Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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High Frequency Shoot Regeneration from Leaf Explants of Some Chrysanthemum Cultivars

  • Kim, Young Hoe (Department of Horticulture, Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Park, So Hyeon (Department of Horticulture, Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Kim, Gyeong Hee (Department of Horticulture, Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Jeong, Byoung Ryong (Department of Horticulture, Division of Applied Life Science, Graduate School, Gyeongsang National University)
  • Published : 2004.03.01
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Abstract

This study was conducted to examine differences in shoot regeneration among chrysanthemum cultivars. Leaf explants of chrysanthemum cultivars 'Sulhwa', 'Puma', 'Geummokseo' and 'Sulpoong' were used. Explants cultured on the medium for 2 weeks formed calli at the cut surfaces. Shoots regenerated on MS basal medium supplemented with various concentration combinations of NAA and BAP. Explants were cultured under cool-white fluorescent lamps with a light intensity of $40\mu{Mm}^{-2}$.$s^{-1}$ for 16 $hday^{-1}$, at $25^{\circ}c$ and 70-80% relative humidity. 'Geummokseo' and 'Sulpoong' were the most responsive cultivars in shoot regeneration. Most effective medium for 'Sulhwa' and 'Puma' was MS basal medium supplemented with 10.0 $\mu{M}$ NAA and 5.0 $\mu{M}$ BAP and for 'Geummokseo' MS supplemented with 10.0$\mu{M}$ NAA and 20.0$\mu{M}$ BAP. Regeneration of multiple shoots was observed on MS basal medium supplemented with 1.0$\mu{M}$ or 10.0 $\mu{M}$ NAA and 5.0$\mu{M}$ BAP. High frequency regeneration of adventitious shoots from leaf explants and efficient induction of root from these regenerated shoots were obtained.

Keywords

References

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