International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Molecular Cloning of a LIM Protein cDNA from the Mulberry Longicorn Beetle, Apriona germari

  • Gui, Zhongzheng (College of Natural Resources and Life Science, Dong-A University) ;
  • Wei, Yadong (College of Natural Resources and Life Science, Dong-A University) ;
  • Yoon, Hyung Joo (Department of Agricultural Biology, National Institute of Agricultural science & Technology) ;
  • Kim, Iksoo (Department of Agricultural Biology, National Institute of Agricultural science & Technology) ;
  • Guo, Xijie (Sericultural Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Jin, Byung Rae (College of Natural Resources and Life Science, Dong-A University) ;
  • Sohn, Hung Dae (College of Natural Resources and Life Science, Dong-A University)
  • Published : 2004.09.01
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Abstract

Here we report the molecular cloning of a LIM protein cDNA of the CRP (cysteine-rich protein) family from the mulberry longicorn beetle, Apriona, geramri. The A. germari LIM protein cDNA contains an open reading frame of 276 bp encoding 92 amino acid residues with a calculated molecular weight of approximately 10 kDa. The A. germari LIM protein contains the cysteine-rich consensus sequence of LIM domain and the glycine-rich consensus sequence observed in cysteine-rich protein family 1 (CRP1). The potential nuclear targeting signal is retained. The deduced amino acid sequence of the A. germari LIM protein cDNA showed 81 % identity to both Bombyx mori muscle LIM protein (Mlp) and Drosophila melanogaster Mlp60A and 77% to Epiblema scudderiana Mlp. Northern blot analysis showed that A. germari LIM protein is highly expressed in epidermis and muscle, and less strongly in midgut, but not in the fat body.

Keywords

References

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