Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antitumor activity of Bacillus subtilis SW-1 isolated from Jeotgal

젓갈에서 분리한 Bacillus subtilis SW-1의 항암활성

  • 박종기 (진주산업대학교 미생물공학과) ;
  • 조용운 (진주산업대학교 미생물공학과) ;
  • 최영우 (신라대학교 식품영양학과) ;
  • 정영기 (동의대학교 미생물학과) ;
  • 갈상완 (진주산업대학교 미생물공학과)
  • Published : 2004.10.01
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Abstract

A bacterum containing antitumor activity was isolated from traditional korean food, Jeotgal. Through the 16s rRNA sequence analysis, the bacterium was identitied as a strain of Bacillus subtilis SW-l. The best culture condition for antitumor activity of the bacterium is 3% of soluble starch and 1 % of yeast extract as corbon and nitrogen sources, respectively. Cytotoxicitic concentrations of the culture supernatant of B. subtilis SW-1 against cancer cell lines, A549 and SK-OV3 were 30 ul/ml and 40 ul/ml, respectively, as $IC_{50}$/ values. In DNA fragmentation assay, the culture supernatant showed the programmed cell death (apoptosis) to cause degrading the chromosomal DNA like ladder. Taken together, the culture supernatant of the B. subtilis SW-1 has some possibility to be used as an antitumor agent.

본 연구에서 미생물유래의 종양 예방 및 억제제 개발을 목적으로 우리나라 전통 발효식품인 젓갈에서 항암 활성이 우수한 미생물을 분리, 동정 하였다. 젓갈로부터 항암물질을 생산하는 균주 SW-1을 분리하여 형태학적, 생리학적 특성을 검토한 결과, 본 균주는 Bacillus subtillis SW-1으로 동정되었다. 본 균주로부터 항암물질은 실험에 사용한 두가지의 암세포에 강한 활성을 나타내었다. 그 중에서 폐암 세포인 A549에 가장 강한 활성을 나타내었다. 항암물질 생산을 위한 최적 배양조건을 검토한 결과, 3.0% soluble starch, 1.0% yeast extract 였다. 무기염의 경우는 활성에는 그다지 영향을 미치지 않았다. 배지의 초기 pH 및 배양온도에 따른 항암물질의 생산성을 검토한 결과 초기 pH 5.0배양온도 3$0^{\circ}C$에서 25시 간배양 했을 때 최대 활성을 보였다. Chromosomal DNA단편화 실험에서 Bacillus subtilis SW-1의 배양상등액을 처리했을 때 DNA가 사닥다리모양으로 분해되는 Apoptosis(계획된 세포죽임)를 일으키는 것으로 확인되었다. 이 미생물이 생성하는 물질을 현재 HPLC, GC등을 이용하여 분리 중에 있으며 현재까지의 결과로는 당 중합체 인 것으로 분석된다.

Keywords

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