Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Polyvinyl Alcohol Degradation by Microbacterium barkeri KCCM 10507 and Paenibacillus amylolyticus KCCM 10508 in Dyeing Wastewater

  • Published : 2004.10.01
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Abstract

The purpose of this study was to investigate the degradation of PVA (polyvinyl alcohol) contained in dyeing wastewater by a mixed culture of Microbacterium barkeri KCCM 10507 and Paenibacillus amylolyticus KCCM 10508. Firstly, synthetic wastewater which contained different initial concentrations of PVA varying from 50 to 3,500 mg/l were tested to obtain optimal PVA biodegradation activity of isolated strains, and the above two strains were found to degrade PVA up to 90%, when the initial concentration of PVA was 750 mg/l and below. Next, dyeing wastewater was tested by a nixed culture of the two isolated strains, and 42% and 55% of the initial concentrations of PVA and COD, respectively, was removed after five days. MLSS was gradually increased from an initial 1,400 to 2,500 mg/l, and the pH was also increased from 5.1 to 7.8. Sterilized dyeing wastewater was tested to find the effect of strains only on the biodegradation of PVA, and PVA degradation ratio and COD removal ratio were 50% and 72.8%, respectively. Thus, the results indicated that these two strains have good ability to degrade PVA and remove COD in dyeing wastewater, Finally, it is expected that if these two strains were used in the dyeing wastewater treatment, good efficiency for PVA degradation and COD removal could be achieved.

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References

  1. Chiellim, E., A. Corti, S. D' Antone, and R. Solara. 2003. Biodegradation of poly(vinyl alcohol)based materials. Prog. Polym. Sci. 28: 963- 1014.
  2. Choi, H. P., H. J. Kang, H. C. Seo, and H. C. Sung. 2002. Isolation and identification of photosynthetic bacterium useful for wastewater treatment. J. Microbiol. Biotechnol. 12: 643- 648.
  3. Choi, K. K., J. C. Shin, H. H. Jeon, S. Y. Kim, and J. W. Lee. 2003. Isolation and characterization of Microbacterium barkeri LCa and Paenibacillus amylolyticus LCb for PVA (polyvinyl alcohol) degradation. Korean J. Biotechnol. Bioeng. 18: 479- 484.
  4. Clescerl, L. S., A. E. Greenberg, and A. D. Eaton. 1998. Standard Methods for the Examination of Water and Wastewater. 20th Ed., pp. 2- 57. APHA,Washington U.S.A.
  5. Finley, J. H. 1961. Spectrophotometric determination of polyvinyl alcohol in paper coatings. Anal. Chem. 33: 1925-1927. https://doi.org/10.1021/ac50154a044
  6. Hashimoto, S. and M. Fujita. 1985. Isolation of a bacterium requiring three amino acids for polyvinyl alcohol degradation. J. Ferment. Technol. 63: 471-474.
  7. Jo, Y. L., S. Y. Jeong, and M. W. Cho. 1992. Isolation and characteristics of polyvinyl alcohol degrading bacteria. Kor. J. Appl. Microbiol. Biotechnol. 20: 96- 101.
  8. Ka, J. O. and I. H. Park. 2003. Isolation and characterization of 4-(2,4-dichlorophenoxy)butyric acid-degrading bacteria from agricultural soils. J. Microbiol. Biotechnol. 13: 243-250.
  9. Lee, K. Y. and C. G. Lee. 2002. Nitrogen removal from wastewaters by microalgae without consuming organic carbon sources. J. Microbiol. Biotechnol. 12: 979- 985.
  10. Lee, W. S., C. S. Park, J. E. Kim, B. D. Yoon, and H. M. Oh. 2002. Characterization of TCE-degrading bacteria and their application to wastewater treatment. J. Microbiol. Biotechnol. 12: 569- 575.
  11. Mori, T., M. Sakimoto, T. Kagi, and T. Sakai. 1996. Isolation and characterization of a strain of Bacillus megaterium that degrades poly(vinyl alcohol). Biosci. Biotech. Biochem. 60: 330- 332.
  12. Nord, F. F. 1936. Dehydrogenation activity of Fusarium lini B. Naturwissenschaften 24: 763.
  13. Park, C. H., T. H. Kim, S. Y. Kim, S. W. Kim, and J. W. Lee. 2002. Enhanced biodegradation of 2,4,6-trinitrotoluene (TNT) with various supplemental energy sources. J. Microbiol. Biotechnol. 12: 695- 698.
  14. Ryu, J. K., Y. J. Choi, and C. W. Lee. 1994. Studies on the optimal conditions for symbiotic utilization of polyvinyl alcohol by the KMG1 and KMG5 mixed culture and isolation of a thermophilic mutant of the PYA degrader KMG5. J. Kor. Soc. Water Quality 10: 112-119.
  15. Shimao, M., K. Ninomiya, N. Kato, and C. Sakazawa. 1986. Existence of a novel enzyme, pyrroloquinoline quinonedependent polyvinyl alcohol dehydrogenase, in a bacterial symbiont, Pseudomonas sp. strain VMI5C. Appl. Environ. Microbiol. 51: 268- 275
  16. Shimao, M., Y. Taniguchi, S. Shikata, N. Kato, and C. Sakazawa. 1982. Production of polyvinyl alcohol oxidase by a symbiotic mixed culture. Appl. Environ. Microbiol. 44: 28- 32.
  17. Sakazawa, C. and M. Shimao. 1981. Symbiotic utilization of polyvinyl alcohol by mixed cultures. Appl. Environ. Microbiol. 41: 261-267.
  18. Suzuki, T. 1976. Purification and some properties of polyvinyl alcohol degrading enzyme produced by Pseudomonas O-3. Agric. Biol. Chem. 40: 497- 504.
  19. Suzuki, T. 1977. Oxidation of secondary alcohols by polyvinyl alcohol-degrading enzyme produced by Pseudomonas O-3. Agric. Biol. Chem. 42: 1187-1194.