대한한의학회지 (The Journal of Korean Medicine)
- 제25권4호
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- Pages.15-25
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- 2004
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- 1010-0695(pISSN)
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- 2288-3339(eISSN)
Effects of Bambusae concretio Silicea on Suppression of Collagenolysis and Bone Resorption in Mouse Calvarial Osteoblasts
- Lee Seong-Choon (Department of Oriental Medicine, College of Oriental Medicine, Dongguk University) ;
- Yoon Cheol-Ho (Department of Oriental Medicine, College of Oriental Medicine, Dongguk University) ;
- Jeong Ji-Cheon (Department of Oriental Medicine, College of Oriental Medicine, Dongguk University)
- 발행 : 2004.12.01
초록
Objective : We studied the effect of Bambusae concretio Silicea (BCS) on bone metabolism. Methods : At first, we treated PTH, 1,25(OH)₂D₃, mononuclear cell conditioned medium (MCM) and IL-1 to osteoblast cells derived from mouse calvarial bone explants in vitro, and then investigated the activities of collagenolysis and bone resorption factors. Results : BCS extracts have no cytotoxicities in concentrations of 1-150 ㎍/ml. BCS had protective activity against PTH (5 units/ml), MCM (5%, v/v), 1,25(OH)₂D₃ (20 ng/ml), IL-1α(2 ng/ml) and IL-1β, (1 ng/ml)-induced collagenolysis in the mouse calvarial cells. And, pretreatment of BCS for 1 hr significantly reduced the collagenolysis. Furthermore, it was much more expressed at 16 hrs after BCS (50 ㎍/ml)-pretreatment. And, BCS significantly protected against enhanced collagenolysis induced by IL-1α and IL-1β. Conclusion : BCS extracts inhibited the bone resorption in mouse calvarial bone cell;, thus BCS could be used clinically for bone diseases.