YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effects of Caffeic Acid Methyl Ester from Mentha arvensis Linne var. piperascens on Function of E6 Oncoprotein of HPV 16

박하에서 분리한 Caffeic Acid Methyl Ester가 자궁경부암 바이러스 발암단백질 E6의 기능에 미치는 영향

  • Baek, Tae-Woong (Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee , Kyung-Ae (Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn , Min-Jung (Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology) ;
  • Joo, Hae-Hyung (Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology) ;
  • Oh , Hyun-Cheol (Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn , Jong-Seok (Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology) ;
  • Cho , Yong-Kweon (Department of Healthscience and Biochemistry, Changwon National University) ;
  • Myung , Pyung-Keun (Department of Pharmacy, College of Pharmacy Chungnam National University) ;
  • Yoon, Do-Young (Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology)
  • 백태웅 (한국생명공학연구원 세포생물학연구실) ;
  • 이경애 (한국생명공학연구원 세포생물학연구실) ;
  • 안민정 (한국생명공학연구원 세포생물학연구실) ;
  • 주혜경 (한국생명공학연구원 세포생물학연구실) ;
  • 오현철 (한국생명공학연구원 세포생물학연구실) ;
  • 안종석 (한국생명공학연구원 세포생물학연구실) ;
  • 조용권 (창원대학교 보건생화학과) ;
  • 명평근 (충남대학교 약학과) ;
  • 윤도영 (한국생명공학연구원 세포생물학연구실)
  • Published : 2004.01.01
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Abstract

Cervical cancer is one of the leading causes of female death. Viral oncoproteins E6 and E7 are selectively retained and expressed in carcinoma cells infected with HPV (Human pa pilloma virus) type 16 and cooperated in immotalization and transformation of primary keratinocyte. E6 and E7 oncoproteins interfere the functions of tumor suppressor proteins p53 and retinoblasoma protein (pRb), respectively. Among a lots of natural products, Mentha arvensis Linne var.piperascens have inhibitory effects on bindings between E6 oncoprotein and tumor suppressor p53, E3 ubiqutin- protein ligase (E6AP). HPV oncoprotein inhibitors from Mentha piperita L. were isolated by solvent partition and column chromatography (Silica gel, RP-18) and inhibitory compounds were finally purified by HPLC using an ELISA screening system based on binding between E6 and E6AP. The aim of this study is to identify the structure of inhibitory compounds and to investigate whether these compounds have inhibitory effects on functions of E6 oncoprotein. We investigated whether caffeic acid methyl ester (CAM) extracted from Mentha piperita L. could inhibit the function of E6 oncoprotein. CAM inhibited the in vitro binding of E6 and E6AP which are essential for the binding and degradation of the tumor suppressor p53 and also inhibited the proliferation of human cervical cancer cell lines (SiHa and CaSKi) in a dose response manner. These results suggest that CAM inhibited the function of E6 oncoprotein, suggesting that it can be used as a potential drug for the treatment of cervical cancers infected with HPV.

Keywords

References

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