Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Photoswitching Characteristics of Biodevice Consisting of Chlorophyll $\alpha$ Langmuir-Blodgett Film

  • Nam, Yun-Suk (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Choi, Jeong-Woo (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Lee, Won-Hong (Department of Chemical and Biomolecular Engineering, Sogang University)
  • Published : 2004.10.01
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Abstract

The photoelectric responses of a biodevice consisting of chlorophyll $\alpha$ Langmuir-Blodgett film were investigated. Chlorophyll $\alpha$ Langmuir-Blodgett films were deposited onto ITO and Au coated glass. To confirm film formation, surface analysis of chlorophyll $\alpha$ Langmuir-Blodgett film was carried out by measurement using atomic force microscopy. The metal/insulator/metal structured biodevice was constructed by depositing aluminum onto the chlorophyll $\alpha$ Langmuir-Blodgett film surface. To investigate the photoelectric response, the current-voltage characteristic was measured by the conducting metal tip. The photoswitching function and transient photovoltage characteristics of the proposed device were measured by irradiation with Ar ion laser and $N_2$ pulse laser, respectively. This research suggested that the proposed biodevice consisting of chlorophyll $\alpha$ could be applied to the molecular scale biosensor and/or bioelectronic device.

Keywords

References

  1. Birge, R. R. 1990. Photophysics and molecular electronic applications of the rhodopsin. Annu. Rev. Phys. Chem. 41: 683-733. https://doi.org/10.1146/annurev.pc.41.100190.003343
  2. Choi, H. G., B. K. Oh, W. H. Lee, and J. W. Choi. 2001. Deposition behavior and photoelectrochemical characteristics of chlorophyll a Langmuir-Blodgett films. Biotechnol. Bioprocess Eng. 6: 183- 188.
  3. Choi, J. W., W. Lee, J. M. Cho, Y. K. Kim, S. Y. Park, and W. H. Lee. 2002. Control of fed rate using neurocontroller incorporated with genetic algorithm in fed batch cultivation of Scutellaria baicalensis Georgi. J. Microbiol. Biotechnol. 12: 687- 691.
  4. Choi, J. W., Y. S. Nam, and M. Fujihira 2004. Nanoscale fabrication of biomolecular layer and its application to biodevices. Biotechnol. Bioprocess Eng. 9: 76- 85.
  5. Iida, K., A. Kashiwada, and M. Nango. 2000. Construction of Langmuir-Blodgett films from light-harvesting complex I from photosynthetic: bacteria. Colloids Surf. A 169: 199-208.
  6. Iida, K., A. Kashiwada, M. Mimuro, and M. Nango. 2000. Characterization of the light harvesting polypeptide/ bacteriochlorophyll a complex isolated from photosynthetic bacteria by the linear dichroism spectra. Bull. Chem. Soc. Jpn. 73: 221- 229.
  7. Inyama, K. 1979. Methods of preparing chlorophyll a multilayers on glass plates. Photochem. Photobiol. 29: 633-636.
  8. Iriyama, K., M. Yoshiura, and F. Mizutani. 1980. Deposition of chlorophyll a Langmuir-Blodgett films onto an $SnO_2$ optically transparent electrode. Thin Solid Films 68: 47- 54.
  9. Miyasaka, T., T. Watanabe, A. Fujishima, and K. Honda. 1978. Light energy conversion with chlorophyll monolayer electrode. In vitro electrochemicalsimulation of photosynthetic primary process. J. Am. Chem. Soc. 78: 6657- 6665.
  10. Miyasaka, T., T. Watanabe, A. Fujishima, and K. Honda. 1979. Highly efficient quantum conversion at chlorophyll a lecithin mixed monolayer coated electrodes. Nature 277: 638- 640.
  11. Oh, B. K., Y. K. Kim, Y. M. Bae, W. H. Lee, and J. W. Choi. 2002. Detection of Escherichia coli O157:H7 using immunosensor based on surface plasmon resonance. J. Microbiol. Biotechnol. 12: 780- 786.
  12. Tkachenko, N. V.. P. H. Hynninen, and H. Lemmetyinen. 1996. Photoelectric signals of chlorophyll a LangmuirBlodgett films. Chem. Phys. Lett. 261: 234- 240.