oriental Medicine, Graduate School, Wonkwang University) ;
  • Han Hyun Jung (Department of Oriental Medicine, Graduate School, Wonkwang University) ;
  • Hwang Yoo Yeon (Department of Oriental Medicine, Graduate School, Wonkwang University) ;
  • Jeong Jae Yeal (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Ki Nam (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 강홍구 (원광대학교 대학원 한의학과) ;
  • 홍지우 (원광대학교 대학원 한의학과) ;
  • 한현정 (원광대학교 대학원 한의학과) ;
  • 황유연 (원광대학교 대학원 한의학과) ;
  • 정재열 (원광대학교 한의학전문대학원 제3의학과) ;
  • 이기남 (원광대학교 한의학전문대학원 제3의학과)
  • Published : 2004.12.01
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Abstract

To study the effects between Cd inhalation toxicity and methanol extract of Radix Achyranthis Bidentatae, 4 rat groups were exposed to Cd aerosol by whole-body inhalation exposure for 6 hours/day, 5 days/week, and 4 weeks. Cd concentration in air was 0.98㎎/㎥ and mass median diameter(MMD) was 1.78㎛. 3 different dose intraperitoneal injections of methanol extract of Radix Achyranthis Bidentatae to 3 inhalation exposure groups applied for 4 weeks and the results were as follows: The highest body weight gain for 4 weeks and food intake per day were from inhalation exposure group Ⅲ(p<0.05). The highest lung weight was from inhalation exposure group Ⅲ and the highest liver and kidney weight were from inhalation exposure group Ⅱ(p<0.05). The lowest Cd content in lung was 22.77㎍/g from inhalation exposure group Ⅲ(p<0.05). The highest Cd concentration in blood was 11.71㎍/㎗ from inhalation exposure group Ⅰ(p<0.05). Cd concentrations of 14.87㎍/g in liver and 17.91㎍/g in kidney were the highest from inhalation exposure group Ⅰ(p<0.05). The lowest Cd concentration in liver and kidney were 5.71㎍/g and 3.17㎍/g from the control(p<0.05). For weekly Cd concentration in urine, the highest value was 0.48㎍/㎖ from inhalation exposure group Ⅲ of the 3rd week and inhalation exposure group Ⅰ, Ⅱ of the 4th week. For weekly Cd concentration in feces, the highest value was 0.32㎍/g from inhalation exposure group Ⅰ, Ⅱ, Ⅲ. The highest metallothionein concentration in lung was 89.02㎍/g from inhalation exposure group Ⅲ(p<0.05). The highest metallothionein concentrations in liver and kidney were 265.47㎍/g and 214.21㎍/g from inhalation exposure group Ⅲ, respectively(p<0.05). The highest Hct, Hb, and WBC values were from inhalation exposure group Ⅱ and the highest RBC value was from inhalation exposure group Ⅲ(p<0.05). Mostly damaged part in liver tissue was hepatic lobule and the degrees of damage were lessened by the intraperitoneal injection of methanol extract of Radix Achyranthis Bidentatae. Proximal, distal convoluted tubules and glomerulus in kidney tissue were mostly damaged part. Degeneration and swelling were partially observed but the degrees of kidney tissue damage were lessened more or less by the intraperitoneal injection of methanol extract of Radix Achyranthis Bidentatae.

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References

  1. Casarett and Doulls Toxicology : The Basic Science of Poisons Toxic effects of metals Goyer, R. A.;C. D. Klaassen(Ed.)
  2. Industrial Toxicology: Safety and Health Applicatons in the Workplace Williams P.L.;Burson J.L.
  3. Goodman and Gilman's The Pharmacological Basis of Therapeutics Hardman, J.G.;Mimbird, L.E.
  4. Handbook on the Toxicology of Metals Cadmium Friberg, L.;Kjellstrom, T.;Nordberg, G.;L. Friberg;G.F. Nordberg; V. Vouk(Eds.)
  5. Toxicol. Appl. Pharmacol. v.54 Rapid Solubilization and Translocation of Cadmium-109 Oxide Following Pulmonary Deposition Hadley, J.G.;C.L. Sanders;A.W. Conklin
  6. Environ. Res. v.26 In vivo Measurement of Liver and Kidney Cadmium in Worker Exposed to This Metal: Its Significance with Respect to Cadmium in Blood and Urine Roe1s, H.A.;Lauwerys, R.R.;Bucket, J.P.;Bernard, A.;Chettle, D.C.;Harvey, T.C.;Al-Hadded, I.K.
  7. Toxicol. Appl. Pharmacol. v.63 Decreased effectiveness of chelation therapy for Cd poisoning with time Cantilena, L.R.;Klaasssen, C.D.
  8. Goodman and Gilman's The Pharmacological Basis of Therapeutics Gilman, A.G.;Goodman, L.S.;Rall, T.W.;Murad, F.
  9. Int. Prog. Chemical Safety Cadmium, Environmental Health Criteria 134 WHO
  10. 동의생리병리학회지 v.16 no.1 폐葉 추출물이 카드뮴에 흡입폭로된 랫드의 독성에 미치는 영향 강성호;천병렬;김상덕;송용선;이기남;정재열
  11. 동의생리병리학회지 v.16 no.6 桑葉 추출물이 카드뮴에 흰쥐의 카드뮴 에어로졸 흡입독성에 미치는 영향 박재수;정재열;이택준;강성호;송용선;이기남
  12. 魚腥草 분획이 흰쥐 장기내 카드뮴 축적과 Metallothionein 생성에 미치는 영향(VI) v.22 no.1 이기남;정재열;송용선;이정호;유일수;김신기;백승화
  13. 임상 본초학 신민교
  14. Chinese Herbal Medicine Materia Medica Bensky, D.;Gamble, A.
  15. 동의생리병리학회지 v.18 no.2 牛膝 추출물이 카드뮴 흡입폭로된 흰쥐의 독성해독에 미치는 영향 김홍기;정재열;박승정;강성호;송용선;이기남
  16. 동의생리병리학회지 v.16 no.5 카드뮴 흡입독성연구를 위해 설계된 에어로졸 발생장치에서 발생된 카드뮴 에어로졸의 입경분석 정재열;도날드 밀턴;김태형;이종영;장두섭;강성호;송용선;이기남
  17. 동의생리병리학회지 v.17 no.3 桑葉 Ethyl Acetate 추출물이 카드뮴의 흡입독성에 미치는 영향 정재열;송용선;이기남
  18. Am. Ind. Hyg. Assoc. J. v.47 no.12 Acute Inhalation Study in the Rat of Comparative Uptake, Distribution and Excretion for Different Cadmium Containing Materials Rusch, G.M.;O'Grodnick, J.S.;Rinehart, W.E.
  19. Aerosol Technology: Properties, Behavior, and Measurement of Airborne Particles Hinds, W.C.
  20. Method 8005 NIOSH Manual of Analytical Methods (NMAM) Eller, P.M.
  21. Methods Emzymol v.205 Determination of metallothionein in tissue by cadmium-hemoglobin assay Eaton DL;Cherian MG.
  22. Toxicology and Applied Pharmacology v.161 Attenuation of Cadmium- Induced Liver Injury in Senescent Male Fischer 344 Rats: Role of Metallothionein and Glutathione Tetsuo, Y.;Kosanke, S.D.;Rikans, L.E.
  23. Shoyakugaku Zasshi v.35 Plant growth regulating activities of crude drugs and medicinal plants Shimomura, H.;Sashida, Y.;Nakata, H.
  24. Documentation of the Threshold Limit Values and Biological Exposure Indices American Conference of Governmental Industrial Hygienists
  25. Toxicology Principles and Applications Niesink, R.J.M.;Vries, J.D.;Hollinger, M.A.
  26. Handbook of Human Toxicology Massaro, E.J.
  27. Toxicol. Appl. Pharmacol. v.34 Studies on cadmium effects in living organisms-impairment due to inhalation Yoshikawa, H.;Kawai, K.;Suzuki, Y.;Nozaki, K.;Ohsawa, M.
  28. 한국영양학회지 v.29 no.6 식이내 Cysteine 수준이 흰쥐의 카드뮴과 납중독에 미치는 영향 류정미;김미경
  29. International Commission on Radiological Protection no.23 Report of the Task Gruop on Reference Man Snyder, W.S.
  30. Cadmium Studies in Japan: A Review Tsuchiya, K.
  31. Toxicology v.116 Colchicine- induced elevation of tissue metallothionein contents is mediated by inflammation-independent serum factor Itoh, N.;Kasmatsu, M.;Onosaka, S;Muto, N.;Tanaka, K.
  32. Toxicol. Lett. v.84 Cadmium accumulation and metallothionein expression in brain of mice at different stage of development Choudhuri, S.;Liu, W.L.;Berman, N.E.J.;Klaassen, C.D.
  33. Toxicol. Appl. Pharmacol. v.107 The protective effect of metallothionein on the toxicity of various metals in rat primary hepatocyte culture Liu, J.;Kershaw, W.C.;Klaassen, C.D.
  34. Fresenius J Anal Chem v.368 Quantification of the metal distribution in metallothioneins of the human liver by HPLC coupled with ICP-AES Wolf, C.;Rosick, U.;Bratter, P.
  35. Toxicol. Appl. Pharmacol. v.101 Pulmonary tolerance to cadmium following cadmium aerosol pretreatment Hart, B. A.;Voss, G. W.;Willean, C. L.
  36. Toxicol Appl Pharmacol. Kidney synthesizes less metallo- thionein than liver in response to cadmium chloride and cadmium metallothionein Sendelbach, L.E.;Klaassen, C.D.
  37. Comparative Biochemistry and Physiology v.126 Dietary cadmium induces histological changes despite a sufficient metallo- thionein lever in the liver and kidneys of the bank vole(Clethrionomys glareolus) Wlostowski, T.;Krasowska, A.;Laszkiewicz-Tiszczenko, B.
  38. Arch. Toxicol. v.65 Cadmium/zinc- metallothionein induces DNA strand breaks in vitro Muller, T.;Schuckelt, R.;Jaenicke, L.
  39. J. Trace Elem. Exp. Med v.11 Cadmium-induced renal dysfunction: new mechanism, treatment and prevention Nomiyama, K.;Nomiyama, H.
  40. Toxicol. Appl. Pharmacol. v.68 Minimal role of metallothionein in decreased chelator efficiency for cadmium Waalkes, M.P.;Watkins, J.B.;Klaassen, C.D.