Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
권호 표지

Effects of Ixeris dentata Extracts on the Genotoxicity Induced by Gamma Irradiation in Rats

  • Published : 2004.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Ixeris dentata is a typical oriental herb. It is a widely distributed perennial in Korea, Japan and China, which belongs to the Compositae Family. The whole plant of I. dentata has been used for the treatment of pneumonia, contusion, tumor and hepatitis. It has also been used for the treatment of allergic diseases as a folk therapy in Korea. I. dentata is known to have aliphatics, triterpenoids and sesquiterpene glycosides in its composition. The present study was designed to explore the protective effects of water- and ethanol- extracts from I. dentata on irradiated rodents. For oral administration (twice per day), the extractive powder of I. dentata and the positive control (ascorbic acid) were dissolved at a concentration of 0.5 and 250mg $ml^{-1}$ in saline, respectively. Thirty days after irradiation, the ratio of the weight of the testis to the body weight was lower than 50% in the radiation groups than the control group. The ALP concentrations in the group treated with the water-extracts of the leaf were $79.68\pm{1.39%}$ (p<0.05) of those of the radiation control. Both of the SGOT and SGPT in the group treated with the ethanol-extract of the root were $72.68\pm{0.95}\;and\;77.87\pm{5.74}$ (p<0.05) of those of the radiation control, respectively. The levels of DNA damage induced by gamma radiation decreased in the experimental group to which the extracts of I. dentata were administered before irradiation. In conclusion, these results indicate that the extracts of I. dentata have an excellent ability to reduce the radicals and they have a protective effect on DNA breakage caused by radiation.

Keywords

References

  1. Bowden RD, MR Buckwalter, JF McBride, DA Johnson, BK Murray and KL O'Neill. 2003. Tail profile: a more accurate system for analyzing DNA damage using the Comet assay. Mutat. Res. 537:1-9 https://doi.org/10.1016/S1383-5718(03)00056-1
  2. Bramer I, C Herskind, O Haase, HP Rodemann and E Dikomey. 2004. Induction and repair of radiation-induced DNA double-strand breaks in human fibroblasts are not affected by terminal differentiation. DNA Repair. 3:113-120 https://doi.org/10.1016/j.dnarep.2003.10.006
  3. Chung HS, HJ Jeong, MJ Han, ST Park, KK Seong, SH Baek, DM Jeong, MJ Kim and HM Kim. 2002. Nitric oxide and tumor necrosis factor$\alpha$ production by Ixeris dentata in mouse peritoneal macrophages. J. Ethnopharmacology. 82:217-222 https://doi.org/10.1016/S0378-8741(02)00188-5
  4. EI-Demerdash, FM. 2001. Effects of selenium and mercury on the enzymatic activities and lipid peroxidation in brain, liver, and blood of rats. J. Environ. Sci. Health B. 36:489-499 https://doi.org/10.1081/PFC-100104191
  5. Fukuda S, S Tsuchikira and H Iida. 2004. Age-related changes in blood pressure, hematological values, concentrations of serum biochemical constituents and weights of organs in the SHRJIzm, SHRSP/Izm and WKY/Izm. Exp. Anim. 53:67-72 https://doi.org/10.1538/expanim.53.67
  6. Grover P, BS Banu, KD Devi and S Begum. 2001. In vivo genotoxic effects of mercuric chloride in rat peripheral blood leucocytes using comet assay. Toxicology 167: 191-197 https://doi.org/10.1016/S0300-483X(01)00469-3
  7. Hsiao G, MY Shen, KH Lin, MH Lan, LY Wu, DS Chou, CH Lin, CH Su and JR Sheu. 2003. Antioxidative and hepatoprotective effects of Antrodia camphorata extract. J. Agric. Food Chern. 51:3302-3308 https://doi.org/10.1021/jf021159t
  8. Kim JK, CJ Lee, KW Song, BR Do and YD Yoon. 1999. $\gamma$-Radiation accelerates ovarian follicular atresia in immature mice. In Vivo 13:21-24
  9. Kim MK and MS Lee. 1988. Volatile flavor components of Ixeris Dentata and Amaranthus mangostanus. J. Korea Soc. Agri. Chern. Biotech. 31:394-399
  10. Kim MJ, JS Kim, DM Jeong, SS Ham and CY Yu. 2002. Effect of antioxidant, antimutagenicity and anticancer of root extract from Ixeris dentata Nakai. Korean J. Medicinal Crop Sci. 10:222-229
  11. Kim SH. 1995. Inhibitory effects of Ixeris dentata on the mutagenicity of aflatoxin B1, N-methy-N'-nitro-Nnitrosoguanidine and the growth of MG-63 human osteosarcoma cell. J. Korea Soc. Food Sci and Nutr. 24: 304-305
  12. Kumar O, K Sugendran and R Vijayaraghavan. 2003. Oxidative stress associated hepatic and renal toxicity induced by ricin in mice. Toxicon. 41:333-338 https://doi.org/10.1016/S0041-0101(02)00313-6
  13. Lim SS and JH Lee. 1997. Effect of Aster scaber and Ixeris dentata on contractility and vasodilation of cardiovascula and endothelial cell in hyperlidemic rat. J. Korean Soc. Food Sci. Nutr. 26:300-307
  14. Vijayalaxmi RJ, TS Reiter, Herman and ML Meltz. 1998. Melatonin reduces gamma radiation-induced primary DNA damage in human blood lymphocytes. Mutat. Res. 397:203 - 208 https://doi.org/10.1016/S0027-5107(97)00211-X
  15. Vorobtsova I, A Semenov, N Timofeyeva, A Kanayeva and I Zvereva. 2001. An investigation of the age-dependency of chromosome abnormalities in human populations exposed to low-dose ionizing radiation. Mech. Age. Devel. 122:1373-1382 https://doi.org/10.1016/S0047-6374(01)00275-5
  16. Xiong Q, S Kadota, T Tadata and T Namba. 1996. Antioxidative effects of phenylethanoids from Cistanche deserticola. Biol. Pharm. Bull. 19: 1580-1585 https://doi.org/10.1248/bpb.19.1580
  17. Yildiz D, T Akcay, Z Alademir and H Ilkova. 2002 Assessment of DNA base oxidation and glutathione level in patients with type 2 diabetes. Mutat. Res. 505:75-81 https://doi.org/10.1016/S0027-5107(02)00143-4
  18. Sener G, N Jahovic, O Tosun, BM Atasoy and BC Yegen. 2003. Melatonin ameliorates ionizing radiation-induced oxidative organ damage in rat. Life Sci. 74:563-572 https://doi.org/10.1016/j.lfs.2003.05.011