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Effect of microbial product made of Bacillus stearothermophilus DL-3 on microorganisms in soil and growth of lettuce and Chinese cabbage.

Bacillus stearothermophilus DL-3 미생물 제재의 처리가 토양 미생물상 및 상추와 배추의 생장에 미치는 영향

  • 김순희 (동아대학교 생명자원과하대학 응용생물학과) ;
  • 배계선 (동아대학교 생명자원과하대학 응용생물학과) ;
  • 양재균 (동아대학교 생명자원과학대학 생명공학과) ;
  • 이유정 (동아대학교 생명자원과학대학 생명공학과) ;
  • 오주성 (동아대학교 생명자원과하대학 원예식물생명공학과) ;
  • 정순재 (동아대학교 생명자원과학대학 원예식물생명공학과) ;
  • 문병주 (동아대학교 생명자원과학대학 응용생물학과) ;
  • 이진우 (동아대학교 생명자원과학대학 생명공학과)
  • Published : 2004.10.01

Abstract

Effect of the microbial product made of Bacillus stearothermophilus DL-3, which was isolated from the soil and identified in this study, and rice bran on microorganisms in soil and growth of lettuce (Red skirt lettuce) and Chinese cabbage (Ga rack new No.1 Chinese cabbage) was investigated. Total numbers of microorganisms in the pot with untreated soil, treated with standard amount of microbial product and treated with double amounts of microbial product for growth of lettuce after 6 weeks were 2.78${\times}$10$^{7}$ CFU/g, 2.72${\times}$10$^{8}$ CFU/g and 3.63${\times}$10$^{8}$ CFU/g. Total numbers of microorganisms in the soil without treatment of microbial product and treated with standard amount of microbial product were 2.06${\times}$10$^{8}$ CFU/g and 5.49${\times}$10$^{8}$ CFU/g. Total numbers of microorganisms in the pot with untreated soil, treated with standard amount of microbial product and treated with double amounts of microbial product for growth of Chinse cabbage after 6 weeks were 1.43${\times}$10$^{7}$ CFU/g, 3.42${\times}$10$^{8}$ CFU/g and 7.22${\times}$10$^{8}$ CFU/g. Total numbers of microorganisms in the soil without treatment of microbial product and treated with standard amount of microbial product were 5.75${\times}$10$^{8}$ CFU/g and 7.96${\times}$10$^{8}$ CFU/g. On basis of leaf length, leaf width, leaf number, wet weight and dry weight, the growth of lettuce and Chinese cabbage on the soil treated with microbial product was faster than that on the untreated soil. The treatment of microbial product in the soil resulted in the increase of useful microorganisms, which seemed to enhance the growth of lettuce and Chinese cabbage.

유기물이 풍부한 토양에서 분리하여 동정한 Bacillus stearothermophilus DL-3와 미강을 사용하여 미생물 제재를 제조하고 미생물 제재의 처리가 상추(적치마 상추)와 배추(가락 신1호 배추)재배 토양의 미생물상에 미치는 영향 및 상추와 배추의 생장에 미치는 영향을 조사하였다. 미생물 제재를 처리 한 6주 후, 미생물 제재를 처 리 한 토양에 존재하는 토양 미생물의 총 균수는 미생물 제재를 처리하지 않은 토양에 존재하는 토양 미생물의 총 균수에 비하여 미생물 제재를 처리한 양에 비례하여 많음을 확인하였다. 미생물 제재를 처리한 토양에서 재배한 상추와 배추의 생육이 미생물 제재를 처리하지 않은 토양에서 재배한 상추와 배추에 비하여 빠름을 확인하였다. 미생물 제재를 처리한 토양에서는 처리하지 않은 토양에 비하여 전체적인 토양 미생물 수의 증가와 작물의 생장에 유용한 종류의 미생물이 상대적으로 증가하기 때문에 상추와 배추의 생장을 촉진시킨다고 판단된다.

Keywords

References

  1. Alexander, M. 1982. Most probable number method for microbial populations, pp. 815-820, In Page, A. L, R. H. Miller and D. R. Keeney (eds.), In methods of soil analysis, Part 2. Chemical and microbiological properties. American society of agronomy and soil science society of America, Inc., Madison, Wisconsin
  2. Carmelo, V., A. Florido, I. Vinhas and J. C. Roseiro. 2002. Physiological response of Bacillus stearothermophilus continuous culture to carbon source concentration and temperature shifts. Proc. Biochem. 38, 763- 770 https://doi.org/10.1016/S0032-9592(02)00222-4
  3. Ehab, R. E. and M. E. Amany. 1999. Lichenase production by catabolite repression-resistant Bacillus subtilis mutants: optimization of an agro- industrial by-product medium. Enz. Microb. Technol. 24, 325-331 https://doi.org/10.1016/S0141-0229(98)00125-2
  4. Elad, Y., I. Chet and Y. Henis. 1982. Degradation of plant pathogenic fungi by Tricoderma harzianum. Can. J. Microbiol. 28, 719-725 https://doi.org/10.1139/m82-110
  5. Ferrero, M. A., G. R. Castro, C. M. Abate, M. D. Baigori and F. Sineriz. 1996. Thermostable alkaline proteases of Bacillus licheniformis MIR 29; isolation production and characterization. Appl. Microbiol. Biotechnol. 45, 327-332 https://doi.org/10.1007/s002530050691
  6. Jung, W. S., Y. H. Kim and C. J. Go. 1999. Microbiology fermentation biotechnology: collection of soil Actinomycetes from Cheju island and screening for their antibacterial activities. Kor. Soc. Agric. Chem. Bietechnol. 42, 99-104
  7. Kim, J. M., C. W. Son, H. P. Seo, B. J. Moon, S. K. Do and J. W. Lee. 2002. Effect of microbial product on microorganisms in soil and growth of cabbage. Kor. J. Life Sci. 12, 515-522
  8. Kim, K. J. and S. K. Kim. 1998. Effect of soil microbial fertilizers on yield of Chinese cabbage (Brassica campestris L.). Kor. J. Hort. Sci. Tech. 16, 341-343
  9. Kim, S. J. and S. K. Shin. 1997. Effects of bioremediation products on the oil degradability. Kor. J. Microbiol. 33, 157-162.
  10. Lee, H. S., S. G. Cho and Y. J. Choi. 1996. Pentose utilization by the xylanolytic bacterium Bacillus stearothermophilus. Kor. J. Appl. Microbiol. Biotechnol. 24, 385-92
  11. Lee, K. B., Y. W. Kim and K. S. Kim. 1988. Effects of pesticides on microorganisms related to the nitrogen cycle in the submerged soil. Kor. J. Soil Fer. 21, 149-159
  12. Lee, S. H., S. J. Jung, K. Y. Kim, T. H. Kim and W. S. Kim. 2002. Effect of compost by chitinolytic bacteria chemical and microbial property in pear orchard soil. Kor. J. Hort. Sci. Technol. 20, 100
  13. Park, K. W., J. Y. Chung and S. H. Lee. 2002. Effect of various microbial treatment of several laviatae family herbs germination. Kor. J. Hort. Sci. Technol. 20, 49
  14. Park, S. K. and S. H. Baek. 2000. Effect of yeast extracts concentration on cell growth and products of Bacillus stearothermophilus. Kor. J. Sci. & Nat. Res. 22, 41-54
  15. Raghukumar, C., V. Vipparty, J. J. David and D. Chandramohan. 2001. Degradation of crude oil by marine cyanobacteria. Appl. Microbiol. Biotechnol. 57, 433-436 https://doi.org/10.1007/s002530100784
  16. Simoes, D. C., M. D. McNeil, B. Kristiansen and M. Mattey. 1997. Purification and partial characterization of a 1.57 kDa thermostable esterase from Bacillus stearothermophilus. FEMS Microbiol. Lett. 147, 151-156 https://doi.org/10.1111/j.1574-6968.1997.tb10235.x
  17. Song, H. S. and Y. J. Choi. 1989. Production of xylanase by Bacillus stearothemophilus. Kor. J. Appl. Microbial. Bioeng. 17, 289-294
  18. Sookkheo, B., S. Sinchaikul, S. Phutrakul and S-T. Chen. 2000. Purification and characterization of the highly thermostable proteases from Bacillus stearothermophilus TLS33. Protein Exp. Puri. 20, 142-151 https://doi.org/10.1006/prep.2000.1282
  19. Suh, J. S. and J. S. Shin. Soil microbial diversity of paddy fields in Korea. Kor. J. Soil. Fert. 30, 200-207
  20. Trewavas, A. 2001. Urban myths of organic farming. Nature. 410, 409-410 https://doi.org/10.1038/35068639
  21. Wiseman A. 1975. Handbook of enzyme biotechnology. Wiley, New York
  22. Wollum, A. G. 1982. Cultural methods for soil microorganisms, pp781-802, In Page, A. L, R. H. Miller and D. R. Keeney (eds.), In methods of soil analysis, Part 2. Chemical and microbiological properties. American Society of Agronomy and Soil Science Society of America, Inc., Madison, Wisconsin
  23. Yamada, Y., H. Uemura, H. Nakaya, K. Sakata, T. Takatori, M. Nagao, H. Iwase and K. Iwadate. 1996. Production of hydroxy fatty acid (10-Hydroxy-12(Z)-octadecenoic acid) by Lactobacillus plantarum from linoleic acid and its cardiac effects to guinea pig papillary muscles. Biochem. Biophys. Res. Comm. 226, 391-395 https://doi.org/10.1006/bbrc.1996.1366
  24. Yun, H. J., S. J. Kim and K. H. Min. 1993. Crude oil- degrading properties of psychrotrophic bacterium Acinetobacter calcoaceticus A1-1. Kor. J. Appl. Microbiol. Biotechnol. 21, 74-81
  25. Yun, S. Y and J. D. Shin, 2001. Effects of TLB microbial fertilizer application on soil chemical properties, microbial flora and growth of Chinese cabbage. Kor. J. Soil Sci. Fert. 34, 8-16

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