축산제품에 응용하기 위한 콩제품 추출물의 항산화 능력에 관한 연구

Study on the Antioxidant Activity of Soybean Products Extracts for Application of Animal Products

  • 이치호 (건국대학교 축산대학 축산가공학과) ;
  • 문성용 (건국대학교 축산대학 축산가공학과) ;
  • 이중칠 (건국대학교 축산대학 축산가공학과) ;
  • 이지영 (건국대학교 축산대학 축산가공학과)
  • 발행 : 2004.12.01

초록

본 연구는 콩 제품 추출물의 항산화 활성을 DPPH 수소 공여 능으로 실험하고, 기존의 항산화제와의 상승효과 및 TBA radical scavenging activity를 실험하였고 그 결과는 다음과 같다. 콩 제품 추출물은 DPPH와 반응하여 시간이 경과함에 따라 감소하고, 시판 항산화제인 ascorbic acid는 반응 초기에 완료되는 결과를 보이는 반면 BHT와 BHA는 지속적으로 감소되는 것을 확인할 수 있었다. 또한 콩 발효식품인 된장과 청국장 추출물이 비 발효식품인 두유 추출물에 비하여 감소폭이 다소 크게 차이가 나타났다. 콩제품 추출물과 시판 항산 화제의 상승효과를 조사한 결과 모든 시험 구에서 반응시간이 경과함에 따라 감소폭이 증가하여 상승효과가 나타났으며 발효 식품인 된장과 청국장이 비 발효 식품인 두유에 비하여 시판 항산화제와의 상승효과를 더욱 강하게 나타내었다. 콩 제품 추출물의 TBA 측정을 조사한 결과, 청국장 추출물의 억제율은 45.8%, 된장추출물의 억제율은 45.2%로 나타났고, 두유 추출물의 억제율은 이보다 약 12% 낮은 33.6%로 나타났다. 따라서 이들 추출물 중 두유 추출물은 기존의 이 소플라본이 생체에 미치는 긍정적인 효과와 함께 이들 항산화제 기능을 축산식품에 응용 가능한 것으로 사료된다.

This study was performed to investigate the antioxidative activity of soybean products extracts using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and thiobarbituric acid (TBA) method for application of animal products. All three extracts (natto, soybean paste, soybean milk extract) were found to have an ability to donate hydrogen to DPPH. Especially, natto and soybean paste extract were more effective than that of soybean milk on the antioxidative activities. Natto and soybean paste extract were stronger than butylhydroxy toluene (BHT), butyl hydroxyl anisole (BHA) (5${\times}$10$^{-4}$ M) and ascorbic acid (5.7${\times}$10$^{-3}$ M) previously well known as antioxidants. These extracts also showed a synergistic effect. TBA values of natto (45.8%) and soybean paste extract (45.2%) were stronger than that of soybean milk extract (33.6%). These results suggest that soybean product extracts have antioxidative activities and synergistic effects.

키워드

참고문헌

  1. Branen, A, L. (1975) Toxicology and biochemistry of buty-lated hydroxyl anisole and butylated hydroxyl toluene. Am. J. Oil Chem. Soc. 52, 59-62 https://doi.org/10.1007/BF02901825
  2. Chi, Hee Youn (1999) The study of physical characteristics and antioxidative activity and isoflavones analysis on collected soybean (Glycine max (L.) Merrill). J. Kor. Soc. of Med. Crop Sci. 45(1), 26-28
  3. Choi, Y. B. and Sohn, H. S. (1998) Isoflavone content in Korean fermented and unfermented soybean foods. Korean J. Food Sci. Technol. 30, 745-750
  4. Choi, Y. B., Woo, J. G., and Noh, W. S. (1999) Hydrolysis of $\beta$-glycosidic bonds of isoflavone conjugates in the lactic acid fermentation of soy milk. Korean J. Food Sci. Technol. 31, 189-195
  5. Coward, L, Smith, M., Kirk, M., and Bames, S. (1998) Chemical modification of isoflavones in soyfoods during cooking and processing. Am. J. Clin. Nutr. 68, 1486S-1491S
  6. Esaki, H., Onozaki, H., Morimitsu, Y., Kawakishi, S., and Osawa, T. (1998) Potent antioxidative isoflavones isolates from soybeans fermented with Aspergillus saitoi. Bio Sci. Biotechnol and Biochem. 62(4), 740-746 https://doi.org/10.1271/bbb.62.740
  7. Fukutakc, M., Takahashi, M., Ishida, K., Kawamura, H., Sugimura, T., and Wakabayashi, K. (1996) Quantification of genistein and genistin in soybeans and soybean products. Food and Chemical Toxicology 34, 457-461 https://doi.org/10.1016/0278-6915(96)87355-8
  8. Low, G., S. H., and Custer, L. J. (1999) Isoflavone levels in soy foods consumed by multiclinic populations in Singa-pore and Hawaii. J. Agric. Food Chem. 47, 977-986 https://doi.org/10.1021/jf9808832
  9. Gyorgy, P., Murata, K., and Ikehara, H. (1964) Antioxidants isolated from fermented soybeans (Tempeh). Natwe 203, 870-87
  10. Ha, E. Y. W., Morr, C. V., and Seo, A. (1992) Isoflavone aglycones and volatile organic compounds in soybeans: effects of soaking treatment. J. Food Sci. 57, 414-417 https://doi.org/10.1111/j.1365-2621.1992.tb05506.x
  11. Hahm, T. S., King, D. I., and Min, D. B. (1993) Food antioxidants research. Food and Biotechnol 30(1), 14-21
  12. Halliwell, B. and Chirico, S. (1993) Lipid peroxidation : Its mechanism, measurement and significance. Am. J. CIin. Nutr. 57, 715S-717 S
  13. Harbome, J. R. (1994) The ftavonoids: advances m research since 1986. Chapman and Hall, NY, pp. 676
  14. Izumi, T., Piskura, M. K., Osawa, S., Obata, A., Tobe, K., Saito, M., Kataoka, S., Kubota, Y., and Kikuchi, M. (2000) Soy isoflavone aglycones are absorbed faster and in higher amounts than glucosides in humans. J. Nutr. 13, 1695-1699
  15. Lee, J. W., Do, J. H., and Shim, K. H. (1999) Antioxidant activity of the water soluble browning reaction products isolated from Korean Red Ginseng. J. Ginseng Res. 23(3), 176-181
  16. Kim, J. S. and Yoon, S. (1999) Isoflavone content and $\beta$-glucosidase activities of soybeans, meju, and doenjang. Korean J. Food Sci. Technol. 31, 1405-1409
  17. Mahungu, S. M., Diaz-Mercado, S., Li, J., Schwenk, M., Singletary, K., and Faller, J. (1999) Stability of isoflavones duhng extrusion processing of com/soy mixture. J. Agric. Food Chem. 47, 279-284 https://doi.org/10.1021/jf980441q
  18. Matsuura, M. and Obata, A. (1993) $\beta$-Glucosidases from soybeans hydrolyze daidzin and genistin. J. Food Chem. 58, 144-147 https://doi.org/10.1111/j.1365-2621.1993.tb03231.x
  19. Reinli, K. and Block, G. (1996) Phytoestrogen content of foods - a compendium of literature values. Nutr. Cancer 26, 123-148 https://doi.org/10.1080/01635589609514470
  20. Reramoto, R., Y., Ohta, N., and Ueda, S. (1994) Solubili-zation of a novel isoflavone glycoside-hydrolyzmg $\beta$-gluco-sidase from Lactobaxittus casei subsp. rhamnouse. J. Fer-ment. Bioeng. 11, 493-441
  21. SAS (1988) SAS/STAT Software for PC. Release 6.03, SAS Institute Inc., Cary, NC, USA
  22. Setchell, K. D. R., Brown, N. M., Desai, P., Zimmer-Nechemias, L., Wolfe, B. E., Brashear, W. T., Kirschner, A. S., Cassidy, A., and IIeubi, J. E. (2001) Bioavailability of pure isoflavones in healthy humans and analysis of com-mercial soy isoflavones supplements. J. Nutr. 131, 1362S-1375S
  23. Shin, D. H. (1997) The trend and direction of natural antioxidants research. Food Sci. and Industry 30(1), 14-21
  24. Wang, H. J. and Murphy, P. A. (1994) Isoflavone content in commercial soybean foods. J. Agric. Food Chem. 42, 1666-1673 https://doi.org/10.1021/jf00044a016
  25. Wang, H. J. and Murphy, P. A. (1996) Mass balance study of isoflavones during soybean processing. J. Agric. Food Chem. 44, 2377-2383 https://doi.org/10.1021/jf950535p
  26. Williamoson, G., Plumb, G. W., Uda, Y., Price, K. R., and Rhides, M. J. C. (1996) Dietary quercetin glycosides: antioxidant activity and induction of the anticarcinogenic phaseII marker enzyme quinone reductase in Hepaicic7 cells. Carcinogenesis 17, 2358-2387
  27. Williamoson, G., Plumb, G. W., Uda, Y., Price, K. R., and Rhides, M. J. C. (1996) Dietary quercetin glycosides: antioxidant activity and induction of the anticarcinogenic phase II marker enzyme quinone reductase in Hepaicic7 cells. Carcinogenesis 17, 2358-2387
  28. Wu, J. (1994) Isoflavone content in isolated soy protein products. MS. thesis, Univ. of Illinois, Urbana, IL