Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
권호 표지
DOI QR코드

DOI QR Code

ANTIBIOTIC SUSCEPTIBILITY IN MUTANS STREPTOCOCCI AND STREPTOCOCCUS ANGINOSUS ISOLATED FROM DENTAL PLAQUE

치면세균막에서 분리한 뮤탄스 연쇄상구균 및 Streptococcus anginosus의 수종 항생제에 대한 감수성 조사

  • Kook, Joong-Ki (Department of Oral Biochemistry, Oral Biology Research Institute, College of Dentistry, Chosun University) ;
  • Lim, Sang-Soo (Department of Conservative Dentistry, Oral Biology Research Institute, College of Dentistry, Chosun University) ;
  • Yoo, So-Young (Department of Oral Biochemistry, Oral Biology Research Institute, College of Dentistry, Chosun University) ;
  • Hwang, Ho-Keel (Department of Conservative Dentistry, Oral Biology Research Institute, College of Dentistry, Chosun University)
  • 국중기 (조선대학교 치과대학 구강생화학교실, 구강생물연구소) ;
  • 임상수 (조선대학교 치과대학 보존학교실, 구강생물연구소) ;
  • 유소영 (조선대학교 치과대학 구강생화학교실, 구강생물연구소) ;
  • 황호길 (조선대학교 치과대학 보존학교실, 구강생물연구소)
  • Published : 2004.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to investigate the susceptibility of mutans streptococci (S. mutans and S. sobrinus) and Streptococcus anginosus, for seven antibiotics, penicillin G, amoxicillin, ciprofloxacin, cefuroxime, erythromycin, bacitracin, and vancomycin. The minimum inhibitory concentration (MIC) of seven antibiotics against 3 species (type strains) of mutans streptococci and S. anginosus, 10 strains (wild type) of S. mutans, 7 strains (wild type) of S. sobrinus, and 11 strains (wild type) of S. anginosus, were measured by broth dilution method. All of the type strains of mutans streptococci and S. anginosus had the same susceptibility for penicillin G, amoxicillin, cefuroxime and bacitracin. Type strain of S. anginosus was sensitive in ciprofloxacin, but those of mutans streptococci were not. All of the clinical isolates of mutans streptococci and S. anginosus had the same susceptibility for the seven antibiotics. Our data reveal that mutans streptococci and S. anginosus have similar antibiotic-resistant character. In addition. these results may offer the basic data to verify the antibiotic-resistant mechanism of mutans streptococci and S. anginosus.

Keywords

References

  1. Ruoff KL, Whiley RA, Beighton D. Streptococcus. In: Murray ER, Baron EJ, Pfaller MA, Tenover FC and Yolken RH (Eds.), Manual of CLINICAL MICROBIOLOGY. 7th Ed. ASM press. Washington, p283-296, 1999
  2. Whiley RA, Beighton D. Current classification of the oral streptococci. Oral Microbiol Immunol 13:195-216, 1998 https://doi.org/10.1111/j.1399-302X.1998.tb00698.x
  3. Igarashi T, Ichikawa K, Yamamoto A, Goto N. Identification of mutans streptococcal species by the PCR products of the dex genes. J Microbiol Methods 46:99-105, 2001 https://doi.org/10.1016/S0167-7012(01)00263-9
  4. Gold OG, Jordan HV, van Houte J. A selective medium for the isolation of Streptococcus mutans. Arch Oral Biol 18:1357-1364, 1973 https://doi.org/10.1016/0003-9969(73)90109-X
  5. Little WA, Korts DC, Thomson LA, Bowen WH. Comparative recovery of Streptococcus mutans on ten isolation media. J Clin Microbiol 5(6):578-583, 1977
  6. Tanzer JM, Borjesson AC, Laskowski L, Kurasz AB, Testa M. Glucose-sucrose-potassium tellurite-bacitracin agar, an alternative to mitis salivarius- bacitracin agar for enumeration of Streptococcus mutans. J Clin Microbiol 20(4):653-659, 1984
  7. Kim PS, Hwang HK, Kim H-S, Lim S-A, Kang HY, Yoo SY, Kook J-K. Identification of non-mutans btreptococci growing on Mitis-Salivarius Bacitracin agar medium. J Dent Res 82(Special issue B):B-351, 2003
  8. Shklair IL, Keene HJ. A biochemical scheme for the separation of the five varieties of Streptococcus mutans. Archs Oral Biol 19:1079-1081, 1974 https://doi.org/10.1016/0003-9969(74)90099-5
  9. Shklair IL, Keene HJ. Biochemical characterization and distribution of Streptococcus mutans in three diverse populations, In: Stiles HM, Loesche WJ, O' Brien TC (Eds,), Microbial aspects of dental caries. Information Retrieval Inc., Washington, D.D. p201-210, 1976
  10. Murray PR, Jorgensen JH. Quantitative susceptibility test methods in major united states medical center Antimicrob Agents Chemother 20(1):66-70, 1981 https://doi.org/10.1128/AAC.20.1.66
  11. Jorgensen JH, Turnidge JD, Washington A. Antibacterial susceptibility tests: dilution and disk diffusion methods, In: Murray ER, Baron EJ, pfaller MA, Tenover FC, Yolken RH (Eds.), Manual of CLINICAL MICROBIOLOGY. 7th Ed. ASM press. Washington, p1526-1543, 1999
  12. Bancescu G, Skaug N, Dumitriu S, Bancescu A, Antimicrobial susceptibility of some streptococci strains of anginosus group isolated from oral and maxillofacial infections. Roum Arch Microbiol Immunol 58(1):57-63, 1999
  13. Horii T, Arakawa Y, Ohta M, Ichiyama S, Wacharotayankun R, Kato N. Plasmid-mediated AmpC-type beta-lactamase isolated from Klebsiella pneumoniae confers resistance to broad-spectrum beta-lactams, including moxalactam. Antimicrob Agents Chemother 37(5):984-990, 1993 https://doi.org/10.1128/AAC.37.5.984
  14. Cloeckaert A, Baucheron S, Flaujac G, Schwarz S, Kehrenberg C, Martel JL, Chaslus-Dancla E. Plasmid-mediated florfenicol resistance encoded by the floR gene in Escherichia coli isolated from cattle. Antimicrob Agents Chemother 44(10):2858-2860, 2000 https://doi.org/10.1128/AAC.44.10.2858-2860.2000
  15. Simjee S, White DG, McDermott PF, Wagner DD, Zervos MJ, Donabedian SM, English LL, Hayes JR, Walker RD. Characterization of Tn 1546 in van-comycin- resistant Enterococcus faecium isolated from canine urinary tract infections: evidence of gene exchange between human and animal enterococci. J Clin Microbiol 40(12):4659-4665, 2002 https://doi.org/10.1128/JCM.40.12.4659-4665.2002
  16. Bauernfeind A, Wagner S, Jungwirth R, Schneider I, Meyer D. A novel class C beta-lactamase(FOX-2) in Escherichia coli conferring resistance to cephamycins. Antimicrob Agents Chemother 41(9):2041-2046, 1997
  17. Nelson EC, Elisha BG. Molecular basis of AmpC hyperproduction in clinical isolates of Escherichia coli. Antimicrob Agents Chemother 43(4):957-959, 1999
  18. Bonnet R, Chanal C, Ageron E, Sirot D, De Champs C, Grimont P, Sirot J. Inducible AmpC beta-lactamase of a new member Enterobacteriaceae. Antimicrob Agents Chemother 46(10):3316-3319, 2002 https://doi.org/10.1128/AAC.46.10.3316-3319.2002
  19. Petrosino JF, Pendleton AR, Weiner JH, Rosenberg SM. Chromosomal system for studying AmpC-mediated beta-lactam resistance mutation in Escherichia coli. Antimicrob Agents Chemother 46(5):1535-1539, 2002 https://doi.org/10.1128/AAC.46.5.1535-1539.2002
  20. Noguchi N, Emura A, Matsuyama H, O' Hara K, Sasatsu M, Kono M. Nucleotide sequence and characterization of erythromycin resistance determinant that encodes macrolide 2' -phosphotransferase I in Escherichia coli. Antimicrob Agents Chemother 39(10):2359-2363, 1995 https://doi.org/10.1128/AAC.39.10.2359
  21. van Boxtel RA, van de Klundert JA. Expression of the Pseudomonas aeruginosa gentamicin resistance gene aacC3 in Escherichia coli. Antimicrob Agents Chemother 42(12):3173-3178, 1998
  22. Gotoh N, Tsujimoto H, Poole K, Yamagishi J, Nishino T. The outer membrane protein OprM of Pseudomonas aeruginosa is encoded by oprK of the mexA-mexB-oprK multidrug resistance operon. Antimicrob Agents Chemother 39(11):2567-2569, 1995 https://doi.org/10.1128/AAC.39.11.2567
  23. Mitchell BA, Brown MH, Skurray RA. QacA multidrug efflux pump from Staphylococcus aureus: comparative analysis of resistance to diamidines, biguanidines, and guanylhydrazones. Antimicrob Agents Chemother 42(2):475-477, 1998 https://doi.org/10.1093/jac/42.4.475
  24. Kaatz GW, Seo SM, O' Brien L, Wahiduzzaman M, Foster TJ. Evidence for the existence of a multidrug efflux transporter distinct from NorA in Staphylococcus aureus. Antimicrob Agents Chemother 44(5):1404-1406, 2000 https://doi.org/10.1128/AAC.44.5.1404-1406.2000
  25. Siqueira JF, Rocas IN, Moraes SR, Santos KR. Direct amplification of rRNA gene sequences for identification of selected oral pathogens in root canal infections. Int Endod J 35(4):345-351, 2002 https://doi.org/10.1046/j.1365-2591.2002.00485.x
  26. Tateda M, Shiga K, Saijo S, Sone M, Hori T, Yokoyama J, Matsuura K, Takasaka T, Miyagi T. Streptococcus anginosus in head and neck squamous cell carcinoma: implication in carcinogenesis. Int J Mol Med 6(6):699-703, 2000
  27. Shiga K, Tateda M, Saijo S, Hori T, Sato I, Tateno H, Matsuura K, Takasaka T, Miyagi T. Presence of Streptococcus infection in extra-oropharyngeal head and neck squamous cell carcinoma and its implication in carcinogenesis. Oncol Rep 8(2) :245-248, 2001
  28. 김미성. 김수관. 정해만. 김생곤. 국중기. 김미광. 김화숙. 유소영. 분자생물학적 기법을 이용한 악골골수염 병소의 세균 동정. 대한구강악안면외과학회 29(1):48-55, 2003