Journal of Plant Biotechnology

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

Shoot Organogenesis and Plantlet Regeneration from Stem Explants of Cleome rosea Vahl (Capparaceae)

  • Claudia Simoes (Departamento de Biologia Vegetal/Laboratorio de Biotecnologia de Plantas (LABPLAN) - Instituto de Biologia Roberto Alcantara Gomes) ;
  • Alessandra S. Santos (Universidade do Estado do Rio de Janeiro (UERJ)) ;
  • Norma Albarello (Universidade do Estado do Rio de Janeiro (UERJ)) ;
  • Solange Faria Lua Figueiredo (Universidade do Estado do Rio de Janeiro (UERJ))
  • 발행 : 2004.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The medicinal value of the genus Cleome justifies bio-technological studies of Cleome rosea, a Brazilian annual species from sandy coastal ecosystems (restinga), which have been submitted to an intense process of antropogenic degradation. In the present work, was analyzed the influence of cytokinins, 6-benzyladenine (BA) and 6-furfurylaminopurine (kinetin) added to the Murashige and Skoog medium (MS), on the proliferation capacity of explants from the stem axis (hypocotyl, node and internode) for a period of five monthly subcultures (150 days). Regardless of the explant sources, plantlet regeneration by direct and indirect organogenesis was observed. The largest number of shoots proliferated through direct organogenesis was obtained on medium with 4.4 $\mu{M}$ BA. Also, the highest proliferation capacity through indirect organogenesis was found on medium with 4.4 $\mu{M}$ BA + 4.6 $\mu{M}$ kinetin. The presence of kinetin alone was not effective for multiplication of the species. Elongation and rooting were obtained when shoots were transferred onto growth regulator-free medium, and acclimatization rates from 70% to 81% were achieved depending on explant sources used. Plants were then successfully established in soil and showed normal phenotypes.

키워드

참고문헌

  1. Ahmed ZF, Riszk AM, Hammouda FM, Seif EI-nasr MM (1972) Naturally occuring glucosinolates with special reference to those of family Capparidaceae. Planta Med 21: 35-60
  2. Borthakur M, Hazarika J, Singh RS (1998) A protocol for micropropagation of Alpinia galanga. Plant Cell Tiss Org Cult 55: 231-233
  3. Brassard N, Brissette L, Lord D, Laliberte S (1996) Elongation, rooting and acclimatization of micropropagated shoots from mature material of hybrid larch. Plant Cell Tiss Org Cult 44: 37-44
  4. Brummitt RK (1992) Vascular plants families and genera. Royal Botanic Gardens, Kew
  5. ChaIker-Scoot L (1999) Environmental significance of anthocyanins in plant stress responses. Photochem Photobiol 70: 1-9
  6. Deora NS, Shekhawat NS (1995) Micropropagation of Capparis decidua (Forsk.) Edgew. - a tree of arid horticulture. Plant Cell Rep 15: 278-281
  7. Di Stasi LC, Hiruma CA, Guimaraes EM, Santos CM (1994) Medicinal plants popularly used in Brazilian Amazon Fitoterapia LXV (6): 529-540
  8. Fransa SC, Bertoni BW, Pereira MAS (1995) Antihepatotoxic agent in micropropagated plantlets of Eclipta alba. Plant Cell Tiss Org Cult 40: 295-299
  9. Forsyth C, Van Staden J (1987) Cytokinin metabolism in tomato plants. II. Metabolites of kinetin and benzyladenine in decapited roots. Plant Growth Reg 6: 277-292
  10. Grattapaglia D, Machado MA (1998) Micropropagasao. In: Torres AC, Caldas LS, Buso JA (eds), Cultura de Tecidos e Transformasao Genetica de Plantas Vol 1. pp 183-260. SPI, Brasilia
  11. Geneve RL (1990) Fasciation with fascination. Amer Horticult 68: 26-31
  12. Hashem FA, Wahba HE (2000) Isothiocyanates in myrosinase treated herb extract of Cleome chrysantha Decne. and their antimicrobial activities. Phytotherapy Res 14: 284-287
  13. Hopkins WG (1995) Introduction to Plant Physiology. $1^{st}$ edn.John Wiley & Sons Inc, New York
  14. Hu CY, Wang PJ (1983) Meristem, shoot tip and bud cultures. In: Evans DA, Sharp WR, Ammirato PV, Yamada Y. (eds), Handbook of Plant Cell Culture. Vol 1. pp 177-227 Macmillan, New York
  15. Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss Org Cult 33: 105-119
  16. Hussey G (1986) In vitro propagation of horticultural and agricultural crops, In: Mantell SH, Smith H. (eds). Plant Biotechnology, pp 111-137. Cambridge University Press, Cambridge
  17. Murashige T, Skoog FA (1962) Revised medium for rapid growth and biossays with tobacco tissue cultures. Physiol Plant 15: 473-497
  18. Nagaya H, Tobita Y, Nagae T, Itokawa H, Takeya K, Halim AF, AbdeI-HaIim O (1997) Cytotoxic triterpenes from Cleome afncana. Phytochemistry 44: 1115-1119
  19. Nassem M, Jha KK (1994) Differentiation and regeneration in Cteome leaves culture in vitro. Egypt J Bot 1: 37-49
  20. Nassem M, Jha KK (1997) Rapid clonal multiplication of Cleome gynandra DC, through tissue culture. Phytomorphology 47: 405-411
  21. Nicola WG, Ibrahim KM, Mikhail TH, Girgis RB, Khadre ME (1996) Role of the hypoglycemic plant extract Cleome drosehfolia in improving glucose and lipid metabolism and its relation to insulin resistance in fatty liver. Boll Chim Farm 135: 507-517
  22. Nieuwkerk JP, Zimmerman RH, Fordham I (1986) Thidiazuron stimulation of apple shoot proliferation in vitro. Hortscience 21: 516-518
  23. Preece JE, Huetteman CA, Ashby WC, Roth PL (1991) Micro and cutting propagation of silver maple. I. Results with adults and juvenile propagules. J Amer Soc Hort Sci 116: 142-148
  24. Rodriguez R, Rey M, Cuozzo L, Ancora G (1990) In vitro propagation of caper (Cappahs spinosa L). In vitro Cell Dev Biol 26: 531-536
  25. Safrazbekyan SA, Kataeva NV, Milyaeva EL (1990) Morpholo-gical properties of caper (Cappahs spinosa L.) shoots during clonal micropropagation. Fiziol Rast 37: 169-176
  26. Selloum L, Arrar L, Medani B, Khenchouche A, Bisker H (1995) Effect of Cleome arabica leaves extract on inflammatory cells response in rat. Biochem Soc T 23: 609
  27. Selloum L, Sebihi L, Mekhalfia A, Mahdadi R, Senator A (1997) Antioxidant activity of Cleome arabica leaves extract. Biochem Soc T 25: S608
  28. Sharma V, Padhya MA (1996) In vitro rapid multiplication and propagation of Crataeva nurvala, Indian J Exp Biol 34: 243-246
  29. Shvastava PS, Glock H (1987) FPA-induced fasciation of shoots of birch in vitro. Phytomorphology 37: 395-399
  30. Tyagi P, Kothari SL (2001) Continuous shoot production for micropropagation of Capparis decidua - A tree of arid agroforestry system. J Indian Bot Soc 80: 5-8
  31. Yusnita S, Geneve RL, Kester ST (1990) Micropropagation of white flowering eastern redbud (Cercis canadensis var. alba L). J Environ Hort 8: 177-179