Reproductive and Developmental Biology

  • 제28권4호
  • /
  • Pages.227-233
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  • 2004
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

Effects of Various Addition and Exclusion Time of Glucose on Development of Mouse Two-Cell Embryos

  • Park S. B. (Department of Animal Science, Daegu University) ;
  • Park K S. (Department of Obstetrics and Gynecology, Kyungpook National University Hospital) ;
  • Lee T. H. (Department of Obstetrics and Gynecology, Kyungpook National University Hospital) ;
  • Chun S. S. (Department of Obstetrics and Gynecology, Kyungpook National University Hospital) ;
  • Kim K S. (Cheonan Yonam College) ;
  • Song H. B. (Department of Animal Science, Daegu University)
  • 발행 : 2004.12.01
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초록

This study was conducted to investigate the effect of various addition and exclusion time of glucose (Control: no addition, A: 24～72 h, B: 24～48 h, C: 48～72 h, D: 0～72 h, E: 0～48 h, F: 0～24 h and 48～72 h, G: 0～24 h) on embryonic developmental capacity of 2-cell embryos in mice. Developed blastocysts were assessed for mean cell number by differential staining. The zona-intact blastocyst (ZiB) rates were higher (p<0.05) in group B than control. However, the zona-escape blastocyst (ZeB) rates were not significantly different in all groups. At 72 h, total blastocyst (ZiB + ZeB) formation rates were not significantly different in all groups. The mean cell number was not significantly different among all groups. The inner cell mass (ICM) cell number was higher (p<0.05) in group F than control, group A, B and G. The trophectoderm (TE) cell number was higher (p<0.05) in control than group A and D. The %ICM was higher (p<0.05) in group C, D and F than control. The ICM : TE ratio was not significantly different in all groups. Between control and glucose group, no significant difference was observed in the total blastocysts (ZiB + ZeB) formation rates. Also, no significant difference was observed in the mean cell number, ICM cell number and ICM : TE ratio. However the TE cell number was higher (p<0.05) in control than glucose group and %ICM was higher (p<0.05) in glucose group than control. In conclusion, glucose added in culture medium was not inhibitory on blastocyst formation but glucose added for 48 ～72 h in culture medium increases %ICM of blastocysts in mice.

키워드

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