Plant Resources
- Volume 7 Issue 3
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- Pages.200-205
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- 2004
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- 1226-8771(pISSN)
Elimination of SPFMV from Virus-infected Sweet Potato Plants through Apical Meristem Culture
- Kim, Young-Seon (Dept. of The Development of Medicinal Resources and Horticulture, Namdo Provincial College of Jeonnam) ;
- Jeong, Jae-Hun (Division of Forest Genetics Resources, Korea Forest Research Institute) ;
- Park, Jong-Suk (Jinan Medicinal Herbs Experiment Station) ;
- Eun, Jong-Seon (Dept. of Horticulture, Chonbuk National University)
- Published : 2004.12.01
Abstract
Sweet potato infected with a viral disease (SPFMV) showed irregular chlorotic patterns, so called feathering associated with faint or distinct ring spots that have purple-pigmented borders. SPFMV was eliminated from sweet potato plants using meristem tip culture. MS medium supplemented with BAP (2mg/L) and NAA (0.05 mg/L) was used for shoot proliferation and 1/2 MS medium for rooting of the plants. Highest percentage of regenerated plants (60%) was obtained from the optimum size (0.3-0.5mm) meristem tips. Of these, 60% plants were found negative for SPFMV by RT-PCR. Virus detection by RT-PCR was found to be a reliable method. Meristem-tip culture to produce SPFMV-free quality sweet potato and virus detection by RT-PCR is an efficient, time saving and reliable method for production of SPFMV-free tissue culture raised plants.