Direct Organogenesis in Geophila reniformis D. Don., an Important Medicinal Herb

  • Nisha, A. (Food Microbiology Department, Central Food Technological Research Institute) ;
  • Narasimhan, S. (Department of Botany, University of Kerala, Thiruvananthapuram) ;
  • Manjula, S. (Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram) ;
  • Nair, G.M. (Tropical Botanic Garden & Research Institute, Palode, Thiruvananthapuram)
  • 발행 : 2004.09.01

초록

Adventitious multiple shoots were developed from leaf, petiole and internode explants of Geophila reniformis D. Don. on MS medium supplemented with various concentrations of $N^6$-benzylaminopurine (BAP) or Kinetin (KIN) alone or in combination with indole-3-acetic acid (IAA). Leaf showed maximum organogenetic potential, followed by petiole and internode. Murashige and Skoog (MS) medium supplemented with 22.22 $\mu{M}$ BAP and 4.57 $\mu{M}$ IAA induced maximum shoot buds from leaf explants. Internodal segments showed low potential of direct organogenesis. The regenerated shoots rooted the best in presence of 10.75 - 13.44 $\mu{M}$ $\alpha$-naphthalene acetic acid (NAA) along with 2.22 $\mu{M}$ BAP, and were successfully established in the field with a survival rate of 89.11%.

키워드

참고문헌

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