Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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CYTOTOXICITY OF RESIN-BASED ROOT CANAL SEALER, ADSEAL

레진 계열 근관봉함재 Adseal의 세포독성에 관한 연구

  • Kim, Hee-Jung (Department of Conservative Dentistry, College of Dentistry, Seoul National University) ;
  • Baek, Seung-Ho (Department of Conservative Dentistry, College of Dentistry, Seoul National University) ;
  • Lee, Woo-Cheol (Department of Conservative Dentistry, College of Dentistry, Seoul National University) ;
  • Park, Han-Soo (Department of Conservative Dentistry, College of Dentistry, Seoul National University) ;
  • Bae, Kwang-Shik (Department of Conservative Dentistry, College of Dentistry, Seoul National University)
  • 김희정 (서울대학교 치과대학 치과보존학교실) ;
  • 백승호 (서울대학교 치과대학 치과보존학교실) ;
  • 이우철 (서울대학교 치과대학 치과보존학교실) ;
  • 박한수 (서울대학교 치과대학 치과보존학교실) ;
  • 배광식 (서울대학교 치과대학 치과보존학교실)
  • Published : 2004.11.01
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Abstract

The properties of ideal root canal sealers include the ability of sealing the total root canal system and no toxic effects to periradicular tissues. Cytotoxicity test using cell culture is a common screening method for evaluation of the biocompatibility of root canal sealers. The purpose of this study was to investigate the cytotoxic effect of newly developed resin-based sealer (Adseal 1, 2, and 3) comparing with those commercial resin-based sealers (AH26 and AH Plus), ZOE-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT) and calcium hydroxide based sealer (Sealapex), An indirect contact test of cytotoxicity by agar diffusion was performed according to the international standard ISO 10993-5. L929 fibroblast cells were incubated at $37^{\circ}C$ in humidified 5% $CO_2-containing$ air atmosphere. The freshly mixed test materials were inserted into glass rings of internal diameter 5 mm and height 5 mm placed on the agar. After the 24 hrs incubation period, the decolorization zones around the test materials were assessed using an inverted microscope with a calibrated screen. A Decolorization Index was determined for each specimen. Adseal 1. 2, and 3 did not exert any cytotoxic effects, whereas AH26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT, and Sealapex produced mild cytotoxicity.

Keywords

References

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